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EC number: 816-315-9 | CAS number: 70024-75-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions (purity not reported, no GLP data)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- not specified
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Station Epuration Wavre 2nd Stage, 23 Feb 1999
- Concentration of inoculum: > 1000000 CFU/mL (verification with Sanicheck) (CFU = Colony Forming Unit) - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Clearly chemically defined medium
- Test temperature: 22.1 - 22.4 °C
- pH: 7.2 - 7.3
- Aeration of dilution water: 24 h with CO2-free air (50 - 100 mL/min)
TEST SYSTEM
- Culturing apparatus: Container flask with 3 L of solution test and 30 mL inoculum
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: CO2-free air
- Details of trap for CO2 and volatile organics if used: The produced CO2 reacts with Ba(OH)2 to give a precipitate of Barium carbonate.
SAMPLING
- Sampling frequency: Sampling was performed on day 0, 2, 6, 9, 15, 19, 23 and 28.
- Sampling method: The produced CO2 was determinated in titrating the remaining Ba(OH)2 with HCl and phenolphtaleine.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 3 g/L
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 93.1
- Sampling time:
- 28 d
- Details on results:
- Within the 10-day time window the 60% level was passed. Therefore, the test substance is regarded as readily biodegradable.
- Results with reference substance:
- Degradation: 77.7% after 9 days, 60% pass level was reached.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 12 Sep - 10 Oct 2006
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions (purity not reported)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- not specified
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge from the aeration tank of a municipal waste water treatment plant, ARA Werdhölzli,
Zürich, Switzerland (06 Sep 2006)
- Pretreatment: The sludge was washed twice with tap water. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 22.6 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Aerobic mineral salts medium prepared with deionised water
- Test temperature: 22 ± 0.5 °C
- pH adjusted: Yes, the pH-value was checked at the beginning and at the end of the test and adjusted to pH 7.4 (± 0.2) with NaOH or HCL.
- Suspended solids concentration: 30 mg/L
- Continuous darkness: Yes, temperature-controlled dark room
TEST SYSTEM
- Culturing apparatus: 2500 mL closed glass bottle containing a total volume of test solution of 2000 mL
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Aerated with synthetic CO2-free air for a maximum test period of 28 days.
- Measuring equipment: Shimadzu 5050 TOC-Analyzer using the NPOC-mode for DOC-determination
- Details of trap for CO2: The air leaving the individual vessels was passed through gas-absorption bottles filled with NaOH. The trapped CO2 was determined as inorganic carbon (IC).
SAMPLING
- Sampling frequency: on Day 0, 1, 3, 7, 10, 14, 17, 21, 24 and 28
- Sampling method: Samples were centrifuged (15 min at 4500 g) and acidified to pH < 2. Prior to analysis the samples were sparged with CO2-free
high purity air for 10 min to remove inorganic carbon.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 2 replicates - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 20.3 mg/L (TOC)
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 90.2
- Sampling time:
- 28 d
- Details on results:
- Within the 10-day time window the 60% level was passed. Therefore, the test substance is regarded as readily biodegradable.
- Results with reference substance:
- Degradation: 83.1% after 14 days, 60% pass level was reached.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.
- Justification for type of information:
- Please see attached justification for read across in Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- GLP compliance:
- not specified
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 90.2 - <= 93.1
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
Referenceopen allclose all
Table 1: Biodegradation of test (mean of 2 replicates) and reference substance
Day |
Reference |
Test substance |
||
% CO2 produced |
%CO2 total |
% CO2 produced |
%CO2 total |
|
0 |
0.0 |
0.0 |
0.0 |
0.0 |
2 |
40.5 |
40.5 |
14.0 |
21.0 |
6 |
28.7 |
69.1 |
15.7 |
44.5 |
9 |
8.6 |
77.7 |
15.4 |
67.6 |
15 |
3.9 |
81.7 |
12.4 |
86.2 |
19 |
5.1 |
86.8 |
2.8 |
90.3 |
23 |
3.1 |
89.9 |
1.2 |
92.1 |
28 |
1.2 |
91.1 |
0.7 |
93.1 |
Table 2: Biodegradation of the replicates of the test substance
Day |
Test substance 1 |
Test substance 2 |
||
% CO2 produced |
%CO2 total |
% CO2 produced |
%CO2 total |
|
0 |
0.0 |
0.0 |
0.0 |
0.0 |
2 |
20.7 |
20.7 |
21.3 |
21.3 |
6 |
19.8 |
40.5 |
27.2 |
48.5 |
9 |
26.8 |
67.3 |
19.3 |
67.8 |
15 |
17.2 |
84.5 |
20.0 |
87.8 |
19 |
4.5 |
89.0 |
3.9 |
91.7 |
23 |
1.7 |
90.7 |
1.9 |
93.5 |
28 |
1.0 |
91.7 |
1.0 |
94.5 |
The biodegradation of the test substance reached 74% at the end of the 10-d window.
Table 1: Biodegradation of test substance
Time (days) |
Test substance 1 |
Test substance 2 |
|
||
Total CO2 release in test sample (mg IC/L) |
Degradation (%) |
Total CO2 release in test sample (mg IC/L) |
Degradation (%) |
Mean degradation (%) |
|
0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
1 |
0.0 |
0.0 |
-0.1 |
-0.1 |
0.0 |
3 |
1.7 |
4.3 |
2.0 |
5.7 |
5.0 |
7 |
10.3 |
37.0 |
8.7 |
30.2 |
33.6 |
10 |
16.0 |
58.4 |
15.3 |
55.4 |
56.9 |
14 |
19.9 |
75.5 |
20.0 |
75.9 |
75.7 |
17 |
21.1 |
81.4 |
21.3 |
82.4 |
81.9 |
21 |
23.0 |
86.4 |
22.8 |
85.6 |
86.0 |
24 |
23.1 |
86.8 |
24.0 |
90.6 |
88.7 |
28 |
23.7 |
88.1 |
24.6 |
92.2 |
90.2 |
Table 2: Biodegradation of reference substance and Inoculum blank
Time (days) |
Reference substance |
Inoculum blank |
|
Total CO2 release in test sample (mg IC/L) |
Degradation (%) |
Total CO2 release in test sample (mg IC/L) |
|
0 |
0.0 |
0.0 |
0.0 |
1 |
0.6 |
3.3 |
0.0 |
3 |
7.7 |
34.3 |
0.8 |
7 |
14.2 |
60.4 |
1.9 |
10 |
17.6 |
73.0 |
2.8 |
14 |
19.7 |
83.1 |
2.8 |
17 |
20.3 |
87.1 |
2.7 |
21 |
21.6 |
89.5 |
3.5 |
24 |
21.7 |
89.8 |
3.5 |
28 |
22.3 |
91.3 |
3.8 |
Description of key information
Readily biodegradable: 93.1% (CO2 evolution) in 28 days (OECD 301 B); based on read-across from supporting substance (structural analogue or surrogate).
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.