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EC number: 477-690-9 | CAS number: 874819-71-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24/02/2022-14/10/2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Substance type: urease inhibitor
Molecular mass (g mol-1) 216.13
Analysed purity: 99.6%
Appearance: yellow solid (source: SDS) - Analytical monitoring:
- yes
- Details on sampling:
- The following specimen(s) were analysed in the analytical phase of the study:
Treatment Day 0, fresh Day 3, spent Day 5, fresh Day 7, spent
1 22ADR2-1 22ADR2-7 22ADR2-13 22ADR2-19
2 22ADR2-2 22ADR2-8 22ADR2-14 22ADR2-20
3 22ADR2-3 22ADR2-9 22ADR2-15 22ADR2-21
4 22ADR2-4 22ADR2-10 22ADR2-16 22ADR2-22
5 22ADR2-5 22ADR2-11 22ADR2-17 22ADR2-23
6 22ADR2-6 22ADR2-12 22ADR2-18 22ADR2-24
Treatment Day 12, fresh Day 14, spent Day 19, fresh Day 21, spent
1 22ADR2-25 22ADR2-31 22ADR2-37 22ADR2-43
2 22ADR2-26 22ADR2-32 22ADR2-38 22ADR2-44
3 22ADR2-27 22ADR2-33 22ADR2-39 22ADR2-45
4 22ADR2-28 22ADR2-34 22ADR2-40 22ADR2-46
5 22ADR2-29 22ADR2-35 22ADR2-41 22ADR2-47
6 22ADR2-30 22ADR2-36 22ADR2-42 22ADR2-48 - Vehicle:
- no
- Details on test solutions:
- The test item was soluble in the test medium. An aqueous stock solution was prepared by adding the test item to test medium, followed by treating with ultrasonic for 30 seconds and stirring for 10 minutes on a magnetic stirrer. The concentrations were prepared by serial dilutions of the stock solution A.
- the required volumes of the stock solution A were filled up to approximately 950 mL with test medium
- Appropriate amounts of algal suspension (dependent on the content of carbon C) were added and filled up to 1000 mL with test medium to ensure approximately 0.1 and 0.2 mg C/Daphnia/day was provided.
Test medium was prepared at least 1 day in advance and aerated for 2 hours before the 1st usage. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test organism: Daphnia magna STRAUS
Origin of the test organism: Landesanstalt für Umweltschutz Baden-Württemberg,
Griesbachstr. 1, 76185 Karlsruhe, Germany
Origin of the animals used in the test: holding and breeding occurred in the Test Facility under standardised laboratory conditions; test animals were derived from a healthy stock; no first brood progeny were used as test animals
Age of the Daphnia (at test start): < 24 hours and not first brood progeny
Breeding conditions: breeding was performed in glass beakers (50 mL) under the same conditions as in the test. Test animals were separated from the adults by means of a pipette.
Breeding medium: Elendt M4 medium
(according to the OECD Guideline 211)
Food: Alga Desmodesmus subspicatus purchased from MBM ScienceBridge GmbH, Hans-Adolf-Krebs-Weg 1, 37077 Göttingen, Germany,
Feeding: 3 times per week, depending on age the Daphnia were fed with a defined suspension of algae:
1 day old: 0.5 mL/50 mL M 4
3 to 10 days old: 1.0 mL/50 mL M 4
> 10 days old: 1.5 mL/50 mL M 4 - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- The water hardness was measured to be 335 mg/L CaCO3.
- Test temperature:
- 19.9 – 20.2 °C
- pH:
- 7.811-8.101
- Dissolved oxygen:
- 8.18-9.04 mg/L
- Conductivity:
- conductivity ≤ 10 µS/cm, measured: 0.05 µS/cm
- Nominal and measured concentrations:
- Test concentrations (nominal): 1.24, 3.71, 11.1, 33.3, 100.0 mg/L test item
Test concentrations
(time-weighted mean): 1.24, 3.73, 11.1, 33.3, 102.3 mg/L test item - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers (approximately 60 mL)
- Aeration: no aeration of the vessels
- Renewal rate of test solution (frequency/flow rate): The test medium was renewed 3 times per week.
- Number of parent (Daphnia)/test vessel (= replicate): 1
- Number of daphnids/concentration: 10
- Number of test vessels/concentration: 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:air-saturated deionized water (conductivity ≤ 10 µS/cm, measured: 0.05 µS/cm)
- Test medium: Elendt M4 medium
- Intervals of water quality measurement: Measurements of test medium parameters before test start and before each renewal.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light : 8 h dark, daily, light intensity: approximately 20 µE/m2s-1, equivalent to 1000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Time and parameters
At test start, test end and at least once a week after test start:
- pH, dissolved oxygen content*
- water hardness*
- test item concentrations
Daily from the appearance of first brood:
- Assessment of offspring and dead parent animals (counting)
- Recording the time to first brood in each test vessel will be assessed
The temperature was measured continuously. - Reference substance (positive control):
- no
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 102.3 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 102.3 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 102.3 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 6.5 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Development rate for reproduction
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.24 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Development rate for reproduction
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.73 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Development rate for reproduction
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.73 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality: Number of living offspring produced per surviving parental animal {for Daphnia magna, TG 211}
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.24 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality: Number of living offspring produced per surviving parental animal {for Daphnia magna, TG 211}
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 71.7 mg/L
- 95% CI:
- >= 41.8 - <= 161.7
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality: Number of living offspring produced per surviving parental animal {for Daphnia magna, TG 211}
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 1.97 mg/L
- 95% CI:
- >= 0.981 - <= 3.2
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality: Number of living offspring produced per surviving parental animal {for Daphnia magna, TG 211}
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.73 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality: Total number living offspring produced at the end of the test per parent daphnia at the start of the test excluding from the analysis parental accidental and/or inadvertent mortality {TG 211}
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.24 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality: Total number living offspring produced at the end of the test per parent daphnia at the start of the test excluding from the analysis parental accidental and/or inadvertent mortality {TG 211}
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 60.6 mg/L
- 95% CI:
- >= 33.1 - <= 160.8
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality: Total number living offspring produced at the end of the test per parent daphnia at the start of the test excluding from the analysis parental accidental and/or inadvertent mortality {TG 211}
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 2.04 mg/L
- 95% CI:
- >= 0.851 - <= 8.56
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality: Total number living offspring produced at the end of the test per parent daphnia at the start of the test excluding from the analysis parental accidental and/or inadvertent mortality {TG 211}
- Validity criteria fulfilled:
- yes
- Conclusions:
- A toxicity test was performed to assess the effects of the test item N-(2-Nitrophenyl) phosphoric triamide on the reproductive output of Daphnia magna during 21 days of exposure.
The measured test concentrations of the test item in test solutions ranged from 94 to 110% in fresh samples and from 65 to 117% in aged samples.
Based on the above, the results presented are based on the nominal concentrations for the test item as well as on the time-weighted mean concentrations.
The 21 day - NOEC, based on mean cumulative number of offspring per introduced parent, mean cumulative number of offspring per survived parent, development rate for reproduction, age of first reproduction and intrinsic rate of population increase is 1.24 mg/L test item (nominal concentration and TW mean).
The 21 day - NOEC, based on mortality of parents is ≥ 100.0 mg/L test item (equivalent to 102.3 mg/L test item, TW mean).
The EC10, EC20 and EC50 values were 2.04, 6.50 and 59.9 mg/L test item (equivalent to 2.04, 6.53 and
60.5 mg/L test item, TW mean) for mean cumulative number of offspring per introduced parent.
The EC10, EC20 and EC50 values were 1.97, 6.75 and 70.9 mg/L test item (equivalent to 1.97, 6.78 and
71.7 mg/L test item, TW mean) for mean cumulative number of offspring per survived parent.
The first offspring in control replicates was observed at day 10 after test start. No aborted brood or ephippia were observed in any treatment group. - Executive summary:
The primary objective of the test was to assess the effect of the test item on the reproductive output of Daphnia magna. The test duration was 21 days. At test end, the total number of living offspring produced was assessed. Data were analysed in order to determine the LOEC/NOEC values and to estimate concentrations that would cause a %-reduction in reproductive output (e.g. EC10, EC20 and EC50 values at 21 days), if possible. Furthermore, parental mortality was determined. Replicates were excluded if mortality was caused by technical reasons (mishandling or accident).
The test was performed using a semi-static test design. The study was conducted in accordance with the recommendations of the OECD Test Guideline No. 211 (2012).
The measured test concentrations of the test item in test solutions ranged from 94 to 110% in fresh samples and from 65 to 117% in aged .
Based on the above, the results presented are based on the nominal concentrations for the test item as well as on the time-weighted mean concentrations.
The first offspring in control replicates was observed at day 10 after test start.
Based on the above, the results presented are based on the nominal concentrations for the test item as well as on the time-weighted mean concentrations.
The 21 day - NOEC, based on mortality of parents is ≥ 100.0 mg/L test item (equivalent to 102.3 mg/L test item, TW mean).
The EC10, EC20 and EC50 values were 2.04, 6.50 and 59.9 mg/L test item (equivalent to 2.04, 6.53 and
60.5 mg/L test item, TW mean) for mean cumulative number of offspring per introduced parent.The EC10, EC20 and EC50 values were 1.97, 6.75 and 70.9 mg/L test item (equivalent to 1.97, 6.78 and 71.7 mg/L test item, TW mean) for mean cumulative number of offspring per survived parent.
Table 1: Effects on reproduction of Daphnia magna: cumulative number of offspring and development rate
Effect concentration
mg/L N-(2-Nitrophenyl) phosphoric triamide
Mean cumulative number of offspring per introduced parent 21 days after application
Mean cumulative number of offspring per survived parent 21 days after application
Development rate for reproduction
NOEC
test item, nominal
1.24
1.24
1.24
test item, TW mean
1.24
1.24
1.24
LOEC
test item, nominal
3.71
3.71
3.71
test item, TW mean
3.73
3.73
3.73
EC10
EC10
EC10
EC10
and 95 % confidence intervals
(lower – upper)
test item, nominal
2.04
1.97
6.48
(0.830 – 3.59)
(0.966 – 3.22)
(n.d.)
test item, TW mean
2.04
1.97
6.50
(0.851 – 3.56)
(0.981 – 3.20)
(n.d.)
EC20
and 95 % confidence intervals
(lower – upper)
test item, nominal
6.50
6.75
n.d.
(3.71 – 10.5)
(4.31 – 10.1)
test item, TW mean
6.53
6.78
n.d.
(3.77 – 10.5)
(4.36 – 10.0)
EC50
and 95 % confidence intervals
(lower – upper)
test item, nominal
59.9
70.9
n.d.
(32.5 – 163.6)
(41.1 – 162.8)
test item, TW mean
60.6
71.7
n.d.
(33.1 – 160.8)
(41.8 – 161.7)
Table 2: Effects on reproduction of Daphnia magna: mortality of parents, age of first reproduction
and intrinsic rate of population increase
Effect concentration
mg/L N-(2-Nitrophenyl) phosphoric triamide
Mortality of parents
Age of first reproduction
Intrinsic rate of population increase*
NOEC
test item, nominal
≥ 100.0
1.24
1.24
test item, TW mean
≥ 102.3
1.24
1.24
LOEC
test item, nominal
> 100.0
3.71
3.71
test item, TW mean
> 102.3
3.73
3.73
EC10
and 95 % confidence intervals
(lower – upper)
test item, nominal
> 100.0
n.d.
9.35
(3.98 – 22.0)
test item, TW mean
> 102.3
n.d.
9.28
(3.93 – 21.9)
EC20
and 95 % confidence intervals
(lower – upper)
test item, nominal
n.d.
n.d.
44.8
(17.3 – 120.7)
test item, TW mean
n.d.
n.d.
45.1
(17.4 – 122.6)
EC50
and 95 % confidence intervals
(lower – upper)
test item, nominal
n.d.
n.d.
n.d.
test item, TW mean
n.d.
n.d.
n.d.
Reference
Results for reproduction and mortality of Daphnia magna
Treatment group | mg/L N-(2-Nitrophenyl) phosphoric triamide | |||||
| control | 1.24 | 3.71 | 11.1 | 33.3 | 100.0 |
| mg/L test item, TW mean | |||||
| control | 1.24 | 3.73 | 11.1 | 33.3 | 102.3 |
21 days after application | Cumulative number of living offspring | |||||
|
|
|
|
|
|
|
Mean number per introduced parent | 90.6 | 91.7 | 72.1 | 66.4 | 50.7 | 32.7 |
SD | 8.36 | 7.06 | 10.95 | 9.85 | 6.93 | 13.16 |
CV (%) | 9.2 | 7.7 | 15.2 | 14.8 | 13.7 | 40.2 |
|
|
|
|
|
|
|
Mean number per survived parent | 90.6 | 91.7 | 72.1 | 66.4 | 50.7 | 36.3 |
SD | 8.36 | 7.06 | 10.95 | 9.85 | 6.93 | 6.80 |
CV (%) | 9.2 | 7.7 | 15.2 | 14.8 | 13.7 | 18.7 |
| Reduction (%) in cumulative number of offspring per introduced parent1 relative to the control | |||||
| n.r. | -1.2 | 20.4 + | 26.7 + | 44.0 + | 63.9 + |
| Reduction (%) in cumulative number of offspring per survived parent1 relative to the control | |||||
| n.r. | -1.2 | 20.4 + | 26.7 + | 44.0 + | 59.9 + |
| Mortality of parent daphnids (%) | |||||
| 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 10.0 |
| Age of first reproduction (Days)1 | |||||
| 10.8 | 11.1 | 11.8 | 11.1 | 12.8 | 13.1 |
| Development rate for reproduction | |||||
| 0.093 | 0.090 | 0.085 | 0.090 | 0.078 | 0.077 |
| Inhibition of development rate for reproduction (%)1 relative to the control | |||||
| n.r. | 2.7 | 8.0 + | 2.5 + | 15.4 + | 17.3 + |
| Mean intrinsic rate (r) of population increase | |||||
| 0.313 | 0.303 | 0.275 | 0.290 | 0.257 | 0.224 |
| Inhibition of intrinsic rate (r) of population increase (%)1 relative to the control | |||||
| n.r. | 3.1 | 12.1 + | 7.2 + | 18.0 + | 28.5 + |
Effects on reproduction of Daphnia magna: cumulative number of offspring and development rate
Effect concentration | mg/L N-(2-Nitrophenyl) phosphoric triamide | ||
Mean cumulative number of offspring per introduced parent 21 days after application | Mean cumulative number of offspring per survived parent 21 days after application | Development rate for reproduction | |
NOEC |
|
|
|
test item, nominal | 1.24 | 1.24 | 1.24 |
test item, TW mean | 1.24 | 1.24 | 1.24 |
|
|
|
|
LOEC |
|
|
|
test item, nominal | 3.71 | 3.71 | 3.71 |
test item, TW mean | 3.73 | 3.73 | 3.73 |
|
|
|
|
EC10 | EC10 | EC10 | EC10 |
and 95 % confidence intervals |
|
|
|
(lower – upper) |
|
|
|
|
|
|
|
test item, nominal | 2.04 | 1.97 | 6.48 |
| (0.830 – 3.59) | (0.966 – 3.22) | (n.d.) |
test item, TW | 2.04 | 1.97 | 6.50 |
| (0.851 – 3.56) | (0.981 – 3.20) | (n.d.) |
|
|
|
|
EC20 |
|
|
|
and 95 % confidence intervals |
|
|
|
(lower – upper) |
|
|
|
|
|
|
|
test item, nominal | 6.50 | 6.75 | n.d. |
| (3.71 – 10.5) | (4.31 – 10.1) |
|
test item, TW mean | 6.53 | 6.78 | n.d. |
| (3.77 – 10.5) | (4.36 – 10.0) |
|
|
|
|
|
EC50 |
|
|
|
and 95 % confidence intervals |
|
|
|
(lower – upper) |
|
|
|
|
|
|
|
test item, nominal | 59.9 | 70.9 | n.d. |
| (32.5 – 163.6) | (41.1 – 162.8) |
|
test item, TW mean | 60.6 | 71.7 | n.d. |
| (33.1 – 160.8) | (41.8 – 161.7) |
|
|
|
|
|
Effects on reproduction of Daphnia magna: mortality of parents, age of first reproduction and intrinsic rate of population increase
Effect concentration | mg/L N-(2-Nitrophenyl) phosphoric triamide | ||
Mortality of parents | Age of first reproduction | Intrinsic rate of population increase* | |
NOEC |
|
|
|
test item, nominal | ≥ 100.0 | 1.24 | 1.24 |
test item, TW mean | ≥ 102.3 | 1.24 | 1.24 |
|
|
|
|
LOEC |
|
|
|
test item, nominal | > 100.0 | 3.71 | 3.71 |
test item, TW mean | > 102.3 | 3.73 | 3.73 |
|
|
|
|
EC10 |
|
|
|
and 95 % confidence intervals |
|
|
|
(lower – upper) |
|
|
|
test item, nominal | > 100.0 | n.d. | 9.35 |
|
|
| (3.98 – 22.0) |
test item, TW mean | > 102.3 | n.d. | 9.28 |
|
|
| (3.93 – 21.9) |
|
|
|
|
EC20 |
|
|
|
and 95 % confidence intervals |
|
|
|
(lower – upper) |
|
|
|
test item, nominal | n.d. | n.d. | 44.8 |
|
|
| (17.3 – 120.7) |
test item, TW mean | n.d. | n.d. | 45.1 |
|
|
| (17.4 – 122.6) |
|
|
|
|
EC50 |
|
|
|
and 95 % confidence intervals |
|
|
|
(lower – upper) |
|
|
|
test item, nominal | n.d. | n.d. | n.d. |
test item, TW mean | n.d. | n.d. | n.d. |
|
|
|
|
Calculations were conducted using unrounded values
TW mean - time-weighted mean
* The intrinsic rate is considered as a measure of population growth based on reproductive output and age-specific mortality. In steady state populations the intrinsic rate of population increase is zero, for growing populations it is positive and for shrinking populations it is negative.
n.d. - not determined due to mathematical reasons or inappropriate data
Description of key information
A toxicity test was performed to assess the effects of the test item N-(2-Nitrophenyl) phosphoric triamide on the reproductive output of Daphnia magna during 21 days of exposure.
The measured test concentrations of the test item in test solutions ranged from 94 to 110% in fresh samples and from 65 to 117% in aged samples.
Based on the above, the results presented are based on the nominal concentrations for the test item as well as on the time-weighted mean concentrations.
The 21 day - NOEC, based on mean cumulative number of offspring per introduced parent, mean cumulative number of offspring per survived parent, development rate for reproduction, age of first reproduction and intrinsic rate of population increase is 1.24 mg/L test item (nominal concentration and TW mean).
The 21 day - NOEC, based on mortality of parents is ≥ 100.0 mg/L test item (equivalent to 102.3 mg/L test item, TW mean).
The EC10, EC20 and EC50 values were 2.04, 6.50 and 59.9 mg/L test item (equivalent to 2.04, 6.53 and
60.5 mg/L test item, TW mean) for mean cumulative number of offspring per introduced parent.
The EC10, EC20 and EC50 values were 1.97, 6.75 and 70.9 mg/L test item (equivalent to 1.97, 6.78 and
71.7 mg/L test item, TW mean) for mean cumulative number of offspring per survived parent.
The first offspring in control replicates was observed at day 10 after test start. No aborted brood or ephippia were observed in any treatment group.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- 1.24 mg/L
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