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EC number: 700-956-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study performed under GLP; minor deviation not considered to impacted the reliability of the study.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- The temperature range during exposure was > 2°C; actual 2.5°C. This by expert judgement is not considered to have significantly affected the validity of the study.
- Qualifier:
- according to guideline
- Guideline:
- other: Chemical Registration Center of MEP. The Guidelines for the Testing of Chemicals, effects on Biotic Systems, 203 Fish Acute toxicity test [M]. (2013)
- Deviations:
- yes
- Remarks:
- see above
- Qualifier:
- according to guideline
- Guideline:
- other: China National Technical Committee for Standardization of Dangerous Chemicals Management: GB/T 27861-2011, Chemicals Fish Acute Toxicity Test. (2012)
- Deviations:
- yes
- Remarks:
- see above
- Qualifier:
- according to guideline
- Guideline:
- other: State Environmental Protection Administration of China: HJ/T 153-2004, The guidelines for the testing of chemicals. China (2004)
- Version / remarks:
- The test was conducted in accordance with China HJ/T153 - 2004 Chemical Test Guideline stipulations and Chemical Registration Center of MEP. The Guidelines for the Testing of Chemicals, effects on Biotic Systems, 203 Fish Acute toxicity test [M]. Second Edition. Beijing: China
Environmental Press. 2013: 30-36. The test was also completed under OECD TG 203: Fish Acute Toxicity Test (1992). - Deviations:
- yes
- Remarks:
- see above
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23 (2000)
- Deviations:
- not applicable
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 6.80 mg/L, 10.6 mg/L, 16.4 mg/L, 25.6 mg/L and 40.0 mg/L prepared as WAFs
- Sampling method: 50 ml extract from test solution.
- Sample storage conditions before analysis: Actual concentrations of test substance in hexyl hydride were immediately measured. Otherwise the samples (the hexyl hydride extractions) would be stored at 4°C until being analysed within one week. Upon which constituent 1 was measured as tracer. - Vehicle:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Strain: Not reported. Batch number FDr20141023-1
- Source: Recognised supplier (recorded in full study report) located in China.
- Age at study initiation (mean and range, SD): Not reported.
- Length at study initiation (length definition, mean, range and SD): Not reported. The body weight and total length of test fish in blank control were measured when the test was finished. The average and standard deviation of test fish body weight and total length were respectively 25.10 mm±0.87 mm and 0.126 g±0.008 g.
- Method of breeding: Not reported.
- Feeding during test
- Food type: No feeding during exposure. Not applicable.
- Amount: No feeding during exposure. Not applicable.
- Frequency: No feeding during exposure. Not applicable.
ACCLIMATION
- Acclimation period: 48 days
- Acclimation conditions (same as test or not): Yes. Same water quality, temperature 23 ± 2°C and photoperiod as used during the test (14 hours light; 10 hours dark).
- Type and amount of food: Not reported.
- Feeding frequency: At least three times a week until 24 hours before the start of the test.
- Health during acclimation (any mortality observed): None reported. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 118 mg/L expressed as CaCO3
- Test temperature:
- Temperature 21.3 - 23.8 °C during test period, the test was performed in a temperature controlled environment with continuous monitoring and was maintained at 23 ± 2°C.
- pH:
- Test water (dechlorinated water): pH 7.95.
The pH was in the range of 7.55 to 8.06 during the study. - Dissolved oxygen:
- The test solutions were not aerated during the exposure. The dissolved oxygen concentration in the test solutions during the test was kept at not Jess than 60 % of the air saturation value.
Dissolved oxygen (control): 8.7 mg/L. Dissolved oxygen (start; all test item concentrations): 8.3 - 8.7 mg/L. Dissolved oxygen (end 96-hours; all test item concentrations): 9.3 - 9.6 mg/L. - Nominal and measured concentrations:
- Preliminary range-finding test performed on static test system:
Nominal loading rates of 0 (blank control), 25.0 mg/L, 50.0 mg/L, and 100.0 mg/L prepared as WAFs
Definitive test performed on semi-static test system with renwal at 48 hours intervals:
Nominal loading rates of 0 (blank control), 6.80 mg/L, 10.6 mg/L, 16.4 mg/L, 25.6 mg/L and 40.0 mg/L prepared as WAFs
Otherwise indicated as: 2.08 mg/L, 3.24 mg/L, 5.03 mg/L, 7.85 mg/L and 12.2 mg/L calculated based on constituent 1 tracer; which in turn was measured (after and before the renewal) by Gas Chromatography-Mass Spectrometer (GC-MS) whereas the blank control was measured at 0h (new solution) and 48h (old solution). Measured concentrations are provided in 'Any other information on materials and methods incl. tables' field. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 5L vessel. Closed with plug.
- Material, size, headspace, fill volume: 4L test medium for each test concentration
- Aeration: None.
- Renewal rate of test solution (frequency/flow rate): Semi-Static; renewal 48 hour intervals.
- No. of organisms per vessel: 10 / test group (60 total).
- No. of vessels per concentration (replicates): no replicates; 1 exposure per test concentration
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Local tap water (dechlorinated tap water)
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH: None.
- Photoperiod: 14 hours light / 10 hours dark.
- Light intensity: Start: 4 lx, 76 lx and 92 lx (84 lx on average) / At the end of the test, the intensities of illumination of test environment were 92 lx, 84 lx and
88 lx (88 lx on average).
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortalities and abnormalities; measured at 3h, 24h, 48h, 72h and 96h as applicable.
TEST CONCENTRATIONS
- Test concentrations: test concentrations were based on the results of a range-finding tests. - Reference substance (positive control):
- yes
- Remarks:
- Potassium Dichromate
- Duration:
- 96 h
- Dose descriptor:
- LL0
- Effect conc.:
- 16.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- nominal loading rate WAF
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- 30.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- nominal loading rate WAF
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 28.1-33.3 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LL100
- Effect conc.:
- 40 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- nominal loading rate WAF
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: There were no abnormal responses of test fish in the blank control. Observed abnormal responses are reported in table 3.
- Observations on body length and weight: Body weight and length were recorded at the end of the observation period.
- Mortality of control: None.
- Other adverse effects control: Yes. None reported.
- Abnormal responses: Observed abnormal responses are reported in table 3.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None reported.
- Effect concentrations exceeding solubility of substance in test medium: Not applicable. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- 24h LC50: 252 mg/L (C.I. 223 -284 mg/L)
- Other: The results were kept at the value ranging from 200 mg/L to 400 mg/L, which met the criterion for the validity of the test. - Sublethal observations / clinical signs:
Table 1. Median Lethal Loading Rate (LL50) and 95% confidence limits
Exposure Duration
LL50 (mg/L) #1
C.I. 95% (mg/L) #1
24 h
32.0
Not available
48 h
32.0
Not available
72 h
30.6
28.1 – 33.3
96h
30.6
28.1 – 33.3
Note #1: means the median lethal loading rate was calculated based on the nominal loading rate
Table 2. Cumulative mortality
Nominal loading rate (mg/L)
Nominal loading rate constituent 1 (mg/L)
Number of organisms
Cumulative mortality (%)
3h
24h
48h
72h
96h
Blank control
0
10
0
0
0
0
0
6.89
2.08
10
0
0
0
0
0
10.60
3.24
10
0
0
0
0
0
16.40
5.03
10
0
0
0
0
0
25.60
7.85
10
0
0
0
10
10
40.0
12.2
10
60
100
100
100
100
Table 3. Observed abnormal responses
Nominal loading rate (mg/L)
Result of observations
Number affected / Total surviving ; Abnormal response (number)
3h
24h
48h
72h
96h
Blank Control
0/10
N
0/10
N
0/10
N
0/10
N
0/10
N
6.80
3/10
RA (3)
3/10
RA (3)
4/10
RA (4)
4/10
RA (4)
3/10
RA (3)
10.60
6/10
RA (6)
7/10
RA (5)
IMM(2)
10/10
RA (9)
AB (1)
10/10
RA (7)
IMM (3)
6/10
RA (6)
IMM (2)
16.40
8/10
AB (8)
10/10
RA (3)
(IMM7)10/10
RA (4)
AB (6)
10/10
RA (4)
LB (6)
8/10
RA (3)
IMM (7)
25.60
10/10
LB (3)
10/10
LB (8)
OLE(2)
10/10
LB (10)
9/9
LB (9)
9/9
LB (9)
40.00
4/4
LB (4)
4/4
-
-
-
-
-
-
-
Table 4. Abnormal responses descriptions of abbreviations
Abbreviation
Description
N
Normal
RA
Reduction in activity
IMM
Immobilised at the bottom
LB
Lying on the bottom
AB
Swimming at the bottom
OLE
Overturn and loss of equilibrium
Table 5. Measured concentration of constituent 1 (tracer) in test solutions
Nominal loading rate (mg/L)
Nominal loading rate constituent 1 (mg/L)
Measured concentration of the 1stconstituent of test substance (mg/L)
(Percent of measured concentrations versus the initial concentrations %)
Measured concentration in geometric mean
0h
48h
96h
New
Old
New
Old
-
Blank control
0
ND
ND
-
-
-
6.89
2.08
1.40
1.32 (94)
1.68
1.48 (88)
1.46
10.60
3.24
2.02
2.11 (104)
2.34
2.52 (108)
2.24
16.40
5.03
2.79
2.94 (105)
3.44
4.04 (117)
3.27
25.60
7.85
4.22
4.94 (117)
5.24
5.32 (102)
4.91
40.0
12.2
5.68
5.84 (103)
-
-
5.76
Where:
ND = lower than the LOD (LOD = 0.00411 mg/L)
-= test solution not measured.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 hour LL50 for the test substance to Danio rerio was determined to be 30.6 mg/L with a 95% confidence interval from 28.1 - 33.3 mg/L based on nominal loading rate as WAF.
- Executive summary:
The acute toxicity of the test item to Zebra fish (Danio rerio) was determined in a 96 hour semi-static test according to OECD TG 203 and the China CRC MEP guidelines, effects on Biotic Systems, 203 Fish Acute toxicity test (2013) under GLP. Based on the results of range finding tests, the nominal loading rates of 6.80, 10.6, 16.4, 25.6 and 40.0 mg/L WAFs with a geometric series factor of 1.56 were selected for exposure to 10 fish groups (60 total). One control containing no test substance was also performed. The test item was renewed at 48 hour intervals within a 96hour semi-static test system in response to ensuring adequate stability of exposure to test item. The actual concentrations were verified by measurement of constituent 1 used as a tracer in the nominal loading rates of 6.80, 10.6mg/, 16.4mg/, 25.6 and 40.0 mg/L WAFs at 0h, 96h, and 48h of new and old solutions (after and before the renewal) were measured by Gas Chromatography-Mass Spectrometer (GC-MS) the blank control was measured at 0h (new solution) and 48h (old solution) respectively. These measured concentrations ranged from 1.32 to 5.84mg/L (or 88% to 117% of the initial concentration constituent 1). During the renewal period of 48h, the measured concentrations of the tracer constituent in the old solutions could be maintained ±20% of the initial concentrations. Behavioural abnormalities and biological effects were reported during the course of the study. The 96h highest loading rate causing no mortality was 16.4 mg/L and the 96h median LL50 was 30.6 (C.I. 28.1 – 33.3) mg/L based on the nominal loading rate WAF.
Reference
Description of key information
LL50 (fish) = 30.6 (C.I. 28.1-33.3) mg/L based on WAF loading rates, 96hour, freshwater, OECD TG 203, 2015
LL0 (fish)= 16.4 mg/L based on loading WAF loading rates, 96hour, freshwater, OECD TG 203, 2015
Key value for chemical safety assessment
Additional information
The acute toxicity of the test item to Zebra fish (Danio rerio) was determined in a 96 hour semi-static test according to OECD TG 203 and the China CRC MEP guidelines, effects on Biotic Systems, 203 Fish Acute toxicity test (2013) under GLP. Based on the results of range finding tests, the nominal loading rates of 6.80, 10.6, 16.4, 25.6 and 40.0 mg/L WAFs with a geometric series factor of 1.56 were selected for exposure to 10 fish groups (60 total). One control containing no test substance was also performed. The test item was renewed at 48 hour intervals within a 96hour semi-static test system in response to ensuring adequate stability of exposure to test item. The actual concentrations were verified by measurement of constituent 1 used as a tracer. These measured concentrations ranged from 1.32 to 5.84mg/L (or 88% to 117% of the initial concentration constituent 1). During the renewal period of 48h, the measured concentrations of the tracer constituent in the old solutions could be maintained ±20% of the initial concentrations. Behavioural abnormalities and biological effects were reported during the course of the study. The 96h highest loading rate causing no mortality was 16.4 mg/L and the 96h median LL50 was 30.6 (C.I. 28.1 – 33.3) mg/L based on the nominal loading rate WAF.
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