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EC number: 249-682-3 | CAS number: 29529-99-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- The temperature reduction at day 29 occurred within the last hours of incubation and therefore is not considered to have an influence on the integrity of results at all.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione
- EC Number:
- 249-682-3
- EC Name:
- 6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione
- Cas Number:
- 29529-99-5
- Molecular formula:
- C11H20N4S2
- IUPAC Name:
- 6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- not specified
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- Prior to use a sample of activated sludge from the sewage plant at Taunusstein-Bleidenstadt was washed twice with mineral nutrient solution of the CO2-Evolution-Test to eliminate organic components and carbonates from the sludge. After resolution with mineral nutrient medium the sludge was aerated by means of compressed air for about four hours. Before use as inoculum for the CO2-Evolution-Test the sludge was homogenised in a "Waring Blender" at low speed for 2 minutes and then filtered through a cotton filter previously carefully rinsed with deionised water. The filtrate was used as inoculum (1% of the final volume of the test solution) on the same day of preparation
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- ca. 10 other: mg C/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The test item and polyvalent inoculum (sewage microorganisms from a sewage plant working with predominantly domestic sewage, see below) were incubated at room temperature for 28 days. CO2 released from the test item was trapped as BaCO3 using a trap system described in the indicated guideline and the study plan. The degradation was followed by CO2 analysis. After reference to blank control, the total amount of CO2 produced by the test item was determined for the test period and calculated as a percentage of the total CO2 that the test item, based on its carbon content, could have theoreti¬cally produced (%TCO2).
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 9
- St. dev.:
- 1.4
- Sampling time:
- 28 d
Any other information on results incl. tables
Results of Testing Biodegradability according to OECD 301 B
- 1stTest Solution with the Test Item -
Test Item: 6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione
Concentration: 73.0 mg / 3.5 L /»10 mg TOC1)/L
ThCO22): 1758.9 mg CO2/g test item
Theoretical amount of CO2being generated if the test item is completely transformed to CO2: 128.4 mg CO2/ 3500 mL test solution
Inoculum: Activated sludge from the sewage plant of Taunusstein-Bleidenstadt dealing with predominantly domestic sewage
Volume of the test solution: 3500 mL
Functional control: Sodium benzoate, 123.8 mg / 3.5 L test solution; equivalent to 263.1 mg CO2, 86 % biodegradation within 28 d (+ 1 d of purging off the CO2from the test solution)
Total CO2Evolution of the Blank: 83.9 mg CO2/ 3.5 L within 28 d (+ 1 d of purging off the CO2from the test solution)
Time |
Concentration: |
73.0 |
mg / 3.5 L ThCO2: |
128.4 |
mg CO2/ 3.5 L |
|
mg CO2produced in the Test solution, cumulative |
% TCO2 |
|||||
3 |
1.24 |
1 |
|
|||
6 |
2.26 |
2 |
|
|||
9 |
3.31 |
3 |
|
|||
14 |
3.31 |
3 |
|
|||
17 |
5.31 |
4 |
|
|||
22 |
8.85 |
7 |
|
|||
28 |
11.03 |
9 |
4) |
|||
29 |
|
12.48 |
|
10 |
5) |
|
1)TOC: Total Organic Carbon
2)ThCO2: Theoretical amount of CO2that can be generated by the test item
3)% TCO2: Percentage of total CO2the test item has generated in relation to the ThCO2
4) The test solution was stopped by the addition of 1 mL conc. HCl;
5) The difference between this titration and that before is due to the liberation of CO2after acidification of the test solution.
Table 2: Results of Testing Biodegradability according to OECD 301 B
- 2ndTest Solution with the Test Item -
Test Item: 6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione
Concentration: 73.0 mg / 3.5 L /»10 mg TOC1)/L
ThCO22): 1758.9 mg CO2/g test item
Theoretical amount of CO2being generated if the test item is completely transformed to CO2: 128.4 mg CO2/ 3500 mL test solution
Inoculum: Activated sludge from the sewage plant of Taunusstein-Bleidenstadt dealing with predominantly domestic sewage
Volume of the test solution: 3500 mL
Functional control: Sodium benzoate, 123.8 mg / 3.5 L test solution; equivalent to 263.1 mg CO2, 86 % biodegradation within 28 d (+ 1 d of purging off the CO2from the test solution)
Total CO2Evolution of the Blank: 83.9 mg CO2/ 3.5 L within 28 d (+ 1 d of purging off the CO2from the test solution)
Time |
Concentration: |
73.0 |
mg / 3.5 L ThCO2: |
128.4 |
mg CO2/ 3.5 L |
|
mg CO2produced in the Test solution, cumulative |
% TCO2 |
|||||
3 |
1.90 |
1 |
|
|||
6 |
3.06 |
2 |
|
|||
9 |
4.17 |
3 |
|
|||
14 |
4.17 |
3 |
|
|||
17 |
5.94 |
5 |
|
|||
22 |
8.57 |
7 |
|
|||
28 |
9.59 |
7 |
4) |
|||
29 |
|
10.47 |
|
8 |
5) |
|
1)TOC: Total Organic Carbon
2)ThCO2: Theoretical amount of CO2that can be generated by the test item
3)% TCO2: Percentage of total CO2the test item has generated in relation to the ThCO2
4) The test solution was stopped by the addition of 1 mL conc. HCl;
5) The difference between this titration and that before is due to the liberation of CO2after acidification of the test solution.
Table 3: Results of Testing Biodegradability according to OECD 301 B
- 3rdTest Solution, Sodium Benzoate-
Control Item: Sodiumbenzoate
Concentration:123.8 mg/3.5 L /»21 mg TOC1)/L
ThCO22): 2125.1 mg CO2/ g control item
Theoretical amount of CO2being generated if control item is completely transformed to CO2: 263.1 mg CO2/ 3500 mL test solution
Inoculum: Activated sludge from the sewage plant of Taunusstein-Bleidenstadt dealing with predominantly domestic sewage
Volume of the test solution: 3500 mL
Total CO2Evolution of the Blank: 83.9 mg CO2/ 3.5 L within 28 d (+ 1 d of purging off the CO2from the test solution)
Time |
Concentration: |
123.8 |
mg / 3.5 L ThCO2: |
263.1 |
mg CO2/ 3.5 L |
|
mg CO2produced in the Test solution, cumulative |
% TCO2 |
|||||
3 |
81.58 |
31 |
|
|||
6 |
162.49 |
62 |
|
|||
9 |
187.52 |
71 |
|
|||
14 |
200.31 |
76 |
|
|||
17 |
209.92 |
80 |
|
|||
22 |
217.64 |
83 |
|
|||
28 |
222.33 |
85 |
4) |
|||
29 |
226.87 |
|
86 |
5) |
||
1)TOC: Total Organic Carbon
2)ThCO2: Theoretical amount of CO2that can be generated by the control item
3)% TCO2: Percentage of total CO2the control item has generated in relation to the ThCO2
4) The test solution was stopped by the addition of 1 mL conc. HCl;
5) The difference between this titration and that before is due to the liberation of CO2after acidification of the test solution.
Table 4: Results of Testing Biodegradability according to OECD 301 B
- 4thTest Solution, Toxicity Control -
Item: 6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithioneand Sodium Benzoate,
Concentration of the test item: 73.0 mg / 3.5 L / ≈10 mg TOC1)/L
Concentration of sodiumbenzoate: 124.2 mg / 3.5 L / ≈21 mg TOC1)/L
Theoretical amount of CO2being generated if all the carbon material in the test solution is transformed to CO2: 392.3 mg CO2/ 3500 mL test solution
Inoculum: Activated sludge from the sewage plant of Taunusstein-Bleidenstadt dealing with predominantly domestic sewage
Volume of the test solution: 3500 mL
Sodiumbenzoate (separate
test solution): 123.8 mg/3.5 L test solution; equivalent to 263.1 mg CO2, 86 % biodegradation within 28 d (+ 1 d of purging off the CO2from the test solution)
Test item (separate test solutions): 73.0 mg/3.5 L test solution; equivalent to 128.4 mg CO2, 10 % biodegradation within 28 d (+ 1 d of purging off the CO2from the test solution)
73.0 mg/3.5 L test solution; equivalent to 128.4 mg CO2, 8% biodegradation within 28 d (+ 1 d of purging off the CO2from the test solution)
Total CO2Evolution of the Blank: 83.9 mg CO2/ 3.5 L within 28 d (+ 1 d of purging off the CO2from the test solution)
Time |
Concentration: |
124.2 |
mg Sodium Benzoate + |
73.0 |
mg Test Item |
|||
|
ThCO2: |
392.3 |
mg CO2/ 3.5 L |
|||||
mg CO2produced in the Test solution, cumulative |
% TCO2 |
|||||||
3 |
82.01 |
21 |
|
|||||
6 |
159.73 |
41 |
|
|||||
9 |
198.09 |
50 |
|
|||||
14 |
208.87 |
53 |
|
|||||
17 |
214.69 |
55 |
|
|||||
22 |
220.78 |
56 |
|
|||||
28 |
225.61 |
58 |
4) |
|||||
29 |
|
229.79 |
|
59 |
5) |
|||
1)TOC: Total Organic Carbon
2)ThCO2: Theoretical amount of CO2that can be generated by the test item + control item
3)% TCO2: Percentage of total CO2the test + control items have generated in relation to the ThCO2
4) The test solution was stopped by the addition of 1 mL conc. HCl;
5) The difference between this titration and that before is due to the liberation of CO2after acidification of the test solution.
From these degradation results it is assumed, that there is no toxicity towards the microorganisms (CO2-evolution is much higher than within the sodium benzoate control). |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- no
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item 6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione must be regarded as “not readily biodegradable"
- Executive summary:
6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione was tested for biodegradability according to 'CO2-Evolution-Test' (OECD Guideline 301B). Calculated from the organic carbon content of the test item and the measured CO2generation, 10 % of the theoretical CO2(ThCO2) has been generated by the test item within 28d of test period in the first culture with 73.0 mg of test item. 8% of the theoretical CO2(ThCO2) has been generated by the test item within 28d in the second culture with 73.0 mg of test item. The calculated mean degradation value of the test item was 9%.Thus, the test item6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithionemust be regarded as “not readily biodegradable". The control item sodium benzoate was degraded 86% and the conditions of the "10-days-window" were met within 6 days (62%). The total CO2-evolution of the Blank was 83.9 mg CO2/3.5L and thus being in the requested range of <40 mg/L as indicated in the OECD guideline 301B. Thus the test is regarded to be valid. The results obtained with the toxicity control indicate that there was no toxicity of the test item towards microorganisms at the concentration used within the test as a degradation value of >30% was achieved in total within that treatment (59%) within 28d (+1d).
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