Ethanol, 2,2'-oxybis-, reaction products with ammonia, morpholine derivs. residues

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline with acceptable restrictions (partly limited documentation; post exposure observation period 7 days; purity unknown)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1969

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Principles of method if other than guideline:

Study is comparable with the inhalation hazard test described in the Annex of OECD Guideline 403 (adopted 1981)

GLP compliance:

no

Test type:

other: IHT

Test material

Test material information

Constituent 1

Reference substance name:

Ethanol, 2,2'-oxybis-, reaction products with ammonia, morpholine derivs. residues

EC Number:

272-712-1

EC Name:

Ethanol, 2,2'-oxybis-, reaction products with ammonia, morpholine derivs. residues

Cas Number:

68909-77-3

Molecular formula:

Not applicable (UVCB)

IUPAC Name:

2-(2-hydroxyethoxy)ethan-1-ol; 2-[1-(morpholin-4-yl)ethoxy]ethan-1-amine; 2-{2-[bis(2-hydroxyethyl)amino]ethoxy}ethan-1-ol; 4-{2-[2-(morpholin-4-yl)ethoxy]ethyl}morpholine; morpholin-3-one

Details on test material:

- Name of test material (as cited in study report): Morpholin-Rueckstand (morpholin remainder)
- Substance type: remainder mixture
- Physical state: liquid
- Analytical purity: no data

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gassner (Breeder)
- Mean body weight: 196 g

ENVIRONMENTAL CONDITIONS
not reported

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

Rats exposed for 8 h to a vapour saturated atmosphere.
Vapour was generated by bubbling 200 l/h dry air (no CO2) through the liquid substance column (volume ca. 50 ml) of about 5 cm above a fritted glass disc in a glass cylinder. The glass cylinder was heated in a water bath. Temperature in the exposure chamber was 20°C. There was no nominal substance loss detected by weighing.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

8 h

Concentrations:

There was no nominal substance loss detected by weighing. Therefore, no nominal concentration of the test atmosphere can be calculated.

No. of animals per sex per dose:

6

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: Weighing was done on d0 and d7. Observation was several times at the day of exposure and presumably daily exception of weekends and holidays afterwards.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

other: IHT (inhalation hazard test)

Exp. duration:

8 h

Remarks on result:

other: No mortality observed after exposure to a saturated atmosphere

Mortality:

0/12

Clinical signs:

other: slight irritation of the mucosa were observed only on the day of application

Body weight:

d0: 196 g; d7: 215 g

Gross pathology:

2* chronical bronchitis & bronchiektasia