Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Appearence: colorless liquid
Purity: > 98%
Lot number: Lab 08 02

Test solutions

Details on test solutions:
Ultrapur water.
Water was ultrafiltered, passed on active carbon and ion exchange resin then filtered by a FG Millipore membrane (0.22 µm).

A saturated solution was prepared by puting 100 g of Bromo-1-pentane in 1 liter of solution under agitation during 18 hours with a temperature of 20°C.
The solutions with the concentration range were prepared by diluting the saturated solution with ultrapur water.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna Strauss cultived by the laboratory.
Organisms of less than 24 hours were selected by screening through a 0.56 mm bolt cloth.

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Post exposure observation period:
Not done

Test conditions

Hardness:
No data
Test temperature:
Between 19 and 21°C
pH:
pH variation between 7.50 and 7.91
Dissolved oxygen:
Concentrations between 8.0 and 8.7 mg/l
Salinity:
No data
Nominal and measured concentrations:
No data
Details on test conditions:
4 flasks of 5 daphnia were prepared for each concentration and the control test.
Reference substance (positive control):
yes
Remarks:
Dichromate de potassium: EC50- 24 h = 0.94 mg/l

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
4.5 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Not determined
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.9 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Not determined
Results with reference substance (positive control):
Immobilisation after 48 hours at 5%
Reported statistics and error estimates:
95% CL not detrmined because there are not enough points.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Bromo-1-pentane have a CE50 24h of 4.5 mg/l and a CE50 48h of 1.9 mg/l. It has been to classify as Dangerous for environment Tox 2.
Executive summary:

The study was conducted under EU Method C2

After 48 hours exposure to the test substance, the CE 50 was determined to be 1.9 mg/l.