Registration Dossier
Registration Dossier
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EC number: 266-357-1 | CAS number: 66422-95-5
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
LC50 -96H > 400 mg/L. LC0 -96H = 100 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 400 mg/L
Additional information
The acute toxicity of the test item on embryonic stages of zebrafish (Danio rerio) was determined according to the principles of the update of the OECD-guideline draft (2012). A semi-static test was conducted with the nominal concentrations of 25, 50, 100, 200 and 400 mg/L. Duration of the test was 96 hours with 24 hours renewal intervals. Twenty eggs were exposed to each concentration level of the test item and the reference item 3,4 -dichloroaniline. 48 eggs were exposed to the control with 24 internal controls which were not used for statistical analysis. The test was carried out in the dark. Water quality parameters pH-value, dissolved O2 -saturation and temperature were measured in freshly prepared test media (0, 24, 48 and 72H) and corresponding 24 hours aged test media (24, 48, 72 and 96H) from each test concentration, the control and the reference item. The water parameters were determined to be within acceptable limits. The valididity criteria of the guideline were fulfilled.
The concentrations of the test item solutions and control were analytically verified from two sampling intervals via LC-MS/MS. The measured concentrations in the freshly prepared media (0H and 72H) were in the range of 95 -107% and from 24H old solutions (24H and 96H) in the range of 99 -117% of the nominal concentrations, respectively. Due to the stability of the test item, the effect levels are given based on nominal concentrations of the test item. LC50 -96H > 400 mg/L. LC0 -96H = 100 mg/L.
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