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EC number: 236-039-7 | CAS number: 13114-72-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10.04.-23.04.2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- yes
- Remarks:
- Principal investigator for analytical determination of the test substance concentrations was not Pavla Vlasáková, Dipl.Eng. In this study principal investigator was Jana Netušilová, Dipl.Eng., CETA, GLP Test Facility, Analytical Group I.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The samples of test solutions were prepared and delivered by Ecotoxicity group. The samples were analyzed on the day of delivery. The samples with nominal concentration 1 mg/L were analysed non-diluted. The samples with nominal concentration 100 mg/L were appropriate diluted (25 times) with UPW to the range of calibration curve. All samples were filtered through the syringe PTFE filter 0.45 μm before HPLC analyses.
- Details on test solutions:
- The stock solution of the test substance was prepared in the test medium. 100 mg of the test substance was weighed into 1000 mL of the test medium for preliminary and limit test. Because the test substance was poorly soluble for dilution was used ultrasound for 1 hours and stirring for 2 hours.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM- Common name: Desmodesmus subspicatus - Source (laboratory, culture collection): Brinkmann 1953/SAG 86.81 obtained from the collection of autotrophic organisms of The Botanic Institute of the Czech Academy of Science, Třeboň on date 3.6.2014. - Age of inoculum (at test initiation): Inoculum culture was kept 3-4 days under conditions at which the test was performed.- Method of cultivation: The strain culture was preinoculated from the stock solution and cultivated in flasks with the test medium on indirect daylight at laboratory temperature. ACCLIMATION- Acclimation period: 3-4 days- Culturing media and conditions: same as test
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 23.5-24°C
- pH:
- 7.6-7.8
- Nominal and measured concentrations:
- 1, 5, 10, 50, 100 mg/L preliminary test100 mg/L limit test
- Details on test conditions:
- TEST SYSTEM- Test vessel: Erlenmayer flask with stoppers venting air- Material, size, headspace, fill volume: glass, 50 ml- Aeration: without- Initial cells density: 5000 cells in 1 ml- Control end cells density:-No. of vessels per concentration (replicates): 3OTHER TEST CONDITIONSTemperature: 21 - 24 °C controlled at ± 2 °CLighting: under continuous white light, 4 440 lux to 8 880 luxExposition time: 72 hoursVolume of tested mixture: 50 mLInitial concentration of algae culture: 5 000 cells in a 1 mL Without aeration.Agitation of algae culture by shaking.The light intensity and temperature were measured every 24 hours. The density of algae culture was evaluated microscopically at 24, 48 and 72 hour.The cell density was measured by direct counting of living cells in Burker´s counting chamber.
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- other: EyC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: NOECr
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- other: NOECy
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Results with reference substance (positive control):
- Results of reference testThe sensitivity of the test species and correctness of test performance in our laboratory is periodically verified on a six-month period by testing the reference substance, potassium dichromate.The results of the verification test with K2Cr2O7, carried out in period from 30.3. to 2.4. 2015, are the following: 72 hour – ErC50 = 0.79 mg.L-1 (95% confidence limit: 0.70 – 0.89 mg.L-1)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The highest inhibition of growth rate was 14.5 % and the highest inhibition of yield was47.8 % in the limit test.Therefore exact values of EC50 could not be calculated and the values of EC are givenin the form of a range.The determination of NOEC values was done by ANOVA (Analysis of Variance) analysis. Used ANOVA method is the part of statistical software QC.Expert 2.5 © 1998-2000 (product of TriloByte Ltd., Czech Republic). There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 per cent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values. So the nominal concentrations were used for all evaluations and results.
- Executive summary:
The test substance,Akardit, was tested for growth inhibition on algae Desmodesmus subspicatus.
The test was performed according tomethod C.3. -Freshwater Algae and Cyanobacteria, Growth Inhibition Test, Commission Regulation (EC) No. 761/2009, Annex IV.
The preliminary test was performed in range of test substance nominal concentrations 1 – 100 mg·L-1.
The analytical results showed, that the test substance Akardit was sufficiently stable in the test medium at conditions of the test in concentration range 1 – 100 mg·L-1.
The highest inhibition of growth rate was 14.9 % and the highest inhibition of yield was
49.4 % in the preliminary test.Based on no toxicity of the test substance found in preliminary test, the limit test was performed subsequently.The concentration of 100 mg·L-1was tested in the limit test.The highest inhibition of growth rate was 14.5 % and the highest inhibition of yield was 47.8 % in the limit test.
The nominal concentrations were used for all evaluations and results, because, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 per cent of the nominal or measured initial concentration throughout the test, then the results can be based on nominal or measured initial values.
Test results:
72 hour – ErC50>100 mg·L-1 (nominal concentration)
72 hour – EyC50>100 mg·L-1 (nominal concentration)
72 hour – NOECr< 100 mg·L-1 (nominal concentration)
72 hour – NOECy< 100 mg·L-1 (nominal concentration)
Reference
Table No.: 1. Individual cell densities– preliminary test
Nominal concentration mg·L-1 | Replicates | Cell density in 1 mL | ||||
0 hours | 24 hours | 48 hours | 72 hours | |||
100 | 1 | 5000 | 6250 | 12500 | 206250 | |
2 | 5000 | 6250 | 12500 | 243750 | ||
3 | 5000 | 6250 | 18750 | 231250 | ||
50 | 1 | 5000 | 6250 | 25000 | 231250 | |
2 | 5000 | 6250 | 31250 | 268750 | ||
3 | 5000 | 6250 | 25000 | 243750 | ||
10 | 1 | 5000 | 6250 | 37500 | 300000 | |
2 | 5000 | 6250 | 43750 | 300000 | ||
3 | 5000 | 6250 | 31250 | 368750 | ||
5 | 1 | 5000 | 6250 | 43750 | 350000 | |
2 | 5000 | 6250 | 50000 | 362500 | ||
3 | 5000 | 12500 | 43750 | 362500 | ||
1 | 1 | 5000 | 12500 | 56250 | 375000 | |
2 | 5000 | 12500 | 62500 | 393750 | ||
3 | 5000 | 12500 | 62500 | 375000 | ||
Control | 1 | 5000 | 18750 | 87500 | 437500 | |
2 | 5000 | 18750 | 81250 | 443750 | ||
3 | 5000 | 18750 | 75000 | 450000 |
Table No.: 2. Growth rate (µ), yield (Y) – preliminary test
Nominal concentration mg·L-1 | Replicates | Growth ratefor section-by-section (m) | Growth rate (m) 0-72 h | Yield (Y) | ||
0-24 h | 24-48 h | 48-72 h | ||||
100 | 1 | 0.22 | 0.69 | 2.80 | 1.24 | 201250 |
2 | 0.22 | 0.69 | 2.97 | 1.30 | 238750 | |
3 | 0.22 | 1.10 | 2.51 | 1.28 | 226250 | |
50 | 1 | 0.22 | 1.39 | 2.22 | 1.28 | 226250 |
2 | 0.22 | 1.61 | 2.15 | 1.33 | 263750 | |
3 | 0.22 | 1.39 | 2.28 | 1.30 | 238750 | |
10 | 1 | 0.22 | 1.79 | 2.08 | 1.36 | 295000 |
2 | 0.22 | 1.95 | 1.93 | 1.36 | 295000 | |
3 | 0.22 | 1.61 | 2.47 | 1.43 | 363750 | |
5 | 1 | 0.22 | 1.95 | 2.08 | 1.42 | 345000 |
2 | 0.22 | 2.08 | 1.98 | 1.43 | 357500 | |
3 | 0.92 | 1.25 | 2.11 | 1.43 | 357500 | |
1 | 1 | 0.92 | 1.50 | 1.90 | 1.44 | 370000 |
2 | 0.92 | 1.61 | 1.84 | 1.46 | 388750 | |
3 | 0.92 | 1.61 | 1.79 | 1.44 | 370000 | |
Control | 1 | 1.32 | 1.54 | 1.61 | 1.49 | 432500 |
2 | 1.32 | 1.47 | 1.70 | 1.50 | 438750 | |
3 | 1.32 | 1.39 | 1.79 | 1.50 | 445000 |
The mean coefficient of variationfor section-by-section specific growth rates in the control cultures is3.5 %(CV0-24h= 0 %, CV24-48h= 5.3 % a CV48-72h= 5.4 %).
The coefficient of variation of average specific growth ratesduring the whole test period in replicate control cultures is0.3 %.
Table No.: 7 Individual cell densiities-limit test
Nominal concentration mg·L-1 | Replicates | Cell density in 1 mL | ||||
0 hours | 24 hours | 48 hours | 72 hours | |||
100 | 1 | 5000 | 12500 | 43750 | 200000 | |
2 | 5000 | 12500 | 50000 | 193750 | ||
3 | 5000 | 12500 | 43750 | 193750 | ||
4 | 5000 | 12500 | 56250 | 225000 | ||
5 | 5000 | 6250 | 56250 | 287500 | ||
6 | 5000 | 12500 | 43750 | 200000 | ||
Control | 1 | 5000 | 18750 | 81250 | 425000 | |
2 | 5000 | 18750 | 75000 | 400000 | ||
3 | 5000 | 12500 | 75000 | 418750 | ||
4 | 5000 | 25000 | 81250 | 393750 | ||
5 | 5000 | 18750 | 68750 | 418750 | ||
6 | 5000 | 12500 | 75000 | 406250 |
Table No.: 8. Growth rate (µ), yield (Y) – limit test
Nominal concentration mg·L-1 | Replicates | Growth rate for section-by-section (m) | Growth rate (m) 0-72 h | Yield (Y) | ||
0-24 h | 24-48 h | 48-72 h | ||||
100 | 1 | 0.92 | 1.25 | 1.52 | 1.23 | 195000 |
2 | 0.92 | 1.39 | 1.35 | 1.22 | 188750 | |
3 | 0.92 | 1.25 | 1.49 | 1.22 | 188750 | |
4 | 0.92 | 1.50 | 1.39 | 1.27 | 220000 | |
5 | 0.22 | 2.20 | 1.63 | 1.35 | 282500 | |
6 | 0.92 | 1.25 | 1.52 | 1.23 | 195000 | |
Control | 1 | 1.32 | 1.47 | 1.65 | 1.48 | 420000 |
2 | 1.32 | 1.39 | 1.67 | 1.46 | 395000 | |
3 | 0.92 | 1.79 | 1.72 | 1.48 | 413750 | |
4 | 1.61 | 1.18 | 1.58 | 1.46 | 388750 | |
5 | 1.32 | 1.30 | 1.81 | 1.48 | 413750 | |
6 | 0.92 | 1.79 | 1.69 | 1.47 | 401250 |
The mean coefficient of variation for section-by-section specific growth rates in the control cultures is 14.5% (CV0-24h= 21.9 %, CV24-48h= 17.2 % a CV48-72h= 4.5 %).
The coefficient of variation of average specific growth rates during the whole test period
in replicate control cultures is 0.7 %.
Description of key information
The test substance, Akardit, was tested for growth inhibition on algae Desmodesmus subspicatus.
The test was performed according tomethod C.3. -Freshwater Algae and Cyanobacteria, Growth Inhibition Test, Commission Regulation (EC) No. 761/2009, Annex IV.
Only one study is available.
GLP study.
Klimish score 1.
The concentration of 100 mg·L-1was tested in the limit test. The highest inhibition of growth rate was 14.5 % and the highest inhibition of yield was 47.8 % in the limit test.
72 hour – ErC50>100 mg·L-1 (nominal concentration)
72 hour – EyC50>100 mg·L-1 (nominal concentration)
72 hour – NOECr< 100 mg·L-1 (nominal concentration)
72 hour – NOECy< 100 mg·L-1 (nominal concentration)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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