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EC number: 249-596-6 | CAS number: 29385-43-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
Key values for assessment come from ISO 8192 study with activated sludge exposed to 1H-Benzotriazole.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 060 mg/L
- EC10 or NOEC for microorganisms:
- 394 mg/L
Additional information
In addition to the selected key study according to ISO 8192 several studies with Benzotriazole, Tolyltriazole as well as different commercially available aircraft deicing fluids (ADF) using Microtox test system (Vibrio fischeri) are available (Cancilla, 1997; Cancilla, 2003; Cornell, 2000; Pillard, 2001). The results are summarized below.
Compound |
Microtox EC50 (15 min) [mg/L] |
Study |
5-methylbenzotriazole |
4.25 (95% CI 4.18-4.35) |
Cancilla, 2003 |
4-methylbenzotriazole |
21 (95% CI 9.0-47) |
Pillard, 2001 |
5-methylbenzotriazole |
8.7 (95% CI 8.2-9.2) |
|
1:1 mixture of 4-MBT and 5-MBT |
7.3 (95% CI 6.9-7.7) |
|
1H-Benzotriazole |
41.65 ± 11.01 |
Cancilla, 1997 |
5-methylbenzotriazole |
5.91 ± 1.11 |
|
different ADFs with tolyltriazole |
6-9 |
Cornell, 2000 |
Based on these observations the three triazoles can be sorted by decreasing toxicity to Vibrio fischeri (Benzotriazole < 4-methylbenzotriazole < 5-methylbenzotriazole) showing a moderate toxicity, in general. However, since the Microtox test is a single-species test only the ISO study has been rated to be more relevant and accordingly selected as key study for the further assessment.
Similar findings to the effect concentrations in the key study with 1H-Benzotriazole has been derived from experimental observations from Gruden et al. (Gruden, 2001) exposing activated sludge with Tolyltriazole. In the toxicity assays a first significant decrease in methane production has been observed at 300 mg/L Tolyltriazole and an inhibition of methanogenic activity of about 50 % at 1000 mg/L Tolyltriazole indicating a likewise toxicity compared to the results of the testing with Benzotriazole.
Cancilla DA et al. (1997) Isolation and characterization of Microtox-active components from aircraft de-icing/anti-icing fluids, Environ Toxicol Chem, 16, 3, 430-434.
Cancilla DA et al. (2003) Studies of the environmental fate and effect of aircraft deicing fluids: Detection of 5-methyl-1H-benzotriazole in the fathead minnow (Pimphales Promelas), Environ Toxicol Chem, 22, 1, 134-140.
Gruden CL et al. (2001) Fate and Toxicity of Aircraft Deicing Fluid Additives Through Anaerobic Digestion, Water Environ Res, 73, 1, 72 -79.
Cornell JS et al. (2000) Comparative measures of the toxicity of component chemicals in aircraft deicing fluid, Environ Toxicol Chem, 19, 6, 1465-1472.
Pillard DA et al. (2001) Toxicity of Benzotriazole and Benzotriazole derivatives to three aquatic species, Wat Res, 35, 2, 557-560.
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