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EC number: 289-530-3 | CAS number: 89899-25-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Details on test solutions:
- 100.03 mg of the test item was weighed and made up to 100 ml using 20X-AAP growth medium to make a stock concentration of 1 mg/ml. All the required test concentrations were prepared for 800 ml. From the stock solution, each required test concentration was prepared as follows: 800 µl, 1760 µl, 3872 µl, 8520 µl, 18744 µl and 41232 µl were taken and made up to 800 ml with 20X-AAP medium to obtain 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L concentrations respectively. For Control, 20X-AAP medium was used.
- Test organisms (species):
- Lemna gibba
- Details on test organisms:
- TEST ORGANISM
- Strain: Lemna gibba (Duckweed)
- Source (laboratory, culture collection): In-house culture, RCC Laboratories India Private Limited (Originally procured by Department of Plant Biology & Plant Biotechnology Madras Christian College, Chennai) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 7 d
- Hardness:
- no data available
- Test temperature:
- Temperature in test vessel (°C) Start of the test
Control : 23.5
Treatment : 23.5
End of the test
Control : 24.8-25.1
Treatment : 25.1-25.3 - pH:
- Medium pH range Start of the test
Control : 7.54
Treatment : 7.48
End of the test
Control : 8.69-8.72
Treatment : 8.72-8.75 - Dissolved oxygen:
- no data available
- Salinity:
- no data available
- Conductivity:
- no data available
- Nominal and measured concentrations:
- 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 ml Glass Beaker
- No. of organisms per vessel: 12 fronds (3 plants)
- No. of vessels per concentration (replicates): Main Experiment - 3 replicates for each concentration
- No. of vessels per control (replicates): Control - 6 Replicates.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: Start of the test
- Photoperiod: Continuous Light
- Light intensity and quality: 8460-8650 Lux - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 21.12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 9.55 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 21.67 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Frond Dry Weight; Growth
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 8.69 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Frond Dry Weight; Yield
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Frond number and dry weight
- Details on results:
- None
- Results with reference substance (positive control):
- ErC50 of 3,5-Dichlorophenol based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was calculated as 4.30 mg/L with a lower confidence limit of 4.15 mg/L and the upper confidence limit of 4.46 mg/L.
EyC50 of 3,5-Dichlorophenol based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was calculated as 2.38 mg/L with a lower confidence limit of 2.29 mg/L and the upper confidence limit of 2.48 mg/L.
ErC50 of 3,5-Dichlorophenol based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was calculated as 3.02 mg/L with a lower confidence limit of 2.89 mg/L and the upper confidence limit of 3.16 mg/L.
EyC50 of 3,5-Dichlorophenol based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was calculated as 1.53 mg/L with a lower confidence limit of 1.46 mg/L and the upper confidence limit of 1.59 mg/L. - Reported statistics and error estimates:
- Mean coefficient of variation of average specific growth rate in replicate control and treated cultures were calculated. No analysis was done as the study is concluded with the Limit Test.
- Validity criteria fulfilled:
- yes
- Conclusions:
- ErC50 of FAT 20290/E TE based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was found to be 21.12 mg/L.
- Executive summary:
Lemna gibba (Duckweed) were exposed to the test item (FAT 20290/C TE) at different test concentrations viz 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L. Initially on day ‘0’, 12 fronds were inoculated in beaker containing 200 ml of test medium (20X-AAP medium). Three replicates for each test concentration and six replicates for control were maintained. The test item was formulated in 20X-AAP medium. Prior to exposure, Lemna gibba was pre-cultured in the 20X-AAP medium for period of ‘8’ days and the pre-culture was found healthy. After exposure, Lemna gibba was observed for frond count on days ‘3’, ‘5’ and ‘7’. Dry weight of the fronds were recorded at start (pre-culture representative samples) and at the end of the test. Fronds were observed for their appearance on days ‘3’, ‘5’ and ‘7’. All fronds were found normal and healthy in all replicates of control and test concentrations except 51.54 mg/L where necrosis was observed on day 5 and in addition to necrosis, root filaments were turned blue in 10.65, 23.43 and 51.54 mg/L concentrations on day 7. The doubling time (Td) of frond number in the control group was calculated as 1.62 day-1.
Frond Numbers:
From the average specific growth rate recorded with the different test concentrations viz 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, the test concentration that inhibited 50% growth (ErC50) was calculated as 21.12 mg/L with upper confidence limit of 22.14 mg/L and lower confidence limit of 20.14 mg/L. The percent inhibition on the average specific growth rate on day ‘7’ for the test concentrations of 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, was calculated as 0.12, 0.11, 8.33, 34.62, 46.54 and 80.82 % respectively. From the yield recorded with the test concentrations, 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, the concentration that inhibited 50% yield (EyC50) was calculated as 9.55 mg/L with upper confidence limit of 9.92 mg/L and lower confidence limit of 9.19 mg/L. The percent inhibition on the yield on day ‘7’ for the test concentrations of 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, was calculated as 036, 0.36, 23.23, 67.95, 79.17 and 95.92 % respectively. The NOEC was calculated as 2.2 mg/L and LOEC was calculated as 4.84 mg/L.
Frond Dry Weight:
From the average specific growth rate recorded with the test concentrations, 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, the concentration that inhibited 50% growth (ErC50) was calculated as 21.67 mg/L with upper confidence limit of 22.80 mg/L and lower confidence limit of 20.59 mg/L. The percent inhibition on the average growth rate on day ‘7’ for the concentration of 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L was calculated as 0.00, 0.43, 11.75, 35.04, 42.09 and 80.13 % respectively. From the yield recorded with the test concentrations, 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, the concentration that inhibited 50% yield (EyC50) was calculated as 8.69 mg/L with upper confidence limit of 9.04 mg/L and lower confidence limit of 8.35 mg/L.
The percent inhibition on the yield on day ‘7’ for the test concentration of 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, was calculated as 0.03, 1.44, 33.26, 70.94, 77.63 and 96.41 % respectively. The NOEC was calculated as 2.2 mg/L, while LOEC was 4.84 mg/L.
ErC50 of FAT 20290/E TE based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was found to be 21.12 mg/L.
EyC50 of FAT 20290/E TE based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was found to be 9.55 mg/L
ErC50 of FAT 20290/E TE based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was found to be 21.67 mg/L
EyC50 of FAT 20290/E TE based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was found to be 8.69 mg/L.
Reference
Description of key information
Lemna gibba (Duckweed) were exposed to the test item (FAT 20290/C TE) at different test concentrations viz 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L. Initially on day ‘0’, 12 fronds were inoculated in beaker containing 200 ml of test medium (20X-AAP medium). Three replicates for each test concentration and six replicates for control were maintained. The test item was formulated in 20X-AAP medium.
Prior to exposure, Lemna gibba was pre-cultured in the 20X-AAP medium for period of ‘8’ days and the pre-culture was found healthy.
After exposure, Lemna gibba was observed for frond count on days ‘3’, ‘5’ and ‘7’. Dry weight of the fronds were recorded at start (pre-culture representative samples) and at the end of the test.
Fronds were observed for their appearance on days ‘3’, ‘5’ and ‘7’. All fronds were found normal and healthy in all replicates of control and test concentrations except 51.54 mg/L where necrosis was observed on day 5 and in addition to necrosis, root filaments were turned blue in 10.65, 23.43 and 51.54 mg/L concentrations on day 7.
The doubling time (Td) of frond number in the control group was calculated as 1.62 day-1.
Frond Numbers:
From the average specific growth rate recorded with the different test concentrations viz 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, the test concentration that inhibited 50% growth (ErC50) was calculated as 21.12 mg/L with upper confidence limit of 22.14 mg/L and lower confidence limit of 20.14 mg/L.
The percent inhibition on the average specific growth rate on day ‘7’ for the test concentrations of 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, was calculated as 0.12, 0.11, 8.33, 34.62, 46.54 and 80.82 % respectively. From the yield recorded with the test concentrations, 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, the concentration that inhibited 50% yield (EyC50) was calculated as 9.55 mg/L with upper confidence limit of 9.92 mg/L and lower confidence limit of 9.19 mg/L. The percent inhibition on the yield on day ‘7’ for the test concentrations of 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, was calculated as 036, 0.36, 23.23, 67.95, 79.17 and 95.92 % respectively. The NOEC was calculated as 2.2 mg/L and LOEC was calculated as 4.84 mg/L.
Frond Dry Weight:
From the average specific growth rate recorded with the test concentrations, 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, the concentration that inhibited 50% growth (ErC50) was calculated as 21.67 mg/L with upper confidence limit of 22.80 mg/L and lower confidence limit of 20.59 mg/L.
The percent inhibition on the average growth rate on day ‘7’ for the concentration of 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L was calculated as 0.00, 0.43, 11.75, 35.04, 42.09 and 80.13 % respectively.
From the yield recorded with the test concentrations, 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, the concentration that inhibited 50% yield (EyC50) was calculated as 8.69 mg/L with upper confidence limit of 9.04 mg/L and lower confidence limit of 8.35 mg/L.
The percent inhibition on the yield on day ‘7’ for the test concentration of 1, 2.2, 4.84, 10.65, 23.43 and 51.54 mg/L, was calculated as 0.03, 1.44, 33.26, 70.94, 77.63 and 96.41 % respectively.
The NOEC was calculated as 2.2 mg/L, while LOEC was 4.84 mg/L.
ErC50of FAT 20290/E TE based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was found to be 21.12 mg/L.
EyC50of FAT 20290/E TE based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was found to be 9.55 mg/L
ErC50of FAT 20290/E TE based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was found to be 21.67 mg/L
EyC50of FAT 20290/E TE based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was found to be 8.69 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 21.12 mg/L
- EC10 or NOEC for freshwater plants:
- 2.2 mg/L
Additional information
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