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Diss Factsheets
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EC number: 944-699-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: Ciba Geigy Method, please see more details below
- Deviations:
- not specified
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Details on test solutions:
- Not applicable
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Bacteria used have to be isolated from sewage sludge. This is taken from the aerated compartment of an Husmann apparatus and has to be prepared freshly on a
weekly basis. - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- Not available
- Test temperature:
- 25°C
- pH:
- not available
- Dissolved oxygen:
- Not available
- Salinity:
- Not applicable
- Conductivity:
- Not available
- Nominal and measured concentrations:
- Tested are 1, 5, 10, 20, 50, 100, 200 and 300 ppm of a test substance. In special cases 1, 3.2, 10, 32, 100, 320 and 1000 ppm are used (as indicated in the short report). 0.2 ml of the dilution is mixed with 15 ml of liquid nutrient agar (tempered to 45 °C) and plated on sterile petri dishes (agar incorporation test). Solvent and growth controls have to be used in parallel. In case of the suspension test 0.1 ml of dilutions are mixed with 4.5 ml of Nutrient broth in a test tube.
- Details on test conditions:
- Bacterial pre-cultures:
Once per week a pre-culture is prepared taking one inoculation loop of bacteria from an agar plate (BHI-agar) to be cultivated in Nutrient-Broth medium. Each day one inoculation loop from this pre-culture is used to inoculate 5 ml of Nutrient-Broth which is then cultured at 22 °C.
Method:
Inoculation suspension:
In case of tests on bacteria, the over-night culture is used as such.
In case of active sewage sludge testing, sludge is directly used from the Husmann apparatus on day 3 to 5. 100 ml
of underrun is taken on a daily basis and filtered through a Whatman GF/A filter. The filtrate is used as inoculation suspension.
Solvents:
Water, Methylcellusolve (< 2 %) or dimethyl formamide (< 1 %) are used as solvents.
Inoculation:
Agar incorporation test:
Prior to inoculation plates are dried 1-2 hours in an incubator. Plates are inoculated with 1 drop of bacteria suspension. As soon as the liquid has evaporated plates are closed and incubated for 12-24 hours at 25 ± 2 °C in an incubator.
Suspension test:
Test tubes are inoculated with one drop of the bacteria sewage sludge suspension and incubated for 12-24 hours
at 25 ± 2 °C in an incubator. - Reference substance (positive control):
- no
- Key result
- Duration:
- 21 d
- Dose descriptor:
- IC50
- Effect conc.:
- > 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: toxicity to activated sewage bacteria
- Details on results:
- Not available
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Not available
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The bacteria toxicity was determined to be greater than 400 mg/L.
- Executive summary:
The toxicity of test substance to activated sludge was determined in a 3-hours respiration inhibition test following procedure similar to the OECD Guideline No. 209.
The bacteria toxicity was determined to be greater than 400 mg/L.
Reference
Description of key information
The 3 hr IC50 for a isomeric mixture (FAT 40284) containing the target test substance FAT 36002 was found to be greater than 400 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 400 mg/L
Additional information
In two studies conducted 1n 1987 the toxicity of test substance to activated sludge was determined in a 3-hours respiration inhibition test following procedure similar to the OECD Guideline No. 209.
The bacteria toxicity data from both study was determined to be greater than 400 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.