Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 236-039-7 | CAS number: 13114-72-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13.04.-24.04.2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- yes
- Remarks:
- Principal investigator for analytical determination of the test substance concentrations was not Pavla Vlasáková, Dipl.Eng. In this study principal investigator was Jana Netušilová, Dipl.Eng., CETA, GLP Test Facility, Analytical Group I.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100, 50, 10, 5, 1 mg/L (measured at 0, 24, 48 and 96h)
- Vehicle:
- no
- Details on test solutions:
- The stock solution of the test substance was prepared in the dilution water. 100 mg of the test substance was weighed into 1000 mL of the dilution water for the preliminary test. Because the test substance was poorly soluble for dilution was used ultrasound for 1 hours and stirring for 2 hours. The concentrations of solutions used in the preliminary test were obtained by dilution of the stock solution with dilution water.
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- TEST ORGANISM- Common name: Poecilia reticulata - Source: certified supplier EMPLA s.r.o., Hradec Králové (Czech Republic) - Age at study initiation: 3-4 months- Length at study initiation: 2.0-3.0 cm- Method of breeding: in all-glass aquaria in aerated drinking water.- Feeding during test: without feedingACCLIMATION- Acclimation period: 7 days in dilution water- Acclimation conditions: same as test- Feeding during test: without feeding- Food type: “Tropica” - feed for aquarium fish (manufacturer: DajanaPET, s.r.o., Bohuňovice- Frequency: once a day- Health during acclimation (any mortality observed): mortality control, The sensitivity of test species is periodically monitored by testing with the reference substance - potassium dichromate.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- Ca + Mg ions 2.5 mmol.L-1
- Test temperature:
- 22±2 °C
- pH:
- 7.8±0.2
- Dissolved oxygen:
- air saturation value at 22 °C is 8.74
- Nominal and measured concentrations:
- 100, 50, 10, 5, 1 mg/L
- Details on test conditions:
- TEST SYSTEM- Test vessel: glass beakers, fill volume 1000 ml- Type: open- Aeration: no- Renewal rate of test solution (frequency/flow rate): 24h- No. of organisms per vessel: 7 fishes for each concentration- No. of vessels per concentration (replicates): 1- No. of vessels per control (replicates): 1- Biomass loading rate: 1 g/lDILUTION WATERStandard dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D in 1000 mL of deionized water.A.117.6 g CaCl2.2H20 in 1L deionized waterB.49.3 g MgSO4.7H2O in 1L deionized waterC.25.9 g NaHCO3 in 1L deionized waterD.2.3 g KCl in 1L deionized waterThe dilution water should be of pH 7.8 ± 0.2, value measured in this study was 7.8. It is saturated by oxygen by the aeration at temperature of 20 – 24 °C. The content of sum Ca + Mg ions should have the value of 2.5 mmol.L-1, value measured in this study was 2.5 mmol.L-1OTHER TEST CONDITIONS- Photoperiod: natural variation of daily light and darkEFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The fish mortality was observed each 24 hours. The pH, temperature and dissolved oxygen were measured at the same intervals.
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- See table No.: 1
- Results with reference substance (positive control):
- The sensitivity of the test species and correctness of test performance is periodically verified in six-month period by testing the reference substance, potassium dichromate.The results of verification test with K2Cr2O7 carried out in period from 13.04. to 17.04. 2015. are the following:48 hour – LC50 = 180 mg.L-1 (95% confidence limit: 153 – 206 mg.L-1)96 hour – LC50 = 165 mg.L-1 (95% confidence limit: 137 – 192 mg.L-1)
- Sublethal observations / clinical signs:
Table No.: 1 Fish mortality – preliminary test
Nominal concentration
mg·L-1
100
50
10
5
1
C
Number of live fish
2 hours
7
7
7
7
7
7
Mortality %
2 hours
0
0
0
0
0
0
Number of live fish
24 hours
7
7
7
7
7
7
Mortality %
24 hours
0
0
0
0
0
0
Number of live fish
48 hours
7
7
7
7
7
7
Mortality %
48 hours
0
0
0
0
0
0
Number of live fish
72 hours
7
7
7
7
7
7
Mortality %
72 hours
0
0
0
0
0
0
Number of live fish
96 hours
7
7
7
7
7
7
Mortality %
96 hours
0
0
0
0
0
0
- Validity criteria fulfilled:
- yes
- Conclusions:
- No mortality was observed in the preliminary test. The exact values of LC50 could not be calculated and the values of LC are given in the form of a range. The LC100 and LC0 values were identified by direct observation.Based on no toxicity of the test substance found in preliminary test, the study was terminated. The experiment with the concentration of 100 mg•l-1 included in the preliminary test has been performed using 7 fish, therethrough the conditions of the limit test have been fullfiled. It was demonstrated that the LC50 is higher than this concentration.The analytical results from the preliminary test showed, that the test substance Akardit was stable in dilution water at conditions of the test, therefore the nominal concentrations were used for all evaluations and results.
- Executive summary:
The test substance, Akardit, was tested for acute toxicity on fish Poecilia reticulata.
The test was performed according to method C.1 – Acute toxicity for fish, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
The preliminary test was performed in range of test substance nominal concentrations
1 – 100 mg·L-1. Based on no toxicity of the test substance found in preliminary test,
the study was terminated.The experiment with the concentration of 100 mg·l-1included
in the preliminary test has been performed using 7 fish, therethrough the conditions
of the limit test have been fullfiled. It was demonstrated that the LC50is higher than this concentration.Samples for analytical determination were taken at the beginning and at the end of the test. The analytical results from the preliminary test showed, that the test substance
Akarditwas stable in dilution water at conditions of the test, therefore the nominal concentrations were used for all evaluations and results.The test was performed as static.
Test results:
24 hour – LC50>100 mg·L-1 (nominal concentration)
48 hour – LC50>100 mg·L-1 (nominal concentration)
72 hour – LC50>100 mg·L-1 (nominal concentration)
96 hour – LC50>100 mg·L-1 (nominal concentration)
24 hour – LC0=100 mg·L-1 (nominal concentration)
48 hour – LC0=100 mg·L-1 (nominal concentration)
72 hour – LC0=100mg·L-1 (nominal concentration)
96 hour – LC0=100mg·L-1 (nominal concentration)
24 hour – LC100>100 mg·L-1 (nominal concentration)
48 hour – LC100>100 mg·L-1 (nominal concentration)
72 hour – LC100>100 mg·L-1 (nominal concentration)
96 hour – LC100>100 mg·L-1 (nominal concentration
Reference
Description of key information
Akardit was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method C.1 – Acute toxicity for fish, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
Only one study is available.
GLP study.
Klimish score 1.
The analytical results from the preliminary test showed, that the test substance Akardit was stable in dilution water at conditions of the test, therefore the nominal concentrations were used for all evaluations and results.
96 hour – LC50>100 mg·L-1Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.