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EC number: 244-469-1 | CAS number: 21598-22-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2018-12-11 to 2018-12-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13th April 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 30 May 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Version / remarks:
- October 2016
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Batch No.of test material: Lab NP_20171034-003
- Expiration date of the lot/batch: 2022-07-24
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
OTHER SPECIFICS:
white solid - Analytical monitoring:
- yes
- Details on sampling:
- Four parallel samples were taken from the 100 mg/L concentration level and from the control at the start and end of the experiment.
- Vehicle:
- no
- Details on test solutions:
- The test solution used in the test was prepared by mechanical dispersion. 0.0700 g of test item were dissolved in 700 mL dilution water (ISO Medium) in order to obtain the concentration of 100 mg/L.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Waterflea
- Strain/clone: Straus
- Source: Laboratory of Hydrobiology (Central Agricultural Office, Directorate of Plant-, and Soil Protection) 2100 Gödöllő, Kotlán S. u. 3. Hungary
- Age at test start: Less than 24 hours old
- Feeding during test: no
ACCLIMATION
- Acclimation period: no, breeding conditions same as test conditions - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 249 mg/L (as CaCO3)
- Test temperature:
- 18.4 – 21.5°C
- pH:
- 7.69 – 7.82
- Dissolved oxygen:
- 7.19 – 7.64 mg/L
- Nominal and measured concentrations:
- nominal concentration: 100 mg/L
The exposure concentration of the saturated test solution was analytically determined to be 5.84 mg/L (calculated as the geometric mean of start and end concentrations). - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers
- Material, fill volume: glass, 40 mL medium
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: ISO medium, according to OECD 202
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light (artificial illumination) and 8 hours darkness
- Light intensity: 780 lux
EFFECT PARAMETERS MEASURED
after 24 and 48 hours of exposure visual observation (immobility)
RANGE-FINDING STUDY
- Test concentrations: 100 mg/L nominal concentration
- Results used to determine the conditions for the definitive study: No immobility or any sub-lethal effect was detected in the test concentration and in the control group during the 48-h exposure period. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 5.84 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 5.84 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Behavioural abnormalities:
No
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this 48-hour static acute toxicity test with Daphnia magna the test item had no toxic effect on Daphnia at aquatic saturation (measured value: 5.84 mg/L; equivalent to 100 mg/L nominal concentration). Accordingly, the 48-h EC50 value was determined to be > 5.84 mg/L (equivalent to 100 mg/L nominal concentration). The 48-h NOEC was determined to be 5.84 mg/L (equivalent to 100 mg/L nominal concentration).
- Executive summary:
The purpose of this study was to evaluate the influence of the test item on the mobility respectively survival of Daphnia magna. A limit test was performed in which young Daphnia were exposed to aqueous test media containing the test item for 48 hours at the limit of its solubility in the test medium (i.e. saturation) plus a control in order to demonstrate that the test item has no influence on the mobility of Daphnia up to at least the saturation concentration.Limit test; using a single concentration at saturation (corresponding to 100 mg/L nominal concentration); concurrent control ran.The exposure concentration of the saturated test solution was analytically determined to be 5.84 mg/L (calculated as the geometric mean of start and end concentrations).The test item and the control were tested using 20 Daphnia, divided into four groups (glass beaker; test medium app. 40 mL) of five animals each were exposed to the test item concentration or run as control for 48 hours under static conditions. The immobility or mortality of the Daphnia was determined by visual observation after 24 and 48 hours of exposure.Concentrations of the test item were determined at the start (0 hour) and the end of exposure (48 hours) using ICP-OES method.Statistical analysis was not necessary; endpoints were determined directly from the raw data. All validity criteria were met and therefore the study is considered as valid. The mean measured concentration of the test item was 6.22 mg/L at the start and 5.49 mg/L at the end of the test. There was no immobilisation observed in twenty daphnids exposed to each test item treated and control group. Further no abnormal behaviour or appearance of test animals was noted. In this 48-hour static acute toxicity test with Daphnia magna the test item had no toxic effect on Daphnia at aquatic saturation (measured value: 5.84 mg/L; equivalent to 100 mg/L nominal concentration). Accordingly, the 48-h EC50 value was determined to be > 5.84 mg/L (equivalent to 100 mg/L nominal concentration). The 48-h NOEC was determined to be 5.84 mg/L (equivalent to 100 mg/L nominal concentration).
Reference
Description of key information
In a 48-hour static acute toxicity test with Daphnia magna the test item had no toxic effect on Daphnia at aquatic saturation (measured value: 5.84 mg/L; equivalent to 100 mg/L nominal concentration). Accordingly, the 48-h EC50 value was determined to be > 5.84 mg/L (equivalent to 100 mg/L nominal concentration). The 48-h NOEC was determined to be 5.84 mg/L (equivalent to 100 mg/L nominal concentration).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 5.84 mg/L
Additional information
A study according OECD TG 202 was conducted to evaluate the influence of the test item on the mobility respectively survival of Daphnia magna. A limit test was performed in which young Daphnia were exposed to aqueous test media containing the test item for 48 hours at the limit of its solubility in the test medium (i.e. saturation) plus a control in order to demonstrate that the test item has no influence on the mobility of Daphnia up to at least the saturation concentration. Limit test; using a single concentration at saturation (corresponding to 100 mg/L nominal concentration); concurrent control ran. The exposure concentration of the saturated test solution was analytically determined to be 5.84 mg/L (calculated as the geometric mean of start and end concentrations). In this 48-hour static acute toxicity test with Daphnia magna the test item had no toxic effect on Daphnia at aquatic saturation (measured value: 5.84 mg/L; equivalent to 100 mg/L nominal concentration). Accordingly, the 48-h EC50 value was determined to be > 5.84 mg/L (equivalent to 100 mg/L nominal concentration). The 48-h NOEC was determined to be 5.84 mg/L (equivalent to 100 mg/L nominal concentration).
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