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Diss Factsheets
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EC number: 947-995-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06/2013 to 12/2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Guideline:
- other: The Guidelines for the Testing of Chemicals, State Environmental Protection Administration (SEPA) of China, 2004, No.201 "Algal Growth Inhibition Test".
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Rewosoft TE 19L; Batch ST02697671
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:
FORM AS APPLIED IN THE TEST (if different from that of starting material)
OTHER SPECIFICS: - Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- stock solution with test culture medium, prepared by 24 h shaking at 23°C
- Test organisms (species):
- Chlorella vulgaris
- Details on test organisms:
- TEST ORGANISM
- Common name: Chlorella vulgaris
- Strain:
- Source (laboratory, culture collection): Shanghai Fisheries University
- Age of inoculum (at test initiation):
- Method of cultivation:
ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not): sam
- Any deformed or abnormal cells observed: - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23 +- 2°C
- pH:
- 8.0 +- 0.2
- Nominal and measured concentrations:
- Preliminary test: 0.1, 1, 10, 50, 100 mg/L nominal
Definitive test: 5.0, 8.5, 14.5, 24.56, 41.8 mg/L nominal, 5.43, 8.48, 13.41, 25.10, 45.11 mg/L measured - Details on test conditions:
- TEST SYSTEM
- Test vessel: triangular flask
- fill volume: 300 mL
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density:
- Control end cells density: 2x10E4 cells/mL
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates):
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: distilled water
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium: no
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod:light cycle: 14h/10h
- Light intensity and quality: 4400 - 8800 Lux
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.7
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 0.1, 1, 10, 50, 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 20.34 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 9.02 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Reported statistics and error estimates:
- EC50 = 20.34 mg/L iwth 95 % confidence limit of 15.45 - 28.21 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- 72h-ErC50 = 20.34 mg/L
Reference
Concentration mg/L |
0 h [Algae cells/ml] |
24 h [Algae cells/ml] |
48 h [Algae cells/ml] |
72 h [Algae cells/ml] |
72h-I (%) |
0 | 10000 | 50000 | 180000 | 800000 | - |
10000 | 40000 | 170000 | 760000 | ||
10000 | 40000 | 160000 | 810000 | ||
5 | 10000 | 40000 | 170000 | 750000 | |
10000 | 40000 | 140000 | 740000 | 1.08 | |
10000 | 40000 | 160000 | 770000 | ||
8.5 | 10000 | 30000 | 150000 | 660000 | |
10000 | 40000 | 170000 | 630000 | 4.35 | |
10000 | 30000 | 130000 | 670000 | ||
14.5 | 10000 | 20000 | 50000 | 140000 | |
10000 | 40000 | 80000 | 180000 | 36.66 | |
10000 | 30000 | 70000 | 160000 | ||
24.5 | 10000 | 20000 | 30000 | 60000 | |
10000 | 20000 | 40000 | 70000 | 57.81 | |
10000 | 20000 | 30000 | 60000 | ||
41.8 | 10000 | 10000 | 10000 | 20000 | |
10000 | 10000 | 10000 | 10000 | 89.42 | |
10000 | 10000 | 10000 | 20000 |
Description of key information
72h-ErC50 = 20.34 mg/L
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 20.34 mg/L
- EC10 or NOEC for freshwater algae:
- 9.02 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.