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EC number: 816-315-9 | CAS number: 70024-75-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 17 - 19 Jan 2007
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- not specified
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fraction (WAF): This WAF was prepared by moderately stirring 100 mg test substance per liter of medium overnight followed by filtration (MILLIPORE AP15 glass fiber filter). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Length at study initiation (length definition, mean, range and SD): < 2mm
- Method of breeding: Parental and young Daphnia were held in 40L glass aquaria.
BREEDING
- Breeding conditions (same as test or not): Reconstituted water (ISO 6341) was the same as test, prepared with distilled water. The medium in the breeding system was continuously aerated in comparison to the test.
- Type and amount of food: 15 g sera micropan (sera GmbH, Heinsberg) homogenized in 1L deionized water
- Feeding frequency: Once a day, except weekends
ACCLIMATION
- Acclimation period: Daphnia, which had been selected in size, were acclimatized to the reconstituted water for 4 hours before introduction into the test media. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- Not specified
- Test temperature:
- 20.0 ± 0.5 °C
- pH:
- control: 7.3 at 0h, 7.7 at 48h
100 mg/l: 7.5 at 0h, 7.7 at 48h - Dissolved oxygen:
- control: 6.2 mg/l at 0h, 6.0 mg/l at 48h
100 mg/l: 6.0 mg/l at 0h, 5.9 mg/l at 48h - Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- nominal: 100 mg/L (WAF)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 100 mL flasks, all-glass, with 50 mL of test medium
- Aeration: no
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (ISO 6341), prepared with distilled water. The ratio of Ca:Mg ions is 4:1 and of Na:K ions is 10:1.
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Culture medium different from test medium: No, but test medium was not aerated as the culture medium.
- Intervals of water quality measurement: Temperature determined in a control flask at the start and at the end of the test. Oxygen and pH were determined in all vessels prior to the addition of Daphnia to the test medium and at the end of the test.
OTHER TEST CONDITIONS
- Photoperiod: 16 h a day, supplied by overhead white fluorescent tubes.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Observations of immobile Daphnia were made after 24 and 48 h of exposure - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 13 - 15 Dec 2006
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions (purity not reported, no analytical monitoring)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- not specified
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fraction (WAF): This WAF was prepared by moderately stirring 100 mg test substance per liter of medium overnight followed by filtration (MILLIPORE AP15 glass fiber filter).
- Controls: yes, water controls - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Length at study initiation: < 2mm
- Method of breeding: Parental and young Daphnia were held in 40L glass aquaria with a frequency of feeding once a day, except weekends, of 15 g sera micropan (homogenised in 1 L deionised water). The temperature was 20 ± 2 °C with an illumination of 16 h per day.
- Feeding during test: none
ACCLIMATION
- Acclimation period: 4 hours
- Acclimation conditions (same as test or not): same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- Not specified
- Test temperature:
- 20.0 ± 0.5 °C
- pH:
- control: 7.5 - 7.6
100 mg/L: 6.7 - 7.5 - Dissolved oxygen:
- control: 6.0 mg/L
100 mg/L: 5.8 - 6.0 mg/L - Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal: 0 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: all-glass, 100 mL flasks, with 50 mL of test medium
- Aeration: no
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water according to ISO 6341, prepared with distilled water.
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Na/K ratio: 10:1
- Culture medium different from test medium: No, but test medium was not aerated as the culture medium.
- Intervals of water quality measurement: Temperature determined in a control flask at the start and at the end of the test. Oxygen and pH were determined in all vessels prior to the addition of Daphnia to the test medium and at the end of the test.
OTHER TEST CONDITIONS
- Photoperiod: 16 h a day, supplied by overhead white fluorescent tubes.
EFFECT PARAMETERS MEASURED: Observations of immobile Daphnia were made after 24 and 48 h of exposure. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- mobility
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.
- Justification for type of information:
- Please see attached justification for read across in Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- mobility
Referenceopen allclose all
The test substance show no acute toxicity for daphnids under test conditions.
Table 1: Immobilisation of Daphnia magna after 24 and 48 hours
Nominal concentration (mg/l) |
Code |
Number of individuals per test vessel |
Immobile Daphnia (No./vessel) 24h |
Immobile Daphnia (No./vessel) 48h |
% immobile Daphnia after 24h of exposure |
% immobile Daphnia after 48h of exposure |
Control |
A B C |
20 |
0 |
0 |
0 |
0 |
100 |
A B |
20 |
0 |
1 |
0 |
5 |
Table 1: Acute immobilisation of Daphnia magna after 24 and 48 hours of exposure
Nominal concentration (mg/l) |
Code |
Number of individuals per test vessel |
Immobile Daphnia (No./vessel) 24h |
Immobile Daphnia (No./vessel) 48h |
% immobile Daphnia after 24h of exposure |
% immobile Daphnia after 48h of exposure |
Control |
A B C |
20 |
0 0 0 |
0 0 0 |
0 0 0 |
0 0 0 |
100 |
A B |
20 |
0 0 |
0 0 |
0 0 |
0 0 |
Description of key information
No effects up to the limit of water solubility for Daphnia magna (OECD 202); based on read-across from supporting substance (structural analogue or surrogate).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.