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EC number: 215-960-8 | CAS number: 1461-25-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- not specified
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on test solutions:
- - Controls: 200 mL of pre-culture were filled into 250 mL Erlenmeyer flask. Then 8 mL of the nutrient solution and 42 mL aqua dem. were added.
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): Compounds in amounts of 2.5, 7.5, 15 mL and aqua dem was filled up to 250 mL - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- The activated sewage sludge for the inoculum was collected on the day before the start of the experiment from a well-operated municipal sewage treatment plant (Klaranlage Berlin-Ruhleben), predominantly dealing with domestic sewage (content of solid particles: 3.51 g/L). Upon arrival at the laboratory, the activated sludge was aerated and stirred for approximately 2 hours. Then it was left to settle for approximately 2 hours. 3000 mL were taken from the supernatant and mixed with 3000 mL water and 240 mL nutrient solution. The mixutre was aerated and stirred for approximately 19 hours.
The synthetic sewage feed was made by disolving 16 g peptone, 11 g meat extract, 3 g urea, 0.7 g sodium chloride, 0.4 g calcium chloride dihydrate, 0,2 g magnesium sulfate heptahydrate and 2.8 g dipotassium hydrogen phophate. - Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 30 min
- Remarks on exposure duration:
- Test vessels were incubated under stirring and aerating (45 L/h) for 30 minutes at room temperature.
- Test temperature:
- Room temperature, 22.7 to 24.1 degrees C after incubation
- Nominal and measured concentrations:
- 10, 20, 40, 100 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:aqua dem - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (5, 15 and 30 mg/L)
- Key result
- Remarks on result:
- other: the dissolved fraction of the test compound reduced the activiity to approx. 80 - 90 % of the control. Therefore, it can be concluded that Tetra-n-butyltin has some toxicity on the microbial population in sewage sludge.
- Results with reference substance (positive control):
- EC10 = 3.5 (2.4 - 4.7); EC50 = 12.3 (10.4-14.3), EC90 = 42.5 (33.-61.2)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The effects observed may be attributed to the dissolved fraction, since they were not concentration related. The differences in respiration rate inhibition between the concenrations of 10 to 100 mg/L are not relvant, since the variation between the duplicates is quite high. Only the highest concentration has a slightly higer inhibition rate. However, the results show that the dissolved fraction of the test compound reduced the activiity to approx. 80 - 90 % of the control. Therefore, it can be concluded that Tetra-n-butyltin has some toxicity on the microbial population in sewage sludge.
- Executive summary:
The purpose of this study was to determine the toxicity of the test compound ZK 24.942 (tetra-n-butyltin) on the microorganisms from a municipal sewage treatment plant over a period of 30 minutes. Study was conducted in agreement with OECD Guideline for Testing Chemicals No. 209 (activated sludge, respiration inhibition test). Results indicate a slight inhibitory effect on the activity of microorganisms from a municipal sewage treatment plant at the maximum solubility of the test compound (approx. 10 mg/L). However, the inhibition of respiration did not increase at higher nominal concentrations. It is therefore concluded that the effect can be attributed to the dissolved fraction of the compound.
Reference
Table 1: Respiration Inhibition
Test Compound |
Nominal Concentration (mg/L) |
Respiration Inhibition (%) |
3,5-Dichlorophenol |
5 |
17.7 |
15 |
58.4 |
|
30 |
82.1 |
|
Tetrabutyltin |
10 |
14.9 |
20 |
10.5 |
|
40 |
10.6 |
|
100 |
8.8 |
|
1000 |
19.3 |
In order to calculate the inhibitory effect of a test substance at a particular concentration, the respiration rate was expressed as a percentage of the mean of the three control respiration rates. The formula below was used to calculated the percentage of inhibition:
I = ((Rb - Rt)/Rb) x 100
where:
I = inhibition of respiration rate in per cent.
Rt = respiration rate at tested concentration of the test or reference substance
Rb = respiration rate of the control vessels (mean over three test vessels)
Description of key information
A dose related response was not seen with values of repeats differing significantly. Therefore no EC50 value could be determined for the key study. The lack of definitive result is likely to be due to the poor solubility of the test substance.
Key value for chemical safety assessment
Additional information
Toxicity to microorganisms.001: 30 minute exposure; results indicate a slight inhibitory effect on the activity of microorganisms from a municipal sewage treatment plant at the maximum solubility of the test compound
Toxicity to microorganisms.002: 18 hour EC50 = 1.7 mg/L
Toxicity to microorganisms.003: 30 minute EC50 = 1.1 µg/L
Toxicity to microorganisms.004: The EC50 (at 15 minutes) was 1.6 µmol/L in Vibrio fischeri
Toxicity to microorganisms.001 was conducted in accordance with GLP following OECD 209. It was therefore selected as the key study. A dose related response was not seen with values of repeats differing significantly. Therefore no EC50 value could be determined for this study.
Values from the supporting studies are wildly different from the key study and from each other. The variability of the results is likely to be due to the poor solubility of the test compound. The use of a vehicle is likely to aid solubility and this may help to explain why the supporting studies saw a dose related inhibitory effect unlike the key study.
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