Benzenethiol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzenethiol

EC Number:

203-635-3

EC Name:

Benzenethiol

Cas Number:

108-98-5

Molecular formula:

C6H6S

IUPAC Name:

benzenethiol

Details on test material:

- Name of test material : benzenthiol
- Molecular formula : C6H6S
- Molecular weight : 110.18
- Substance type: liquid (a clear, colorless)
- Analytical purity: =>98.0%
The determination of purity and molecular fomula were by IR , NMR and HPLC.
- Lot/batch No.:E99776L
- Storage condition of test material: at room temperature in the dark condition

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: -14.8℃
- Boiling point: 168℃
- Vapour pressure:0.13kPa (18℃)
- Water solubility (under test conditions): 836mg/L (25℃)
- log Pow:2.52
- pKa: 6.62

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling method: the solution was removed 1.5mL from test solution of vessel at the begining of test and after 24 hour exposure.

Test solutions

Vehicle:

yes

Details on test solutions:

- Preparetion of test solution:
(1)After adding test substances(2mg/L) to dilution water,each concentration of test solutions prepared .
(2)The positive control were dilution water only.
(3)Four test vessels were prepared each concentration of test solutions.Each vessels was added 100mL test solutions.
(4)All test vessels were checked it`s content. Test solutions of each concentraions were clear and colorless.

- Chemical name of vehicle (organic solvent, emulsifier or dispersant): M4 medium

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name:Daphnia magna
- Source: National Institute for Environmental Studies
- Age at study initiation : less than 24 hours old
- Method of breeding:20 Daphnia magnas/one concentration
- Feeding during test :no
- test water : volume: 100mL
- test water temparature: 20 ±1 ℃
- Light intensity : interior light ,16-hour light/8-hour dark cycle
- test Duration:48h

ACCLIMATION
- Acclimation period:14 days
- Acclimation conditions : same as test (test water temprature,Lighting)
- test water : M4 medium
- density of breeding : 20~50 animals/L test water
- Type and amount of food:0.1mg~0.2mgC/day/one Daphnia magna
- Health during acclimation :0% mortality observed

Study design

Test type:

semi-static

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

Not applicable

Test conditions

Hardness:

251mg/L(as CaCO3)

Test temperature:

20 ±1 ℃

pH:

pH7.6 ~ pH8.0 (0h)
pH7.4 ~ pH7.8 (24h)
M4 medium (test water) was pH8.0

Dissolved oxygen:

8.7~8.9 mg/L (0h)
8.4~8.8 mg/L (24 h)

Salinity:

Not applicable

Nominal and measured concentrations:

Not applicable

Details on test conditions:

TEST SYSTEM
- Test vessel:100mL glass vessel
- Medium: M4
- Renewal rate of test solution (frequency/flow rate): after 24h exposure
TEST CONCENTRATIONS
- Spacing factor for test concentrations:1.8
- Test concentrations: 0.0026mg/mL, 0.0047mg/mL, 0.0085mg/mL, 0.0150mg/mL, 0.0280mg/mL, 0.0500mg/mL
- Results used to determine the conditions for the definitive study: In result of range finding study showed 48hr - EiC50 was approximately 0.003mg/mL.
test substances concentrations ; 0.0026mg/mL, 0.0047mg/mL, 0.0085mg/mL, 0.0150mg/mL, 0.0280mg/mL, 0.0500mg/mL

TEST MEDIUM / WATER PARAMETERS
Stock culture medium: M4
following composition:
----------------------------
H3BO3: 2.860 mg/l
MnCl2.4H2O: 0.361 mg/l
LiCl: 0.306 mg/l
RbCl: 0.071 mg/l
SrCl2.6H20: 0.152 mg/l
NaBr: 0.016 mg/l
Na2MoO4.2H2O: 0.063 mg/l
CuCl2.2H2O: 0.017 mg/l
ZnCl2: 0.013 mg/l
CoCl2.6H20: 0.010 mg/l
KI: 0.00325 mg/l
Na2SeO3: 0.00219 mg/l
NH4VO3: 0.00058 mg/l
Na2EDTA.2H20: 2.5 mg/l
FeSO4.7H2O: 0.996 mg/l
MgSO4.7H2O: 123.3 mg/l
KCl: 5.8 mg/l
NaHCO3: 64.8 mg/l
NaNO3: 0.274 mg/l
KH2PO4: 0.143 mg/l
K2HPO4: 0.184 mg/l
Thiamine hydrochloride: 0.075 mg/l
Cyanocobalamine(V.B12): 0.001 mg/l
D(+)Biotin(V.H): 0.00075 mg/l
CaCl2.2H2O: 293.8 mg/l
Na2SiO3.9H2O: 10.0 mg/l
-------------------------------------

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.005 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

behaviour

Remarks on result:

other: 95% (0.0038~0.0068mg/L)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.004 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

behaviour

Remarks on result:

other: 95% (0.0014mg/L~0.0153mg/L)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.001 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other:

Details on results:

-test concentration in test solution : the begging of test; <0.0008~0.0186 mg/L (concentrations: 0.0026~0.0500 mg/L) , 24h after exposure ; <0.0008~0.0126 mg/L (concentrations 0.0026~0.0500mg/L);
-measured pH during test: pH7.4~pH8.0
-measured dissolved oxgen : 8.4mg/L~8.9mg/L ,all vessels confirmed more than 60% concentration of dissolved oxgen
-measured temperature of test solution : 19.5~20.5℃