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Diss Factsheets
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EC number: 435-680-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with GLP.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The actual concentration of the test substance was determined at the start and at the end of a 72 hours incubation period in filtered samples of the cultures exposed to the test substance and also in one blank containing 100.0 mg test substance per L test medium (nominal concentration), but no algae.
- Vehicle:
- no
- Details on test solutions:
- The test substance was dissolved in nutrient medium, consisting of sterile double distilled water and sterile concentrated nutrient medium.
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Selenastrum capricornutum ATCC (American Type Culture Collection) 22662, obtained directly from ATCC, Rockville, Maryland 20852, USA.
Deep frozen algae were thawed and cultivated as described by the supplier in 250 mL conical flasks (Erlenmeyer) each containing 100 ml medium for stock culture at a temperature of 23 ± 2 °C under permanent light with an intensity of at least 6000 lux. The culture was maintained under the same conditions. In about weekly intervals 1 mL of the stock culture was diluted 100-fold with nutrient medium for precultivation and incubation was continued. Test precultures were prepared by incubating a new stock culture for three days. At the start of the experiment the cell density was determined and adjusted to about 10^5 algae/mL by diluting with nutrient medium for precultivation. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None.
- Hardness:
- Water hardness corresponds to the standard test medium.
- Test temperature:
- 22 +- 1 °C.
- pH:
- The pH of the test culture at the start of the incubation period was lower than that of the controls (6.9 versus 8.3). 72 hours thereafter the pH of the test substance culture was 7.1 and was now comparable to the pH of 7.6 of the control culture. The pH of the blank containing test substance, but no algae, was 7.0 at the start and at the end of the incubation period.
- Dissolved oxygen:
- n.a.
- Salinity:
- N.a.
- Nominal and measured concentrations:
- Nominal: 100 mg/L.
Actual: At the start of the incubation, the actual test substance concentration in the test substance culture was 100.0 % of the nominal concentration. After 72 hours of incubation, the actual test substance concentration was 93.0 % of the actual starting concentration in the test substance culture. In the blank without algae, the actual test substance concentration was 99.7 % of the actual starting concentration. This indicates that the test substance was neither markedly degraded nor taken up by the algae during the incubation time of 72 hours.
Nutrient medium was used for the negative controls.
One blank containing 100.0 mg test substance per L test medium (nominal test substance concentration), but no algae, was incubated under the same culture conditions for analysis of the test substance concentration. - Details on test conditions:
- Sterile nutrient medium was used for the negative control cultures.
The test culture and the blank were set up in 250 ml conical flasks. The total culture volume was 100 ml in each case. The complete cultures each consisted of 10 ml inoculum (10^5 algae/ml) and 90 ml of the test substance stock solution or of the nutrient medium (control).
The blank contained 10 ml of nutrient medium (instead of the algal inoculum) and 90 ml of the test substance stock solution.
Incubated for three days in permanent light with an intensity of at least 6000 lux (wavelength 400-700 nm) while shaking. - Reference substance (positive control):
- not required
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Growth inhibition: The test substance did not markedly inhibit the growth of the algae during the incubation period of 72 hours (0.3 or 0.2 % inhibition, based on the area under the growth curves or based on the average growth rates).
- Results with reference substance (positive control):
- n.a.
- Reported statistics and error estimates:
- n.a.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the area under the growth curves or the average growth rate:
NOEC(0-72h) is >=100 mg/L.
EbC5072h >100 mg/L.
ErC5072h >100 mg/L. - Executive summary:
A Selenastrum capricornutum growth inhibition test according to the directive 92/69/EEC Part C.3 was performed to determine the possible effects of 7-AMCA on the growth of a unicellular green algal species.
A limit-test with one concentration of 100 mg of 7-AMCA per L test medium was performed. There were three replicates for each test and control culture. In each vessel the cell density of the algae was determined 24, 48 and 72 hours after the onset of incubation and the pH was measured at the onset and at the end of the incubation period. Possible test substance effects were determined by comparison of the areas under the growth curves and by comparison of the growth rates. The actual concentration of the test substance was determined at the start and at the end of a 72 hours incubation period in filtered samples of the cultures exposed to the test substance and also in one blank containing 100.0 mg test substance per L test medium (nominal concentration), but no algae.
Results
At the start of the incubation, the actual test substance concentration in the test substance culture was 100.0% of the nominal concentration. After 72 hours of incubation, the actual test substance concentration was 93.0 % of the actual starting concentration in the test substance culture. In the blank without algae, the actual test substance concentration was 99.7 % of the actual starting concentration. This indicates that the test substance was neither markedly degraded nor taken up by the algae during the incubation time of 72 hours. The pH of the test culture at the start of the incubation period was lower than that of the controls (6.9 versus 8.3). 72 hours thereafter the pH of the test substance culture was 7.1 and was now comparable to the pH of 7.6 of the control culture. The pH of the blank containing test substance, but no algae, was 7.0 at the start and at the end of the incubation period.
Increase in cell densities
During the 72 hours incubation period the cell density in the control culture was increased by a factor of about 60 (about 5.9 generations).
Growth inhibition
The test substance did not markedly inhibit the growth of the algae during the incubation period of 72 hours (0.3 or 0.2 % inhibition, based on the area under the growth curves or based on the average growth rates).
NOEC and EC50-values
Two "no observed effect concentrations" (NOECs) and two EC50 values were derived - based on the area under the growth curves and based on the average growth rates. As the actual test substance concentrations only minimally differed from the nominal ones and as they were stable throughout the incubation period the nominal starting concentrations are given: NOEC (0-72h) of 7-AMCA based on the area under the growth curves or the average growth rate is >=100 mg/L. EbC5072h >100 mg/L. ErC5072h >100 mg/L.
Reference
Validity of the test
The increase in cell density in the control cultures during the 72 hours incubation period was about 60-fold, i.e. more than 16-fold as recommended in the EC-guideline, corresponding to about 5.9 generations and showing optimal growth conditions for the algae.
The actual test substance concentrations at the end of the incubation period were higher than 80% of the actual starting concentration.
Description of key information
Based on the area under the growth curves or the average growth rate:
NOEC(0-72h) is >=100 mg/L.
EbC5072h >100 mg/L.
ErC5072h >100 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
The EC50 is not 100 mg/L, as one is forced to enter in the field above, but the EC50 is >100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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