Octane

Repeated dose toxicity: other routes

Administrative data

Endpoint:

short-term repeated dose toxicity: other route

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Adopted according to OECD SIDS (public available peer reviewed source) and respective study summary. The original source is not available and has not been reviewed.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Hepatotoxicity in albino rats exposed to n-octane and n-nonane.

Author:

Khan, S. and Pandya, K.

Year:

1985

Bibliographic source:

J Appl Toxicol 5: 64-68

Reference 2

Reference Type:

publication

Title:

Effect of n-octane and n-nonane administration on alkaline phosphatase activity in tissues of female rats.

Author:

Pandya, K. and Khan, S.

Year:

1982

Bibliographic source:

Biochem Pharmacol 31: 201-203

Reference 3

Reference Type:

secondary source

Title:

OECD SIDS - Category: C7-C9 Aliphatic hydrocarbon solvents

Author:

IHSC, American Chemistry Council

Year:

2009

Bibliographic source:

SIDS Initial Assessment Report For SIAM 30 Paris, 20-23 April 2010, drafted by members of the IHSC and reviewed by the U.S. Environmental Protection Agency (EPA), October 28, 2009

Report date:

2009

Materials and methods

Principles of method if other than guideline:

Purpose of the study was to evaluate enzyme activity and potential for hepatotoxicity. Animals were treated with octane for 2 or 7 days by intraperitoneal injections.

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Octane

EC Number:

203-892-1

EC Name:

Octane

Cas Number:

111-65-9

Molecular formula:

C8H18

IUPAC Name:

octane

Details on test material:

- Name of test material (as cited in study report): n-octane
- Analytical purity: 99%

Test animals

Species:

rat

Strain:

not specified

Sex:

female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

not specified

Analytical verification of doses or concentrations:

yes

Duration of treatment / exposure:

2 or 7 days

Frequency of treatment:

daily

Doses / concentrations

Remarks:

Doses / Concentrations:
1 mL/kg (approx. 703 mg/kg octane)

No. of animals per sex per dose:

10

Control animals:

yes

Results and discussion

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

Since only a single dose was administered it is not possible to derive a NOAEL.

 

Actual dose received by dose level by sex: 1.0 ml/kg Toxic response/effects by dose level: Statistically significant decreased activity in serum acetylcholinesterase and carboxylesterase were observed with an increase in aldolase after 2 and 7 days of treatment. After 7 days, a significant decrease in albumin (41%) total protein 34%) and total (42%) and esterified (60%) cholesterol was observed for n-octane. Free cholesterol was also increased after 7 days but nucleic acid content was not affected. Decreases in serum actylcholinesterase activity and albumin content may result from disturbances in secretory mechanism due to liver cell necrosis as suggested by the decrease in serum carboxylesterase. Changes in total and esterified cholesterol in serum and in hepatic free cholesterol suggest alteration in the liver esterification process. In an earlier publication by Pandya and Khan, 1980, administration of 1.0 mL/kg n-octane for 2 or 7 days resulted in increased alkaline phosphatase activity of liver, spleen and bone marrow. A single dose of hydrocarbon induced increases in spleen alkaline phosphatase lasting up to 42 days, while pretreatment with a protein synthesis inhibitor (cyclohexamide or ethionine) prevented the increase in alkaline phosphatase in liver and spleen.

 

Similar effects were observed for n-nonane.

Applicant's summary and conclusion

Conclusions:

n-Octane administered at only 1.0 mL/kg/day intraperitoneally for 2 or 7 days induced alterations in hepatic and serum enzymes and in concentrations of serum and liver lipids but did not alter nucleic acid content of liver cells.

Executive summary:

n-Octane administered at only 1.0 mL/kg/day intraperitoneally for 2 or 7 days induced alterations in hepatic and serum enzymes and in concentrations of serum and liver lipids but did not alter nucleic acid content of liver cells.