Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2022
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 022
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Principles of method if other than guideline:
- This study was performed in order to evaluate the toxicity of the test item
tributyl(ethyl)phosphonium diphenyl phosphate in an acute immobilisation test under static
conditions towards Daphnia magna. Daphnia magna STRAUS, was chosen in the guideline
as a typical species of zoo-plankton.
Young daphnia, aged less than 24 hours at the start of the test, were exposed to the test
item for a period of 48 hours. Immobilisation was recorded at 24 hours and 48 hours and
compared with control values. Not more than 10 % of the control daphnia should show immobilisation or other signs of disease or stress, for example, discoloration or unusual behaviour such as trapping at surface of water. The results were analysed in order to calculate
the EC50 at 48 h.
A positive control was tested in a separate study to assure that the test conditions are reliable. - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Tributyl(ethyl)phosphonium diphenyl phosphate
- Cas Number:
- 2409816-82-4
- Molecular formula:
- C26H42O4P2
- IUPAC Name:
- Tributyl(ethyl)phosphonium diphenyl phosphate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
- Remarks:
- An analytical method for the determination of tributyl(ethyl)phosphonium diphenyl phosphate using IC was validated.
- Details on sampling:
- The 46 mg/L test item solutions were diluted as follows:
250 µL of 46 mg/L solution were mixed with 750 µL demineralised water resulting in a
4fold dilution. The lower tested concentrations were measured undiluted.
Test solutions
- Vehicle:
- not specified
- Details on test solutions:
- The concentration of the test item in the test solutions was determined as follows:
Linear calibration curve:
cmeasured [mg/L] = 【(Measured value - a)/b * 】*【100 %/
QC Recovery Rate [%] 】∗【 dilution factor】
with
a = intercept
b = slope
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- 18.5 hours before the start of the test, the adult animals were separated from the young. 0.5
hours before test start, the adults were caught with the help of a glass tube, and the newborn
daphnia (age < 24h) were sieved from the medium and immediately placed into a beaker
containing M4 medium. After the settling-in period, animals which showed no apparent damage were used for the test.
Study design
- Test type:
- not specified
- Water media type:
- other: Deionized water with an enrichment of certain minerals was used in the test.
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Duration: 48 hours
Observation times: 24 and 48 hours
Test conditions
- Test temperature:
- Temperature: 19.4 – 20.3 °C
within one test, temperature did not vary more than 2 °C - Details on test conditions:
Treatments: 1 / 2.2 / 4.6 / 10 / 22 / 46 mg/L (nominal concentration)
The concentrations to be tested were based on the result of
a non-GLP pre-test.
Temperature: 19.4 – 20.3 °C
within one test, temperature did not vary more than 2 °C
Photo period: 16/8 hours, using LED’s
Duration: 48 hours
Observation times: 24 and 48 hours
Medium renewal: none
Replicates (Treatments): 4 vessels, each containing 20 ± 5 mL test solution and 5
daphnia
Replicates (Blank control): 4 vessels, each containing 20 ± 5 mL M4 medium and 5
daphnia
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 13.13 mg/L
- 95% CI:
- >= 10.29 - <= 16.8
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- not specified
Any other information on results incl. tables
The estimation of the biological result was accomplished using the software ToxRat® Professional, version 3.3.0. The details of calculation are stated in the Annex 5: Statistical calculation using ToxRat® Professional 3.3.0.
The four highest tested concentrations showed toxicity between 5 and 100 % immobilization
after 48 hours. None of the animals were immobilised in the blank control.
The concentrations determined at the start of the test were in a range between 97 and 101 %
of the nominal concentration. At the end of the test the determined concentrations were
between 102 and 107 % of the nominal concentration. Therefore, the determination of the
biological results was based on the nominal concentration (see Guidance Doc. No.23
(§176)).
The biological results are presented in the following table:
Table 9 Biological Result of the Test Item
Parameter Value 95%-confidence interval
48h EC50 13.13 10.29 – 16.80
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- All validity criteria were fulfilled: Immobilisation in the controls may not exceed 10 %. Immobilisation in the control was 0 %. The concentration of dissolved oxygen at the end of the test must be at least 3 mg/L. The lowest concentration of dissolv
- Conclusions:
- The following result was determined for the test item tributyl(ethyl)phosphonium diphenyl
phosphate (species: Daphnia magna).
48h-EC50 = 13.13 mg/L (nominal) - Executive summary:
The study was performed using 6 concentrations ranging from 1 to 46 mg/L nominal concentration. For each test concentration and the blank control, 20 daphnia were exposed to
the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised
daphnia were counted.
The four highest tested concentrations showed toxicity between 5 and 100 % immobilization
after 48 hours. None of the animals were immobilised in the blank control.
3,5-Dichlorphenol (C6H4Cl2O, CAS No. 591-35-5) was used as positive control in a current
reference study to assure that the test conditions are reliable.
At the beginning and at the end of the test, the content of the test item in the test solutions
was determined using IC determination. The concentrations determined at the start of the
test were in a range between 97 and 101 % of the nominal concentration. At the end of the
test the determined concentrations were between 102 and 107 % of the nominal concentration. Therefore, the determination of the biological results was based on the nominal concentration (see Guidance Doc. No.23 (§176)).
The following result was determined for the test item tributyl(ethyl)phosphonium diphenyl
phosphate (species: Daphnia magna).
48h-EC50 = 13.13 mg/L (nominal)
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.