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Diss Factsheets
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EC number: 240-162-1 | CAS number: 16024-58-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October - November 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- Adopted: 04. April 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- Richtlinie der Kommission vom 25. April 1984
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling method: With a bottle 100 mL were sampled in the middle of the tank
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The test substance was diluted with dilution water, homogenised with a ultrasonic bath and Ultra-Turrax. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Strain: HAMILTON-BUCHANAN
- Source: West Aquarium, 3422 Bad Lauterberg
- Length at study initiation: range: 2.8 - 3.5 cm; mean: 3.2 cm
- Weight at study initiation: not available
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test conditions
- Type and amount of food during acclimation: Tetra Min, Tetra Werke, Melle
- Feeding frequency during acclimation: twice a day
- Health during acclimation: no mortality observed - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- 22.0 - 22.6 °C
- pH:
- 7.7 - 8.3
- Dissolved oxygen:
- 7.0 - 9.1 mg/L
- Nominal and measured concentrations:
- Nominal concentration: 500 mg/L
Measured concentration: 508.3 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass tank in water bath
- Material, fill volume, size: glass, 10L, 30cm x 22cm x 24cm
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: < 1 g fish/L water
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: Following ISO/DIS 7346/1
OTHER TEST CONDITIONS
- Adjustment of pH: yes, with 10M NaOH
- Photoperiod: 12 h light, 12 h dark
- Light intensity: ca 700 Lux
EFFECT PARAMETERS MEASURED
Mortality, ability to swim, breathing and water quality paramers were recorded after 2-4, 24, 48, 72 and 96 hours.
Pretest: yes, but no details availabe - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: no
- Observations on body length and weight: no abnormalities
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Sublethal observations / clinical signs:
Sampling date
1990-11-26
1990-11-28
1990 -11-30
Geometric mean measured concentration [mg/L]
Nominal concentration
Meas. conc. [mg/L]
%
Meas. conc. [mg/L]
%
Meas. conc. [mg/L]
%
Control
<1.0
<1.0
<1.0
500 mg/L
504
100.8
489
97.8
532
106.4
508.3
Meas. conc. = Measured concentration of the test item, enrichment and dilution factor taken into account
% = Percent of nominal concentration of test item
Geometric mean measured test item concentrations [mg/L]
Observation*
Number of fish effected after different exposure periods [hours]
3
24
48
72
96
500
N
7
7
7
7
7
Control
N
7
7
7
7
7
*The letter correspond to the following observations:
N = Normal behavior
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study the test item was found to cause no lethal effects to zebrafish after 96 hours at a concentration of 500 mg test item/L. The LC50 after 96 hours was > 500 mg test item/L.
- Executive summary:
The acute toxicity of the test item to fish (zebrafish) was determined according to the principles of the OECD-Guideline for Testing of Chemicals No. 203 (1984) and EG Guideline C.1 (1984) in 1990. An acute toxicity test under static conditions was conducted with the nominal test item concentration of 500.0 mg/L. Duration of the test was 96 hours. Ten test organisms were exposed to the test concentration and the control. Water quality parameters (temperature, pH-value and oxygen- saturation) measured at 2 -4, 24, 48, 72 and 96 hours were determined to be within the acceptable limits. The determination of the test concentrations was carried out via HPLC (UV). Evaluation was carried out from the media after 0, 48 and 96 h. The measured concentration was 489 mg/L, less than 20% below the nominal concentration. Therefore, all effect values are based on nominal concentrations. All validity criteria of the test guideline were met. The LC50 was determined to be > 500 mg/L.
Reference
Description of key information
The test item was found to cause no lethal effects to zebrafish after 96 hours at a concentration of 500 mg test item/L. The LC50 after 96 hours was > 500 mg test item/L.
Key value for chemical safety assessment
Additional information
The acute toxicity of the test item to fish (zebrafish) was determined according to the principles of the OECD-Guideline for Testing of Chemicals No. 203 (1984) and EG Guideline C.1 (1984) in 1990. An acute toxicity test under static conditions was conducted with the nominal test item concentration of 500.0 mg/L. Duration of the test was 96 hours. Ten test organisms were exposed to the test concentration and the control. Water quality parameters (temperature, pH-value and oxygen- saturation) measured at 2 -4, 24, 48, 72 and 96 hours were determined to be within the acceptable limits. The determination of the test concentrations was carried out via HPLC (UV). Evaluation was carried out from the media after 0, 48 and 96 h. The measured concentration was 489 mg/L, less than 20% below the nominal concentration. Therefore, all effect values are based on nominal concentrations. All validity criteria of the test guideline were met. The LC50 was determined to be > 500 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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