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EC number: 279-211-7 | CAS number: 79665-24-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Guideline study conducted in compliance with GLP regulations. According to the ECHA guidance document "Practical guide 6: How to report read-across and categories (March 2010)", the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
- Justification for type of information:
- See read across justification document in chapter 13
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The stock solution was shaken at room temperature with 20 rpm for 24 h (rotating shaker 3040, GFL) and membrane filtrated with 0.45 μm (RC, SCHLEICHER & SCHUELL).
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu)
- Age at study initiation: 2 - 24 h old
- Method of breeding: In 2-3 L glass vessels with approximatively 1.8 L culture medium, at 21 °C (temperatures of 20 - 25 °C are tolerated), in an
incubator, 16 h illumination, illumination strength max. 20 μmol photons⋅m-² ⋅s exp-1.
- Feeding: At least 5x weekly ad libitum with a mix of Desmodesmus subspicatus and Chlorella vulgaris,
with an algae cell density of > 10 exp6 cells/mL.
- Feeding during test: The daphnids were not fed during the study.
ACCLIMATION
- Acclimation period: ca. 2 h in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 18 - 22 °C, ± 1 °C constant
- pH:
- 7.86 test initial
7.81 - 7.85 end of test - Dissolved oxygen:
- 8.38 mg/L test initial
8.15 - 8.28 mg/L end of test - Nominal and measured concentrations:
- Test concentration (nominal): 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (5 cm ID x 8 cm H), 50 mL volume
- Material: The solution (100 mg/L test item was weighed out) was prepared with dilution water one day prior to application.
- Fill volume: 20 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: According to EC 92/69 L383A C.2. Annex
OTHER TEST CONDITIONS
- Photoperiod: 16/8 h light/dark cycle
- Light intensity:Diffuse light, illumination range max. 20 μmol photons⋅m-² ⋅ s exp-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobilisation of test organism - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: No acute toxicity within the range of solubility.
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Results with reference substance (positive control):
- 24 hours - EC50 (potassium dichromate) : 2.07 mg/L, (CL 1.68 - 2.56)
- Validity criteria fulfilled:
- yes
- Conclusions:
- No acute toxic effects occurred within the range of solubility. The test substance is with high probability acutely not harmful to aquatic invertebrates.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- 2004- 11-23 to 2004-11-25
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Guideline study conducted in compliance with GLP regulations. According to the ECHA guidance document "Practical guide 6: How to report read-across and categories (March 2010)", the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
- Justification for type of information:
- See read across justification document in chapter 13
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Water solubility < 1 mg/L
- pH value 7 (20 °C, 100 g/L) - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The stock solution was shaken at room temperature with 20 rpm for 24 h (rotating shaker 3040, GFL) and membrane filtrated with 0.45 μm (RC, SCHLEICHER & SCHUELL).
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu)
- Age at study initiation: 2 - 24 h old
- Method of breeding: In 2-3 L glass vessels with approximatively 1.8 L culture medium, at 21 °C (temperatures of 20 - 25 °C are tolerated), in an
incubator, 16 h illumination, illumination strength max. 20 μmol photons⋅m-² ⋅s exp-1.
- Feeding: At least 5x weekly ad libitum with a mix of Desmodesmus subspicatus and Chlorella vulgaris, with an algae cell density of > 10 exp6 cells/mL.
- Feeding during test: The daphnids were not fed during the study.
ACCLIMATION
- Acclimation period: ca. 2 h in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 18 - 22 °C, ± 1 °C constant
- pH:
- 7.85 test initial
7.83 - 7.85 end of test - Dissolved oxygen:
- 8.28 mg/L initial test
8.20 - 8.35 mg/L end of test - Nominal and measured concentrations:
- Test concentration (nominal): 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (5 cm ID x 8 cm H), 50 mL volume
- Material: The solution (100 mg/L test item was weighed out) was prepared with dilution water one day prior to application.
- Fill volume: 20 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: According to EC 92/69 L383A C.2. Annex
OTHER TEST CONDITIONS
- Photoperiod: 16/8 h light/dark cycle
- Light intensity:Diffuse light, illumination range max. 20 μmol photons⋅m-² ⋅ s exp-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobilisation of test organism - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Results with reference substance (positive control):
- 24 hours - EC50 (potassium dichromate) : 2.07 mg/L, (CI 1.68 - 2.56)
- Validity criteria fulfilled:
- yes
- Conclusions:
- No acute toxic effects occurred within the range of solubility. The test substance is with high probability acutely not harmful to aquatic invertebrates.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 2004- 11-23 to 2004-11-25
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Guideline study conducted in compliance with GLP regulations. According to the ECHA guidance document "Practical guide 6: How to report read-across and categories (March 2010)", the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Water solubility < 1 mg/L
- pH value 7 (20 °C, 100 g/L) - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The stock solution was shaken at room temperature with 20 rpm for 24 h (rotating shaker 3040, GFL) and membrane filtrated with 0.45 μm (RC, SCHLEICHER & SCHUELL).
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu)
- Age at study initiation: 2 - 24 h old
- Method of breeding: In 2-3 L glass vessels with approximatively 1.8 L culture medium, at 21 °C (temperatures of 20 - 25 °C are tolerated), in an
incubator, 16 h illumination, illumination strength max. 20 μmol photons⋅m-² ⋅s exp-1.
- Feeding: At least 5x weekly ad libitum with a mix of Desmodesmus subspicatus and Chlorella vulgaris, with an algae cell density of > 10 exp6 cells/mL.
- Feeding during test: The daphnids were not fed during the study.
ACCLIMATION
- Acclimation period: ca. 2 h in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 18 - 22 °C, ± 1 °C constant
- pH:
- 7.85 test initial
7.83 - 7.85 end of test - Dissolved oxygen:
- 8.28 mg/L initial test
8.20 - 8.35 mg/L end of test - Nominal and measured concentrations:
- Test concentration (nominal): 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (5 cm ID x 8 cm H), 50 mL volume
- Material: The solution (100 mg/L test item was weighed out) was prepared with dilution water one day prior to application.
- Fill volume: 20 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: According to EC 92/69 L383A C.2. Annex
OTHER TEST CONDITIONS
- Photoperiod: 16/8 h light/dark cycle
- Light intensity:Diffuse light, illumination range max. 20 μmol photons⋅m-² ⋅ s exp-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobilisation of test organism - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Results with reference substance (positive control):
- 24 hours - EC50 (potassium dichromate) : 2.07 mg/L, (CI 1.68 - 2.56)
- Validity criteria fulfilled:
- yes
- Conclusions:
- No acute toxic effects occurred within the range of solubility. The test substance is with high probability acutely not harmful to aquatic invertebrates.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Guideline study conducted in compliance with GLP regulations. According to the ECHA guidance document "Practical guide 6: How to report read-across and categories (March 2010)", the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The stock solution was shaken at room temperature with 20 rpm for 24 h (rotating shaker 3040, GFL) and membrane filtrated with 0.45 μm (RC, SCHLEICHER & SCHUELL).
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu)
- Age at study initiation: 2 - 24 h old
- Method of breeding: In 2-3 L glass vessels with approximatively 1.8 L culture medium, at 21 °C (temperatures of 20 - 25 °C are tolerated), in an
incubator, 16 h illumination, illumination strength max. 20 μmol photons⋅m-² ⋅s exp-1.
- Feeding: At least 5x weekly ad libitum with a mix of Desmodesmus subspicatus and Chlorella vulgaris,
with an algae cell density of > 10 exp6 cells/mL.
- Feeding during test: The daphnids were not fed during the study.
ACCLIMATION
- Acclimation period: ca. 2 h in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 18 - 22 °C, ± 1 °C constant
- pH:
- 7.86 test initial
7.81 - 7.85 end of test - Dissolved oxygen:
- 8.38 mg/L test initial
8.15 - 8.28 mg/L end of test - Nominal and measured concentrations:
- Test concentration (nominal): 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (5 cm ID x 8 cm H), 50 mL volume
- Material: The solution (100 mg/L test item was weighed out) was prepared with dilution water one day prior to application.
- Fill volume: 20 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: According to EC 92/69 L383A C.2. Annex
OTHER TEST CONDITIONS
- Photoperiod: 16/8 h light/dark cycle
- Light intensity:Diffuse light, illumination range max. 20 μmol photons⋅m-² ⋅ s exp-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobilisation of test organism - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: No acute toxicity within the range of solubility.
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Results with reference substance (positive control):
- 24 hours - EC50 (potassium dichromate) : 2.07 mg/L, (CL 1.68 - 2.56)
- Validity criteria fulfilled:
- yes
- Conclusions:
- No acute toxic effects occurred within the range of solubility. The test substance is with high probability acutely not harmful to aquatic invertebrates.
Referenceopen allclose all
Description of key information
Red disazo condensation pigments are with high probability acutely not harmful to aquatic invertebrates.
No toxic effects occur at the range of the substances solubility.
EC50 (48h) > 100 mg/L (nominal) for Daphnia magna (OECD 202)
Key value for chemical safety assessment
Additional information
The acute toxicity on aquatic invertebrates was investigated for two ‘red disazo condensation pigments’(CAS 40618-31-3 and CAS 52238-92-3) with the waterflea Daphnia magna, in GLP guideline studies according to OECD 202 [DR.U.NOACK-LABORATORIEN, 2004]. Based on the low water solubility of the test item, saturated stock solutions were prepared and the tests were conducted as limit tests with a nominal concentration of 100 mg/L. For both substances no effects on the swimming mobility of daphnia within the range of solubility are observed after 48 hours of exposure.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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