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EC number: 437-930-5 | CAS number: 51553-03-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03.02.2020 - 31.03.2020
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 437-930-5
- EC Name:
- -
- Cas Number:
- 51553-03-8
- Molecular formula:
- Hill formula: C22H23N7O5 CAS formula: C22H23N7O5
- IUPAC Name:
- N-{5-[bis(2-methoxyethyl)amino]-2-[2-(2,6-dicyano-4-nitrophenyl)diazen-1-yl]phenyl}acetamide
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The samples were filled into glass vials, stabilised with 50% acetonitrile and stored in the freezer (≤ -18 °C). The sampling and documentation were conducted according to the following specification: After 0 h, 24 h and 48 h exposure, two replicates of 4 ml each were sampled from NC and A. Per replicate 1 ml of each test vessel were pooled to obtain 4 ml in total. The samples were filled into 12 mL-glass screw-cap vials. To each vial, 4 mL acetonitrile was added and samples were stored in the freezer (≤ -18 °C). Of each sampled treatment, one of the samples from 0 h, 24 h and 48 h was sent to the analytical laboratory at the test site biochemA GmbH for chemical analysis using an insulated box with thermal packs. The remaining samples were stored as retain samples in the freezer (≤ -18 °C) until finalisation of the study.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Test item concentration was below the limit of calibration (LOC = 1 mg/L) in the saturated test item solution as well as in the negative control. Therefore, effect concentrations could not be calculated based on exposure concentrations but are given in relation to the nominal loading rate.
Test organisms
- Test organisms (species):
- other: Daphnia magna Strauss
- Details on test organisms:
- The test organism Daphnia magna Straus originates from the clone breeding of the Federal Environment Agency, department IV 2.4, Berlin, and has been cultivated at Hydrotox GmbH since October 2012. The daphnids are cultured at 20 2 °C with 16 h light : 8 h dark and placed into fresh Elendt M4 medium twice a week. They are kept at approx. 10 animals / 200 mL and fed on workdays with Desmodesmus subspicatus algae corresponding to 0.1 mg C × daphnid-1 × day-1. The test organisms used were 1.75 – 21.25 h old at the start of the test.
Study design
- Test type:
- static
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- The swimming capability of the daphnids was assessed after 24 h and 48 h exposure.
Test conditions
- Test temperature:
- 20.2 - 21.3 °C
- pH:
- 7.6 - 7.7
- Dissolved oxygen:
- 7.5 - 8.8 mg/L
- Conductivity:
- conductivity (695 μS/cm)
- Nominal and measured concentrations:
- Test item concentration was below the limit of calibration (LOC = 1 mg/L) in the saturated test item
solution as well as in the negative control. Therefore, effect concentrations could not be calculated
based on exposure concentrations but are given in relation to the nominal loading rate. - Details on test conditions:
- - The pH was 7.6 – 7.7 in the control as well as in the test item treatment (recommended: 6 – 9).
- The dissolved oxygen concentration was 7.5 – 8.8 mg/L in the control and 7.3 – 8.7 mg/L in the test item treatment (required: ≥ 3 mg/L).
- The temperature during the exposure was 20.2 – 21.3 °C (required: 18 – 22 °C, constant within +- 1 °C).
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LOELR
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOELR
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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