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EC number: 207-623-9 | CAS number: 485-72-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 March - 20 November 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not specified
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Lot No. of test material: 07-170-FIL-2/3
- Appearance: White powder
- Expiration date: May 2010
- Purity: 98.4%
- Storage condition of test material: Ambient - Analytical monitoring:
- yes
- Details on sampling:
- The concentration of test substance was determined in each test solution at 0 and 48 hours by standard analytical methods that were validated prior to dosing (not described in the report).
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test material was determined to be insoluble in water. Therefore, a water-soluble fraction was prepared by adding the required nominal amount of test substance in moderately hard synthetic freshwater and mixing the solution for 20-24 hours. After this mixing time, the undissolved test substance was separated from the solution and only this water-soluble fraction was used for testing.
- Controls: Untreated control and solvent control
- Chemical name of vehicle: Dimethyl formamide (DMF)
- Concentration of vehicle in test medium: DMF was added as a solvent to the water-soluble fraction at 2.0 mL per 2 L dilution water. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: STILLMEADOW, Inc. culture laboratory
- Age: No greater than 24 hours old at dosing
- Feeding during test: No
ACCLIMATION
Before dosing, the daphnids were held in water of the quality used in the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- Range-finding: 100 mg/L CaCO3
Definitive: 85 mg/L CaCO3 - Test temperature:
- 20 ± 2°C (actual temperature in definitive test: 21 - 22°C)
- pH:
- Range-finding: pH 8.4
Definitive: measured pH
- 0 hours: 7.7 - 7.8
- 48 hours: 7.2 - 7.6 - Dissolved oxygen:
- 60 - 105% saturation at dosing
Measured dissolved oxygen:
- 0 hours: 9.0 - 9.9 mg/L
- 48 hours: 7.9 - 8.5 mg/L - Salinity:
- No data
- Conductivity:
- Definitive test (measured):
- 0 hours: 280 - 290 µmhos/cm
- 48 hours: 295 - 300 µmhos/cm - Nominal and measured concentrations:
- Nominal concentrations: 100 and 1000 mg/L
Measured: See table 1 - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers
- Material, size, headspace, fill volume: 250 mL glass beakers; filled with 200 mL of the appropriate solution
- Aeration: No
- No. of organisms per vessel: 10 daphnids per vessel
- No. of vessels per concentration: 3 replicates per concentration
- No. of vessels per control: one control group, 3 replicates
- No. of vessels per vehicle control (replicates): one solvent control group (1.0 mL DMF in 1000 mL dilution water added to the stock solution), 3 replicates
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Moderately hard laboratory freshwater
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light/ 8 hours dark cycle
EFFECT PARAMETERS MEASURED: At 24 and 48 hours following dosing, each beaker was examined for immobility and the number of live daphnids was recorded.
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY
- Test concentrations: 1.0, 10, 50, 100, 500 and 1000 mg/L
- Results used to determine the conditions for the definitive study: Immobility was less than 10%. - Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mobility: A mobility rate of 100% was observed in organisms treated with 100 mg/L and a 73% mobility rate was observed in organisms treated with 1000 mg/L.
- Mobility of control: A mobility rate of 97% was observed in the control organisms.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: At 1000 mg/L, the test solutions were cloudy and after 24 hours, the water was too turbid to count. - Validity criteria fulfilled:
- yes
- Remarks:
- Both the range-finding and definitive test control immobility was less than 10%.
- Conclusions:
- The 48 hour EC50 of Formononetin was determined to be >1000 mg/L. The NOEC for survival was 100 mg/L.
Reference
Table 1: Mobility data
Nominal concentration |
Replicate |
Number of mobile organisms |
||
0 hours |
24 hours |
48 hours |
||
0 |
A |
10 |
10 |
9 |
B |
10 |
10 |
10 |
|
C |
10 |
10 |
10 |
|
100 |
A |
10 |
10 |
10 |
B |
10 |
10 |
10 |
|
C |
10 |
10 |
10 |
|
1000 |
A |
10a |
* |
8b |
B |
10a |
* |
7b |
|
C |
10a |
* |
7b |
|
Solvent control (DMF) |
A |
10 |
10 |
10 |
B |
10 |
10 |
10 |
|
C |
10 |
10 |
10 |
* - too turbid to count
a– water cloudy
b– organisms lethargic, water cloudy
Table 2: Dose verification
|
Control |
Solvent control DMF |
mg/L |
|
Time point |
NA |
NA |
100 |
1000 |
Day 0 |
0.53 |
0.57 |
1.03 |
4.96 |
48 hour |
0.54 |
NA |
0.60 |
2.06 |
Description of key information
A GLP study was performed in accordance with OECD Guideline 202, to determine the toxicity of the test substance, Formononetin, to the freshwater invertebrate Daphnia magna in a 48 hour static test. The 48 hour EC50 of Formononetin was determined to be >1000 mg/L and the NOEC for survival was 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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