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EC number: 202-095-6 | CAS number: 91-76-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Skin irritation was tested with rabbits and the substance is described as not irritating. Eye irritation was tested with rabbits and the substance is described as slightly irritating.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21.06.1988-24.06.1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was performed according to OECD guideline study with GLP compliance.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Version / remarks:
- adopted May 12, 1981
- GLP compliance:
- yes
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Broekman Institute, Someren, NL
- Age at study initiation: 4575-F (animal #1): 22 weeks, 4625-F (animal #2): 17 weeks, 4629-F (animal #3): 17 weeks
- Weight at study initiation: 4575-F: 3551 g, 4625-F: 3073 g, 4629-F: 2831g
- Housing: individual in plastic cage with perforated floor
- Diet (e.g. ad libitum): 100 g LKK-20, diameter: 4 mm
- Water (e.g. ad libitum): free access to tap water (via automatic nozzles)
- Acclimation period: five days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-21 °C
- Humidity (%): 55-75 %
- Air changes (per hr): no information available
- Photoperiod (hrs dark / hrs light): 12 h dark, 12 h light - Type of coverage:
- occlusive
- Preparation of test site:
- shaved
- Vehicle:
- water
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- 0.5 g of test substance were moistened with 0.3 mL Milli-RO water. Each portion of the test substance was spread on a 6 cm^2 patch of Metalline and mounted on permeable tape. This was applied to the left flank of each animal, the right flank was being covered without test substance.
- Duration of treatment / exposure:
- Animals were wrapped in flexible bandage for 4 hours after which period the remaining test substance was removed using dry tissue and subsequently a tissue moistened with tap-water.
- Observation period:
- 60 min, 24 h, 48 h, and 72 h after removal of the patches.
- Number of animals:
- three female rabbits
- Details on study design:
- TEST SITE
- Area of exposure: 6 cm^2
- % coverage:
- Type of wrap if used: flexible bandage (Coban, 3M, St. Paul, USA)
REMOVAL OF TEST SUBSTANCE
- Washing (if done): dry and moistened tissue
- Time after start of exposure:
SCORING SYSTEM: - Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- overall irritation score
- Basis:
- animal #1
- Time point:
- 24 h
- Score:
- 0
- Remarks on result:
- other: no adverse effects to skin were observed
- Irritation parameter:
- overall irritation score
- Basis:
- animal #2
- Time point:
- 24 h
- Score:
- 0
- Remarks on result:
- other: no adverse effects to skin were observed
- Irritation parameter:
- overall irritation score
- Basis:
- animal #3
- Time point:
- 24 h
- Score:
- 0
- Remarks on result:
- other: no adverse effects to skin were observed
- Irritation parameter:
- overall irritation score
- Basis:
- animal #1
- Time point:
- 72 h
- Score:
- 0
- Remarks on result:
- other: no adverse effects to skin were observed
- Irritation parameter:
- overall irritation score
- Basis:
- animal #2
- Time point:
- 72 h
- Score:
- 0
- Remarks on result:
- other: no adverse effects to skin were observed
- Irritation parameter:
- overall irritation score
- Basis:
- animal #3
- Time point:
- 72 h
- Score:
- 0
- Remarks on result:
- other: no adverse effects to skin were observed
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The presented guideline study is reliable and adequate for the chemical assessement of 6-phenyl-1,3,5-triazine-2,4-diyldiamine. Based on the observations of the study the test substance is classified as non irritating to skin.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30.6.1988-3.7.1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was preformed according to a EU guideline study with GLP compliance.
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- Version / remarks:
- 84/449/EEC
- GLP compliance:
- yes
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Broekman Institute, Someren, NL
- Age at study initiation: approx. 18 weeks
- Weight at study initiation: animal #1 (4545-F): 3520 g; animal #2 (4561-F): 3503 g; animal #3 (4575-F): 3470 g
- Housing: individual housing in a plastic cage with perforated floor
- Diet (e.g. ad libitum): 100 g/day LKK-20, diameter 4 mm
- Water (e.g. ad libitum): free access to tap-water (via automatic nozzles)
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-20 °C
- Humidity (%): 65-80 %
- Air changes (per hr): no information available
- Photoperiod (hrs dark / hrs light): 12 h light, 12 h dark - Vehicle:
- unchanged (no vehicle)
- Remarks:
- test substance was ground to a fine powder
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- Three potions of 94±2 mg of the test substance were dispensed in glass containers with screw caps. Each potion was instilled in the conjunctival sac of the right eye of each animal using a spatula. The lids were then held gently together for two seconds and released. Immediately after treatment, the animals were transferred to their cages.
- Observation period (in vivo):
- 72 h; examinations after 60 min, 24, 48, and 72 hours
- Number of animals or in vitro replicates:
- three female rabbits
- Irritation parameter:
- maximum mean total score (MMTS)
- Basis:
- mean
- Time point:
- other: 60 mins
- Score:
- 4
- Max. score:
- 6
- Reversibility:
- fully reversible
- Irritation parameter:
- maximum mean total score (MMTS)
- Basis:
- mean
- Time point:
- 24 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- fully reversible
- Irritation parameter:
- maximum mean total score (MMTS)
- Basis:
- mean
- Time point:
- 48 h
- Score:
- 1.3
- Max. score:
- 2
- Reversibility:
- fully reversible
- Irritation parameter:
- maximum mean total score (MMTS)
- Basis:
- mean
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- fully reversible
- Interpretation of results:
- slightly irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: other: 67/548/EEC
- Conclusions:
- The presented guideline study is reliable and adequate for the chemical safety assessment of 6-phenyl-1,3,5-triazine-2,4-diyldiamine.
The fully reversibility of the chemosis within 24 hours indicates only mechanical irritation. The data are conclusive but not sufficient for classification according to Regulation 67/548/EEC.
Reference
No adverse effects on the cornea and the iris were observed in any of the rabbits during the entire observation period. Treatment of the eyes with fluorescein 24 hours after instillation of the test substance did not reveal any corneal epithelial damage. No signs of systemic intoxication were observed in any of the rabbits.
Approx. 60 minutes after exposure slight chemosis in animal #2 and #3 were observed which resolved within 24 hours.
Animal No. | 1 hour | 1 day | 2 day | 3 day | |
#1 | Cornea | 0 | 0 | 0 | 0 |
Iris | 0 | 0 | 0 | 0 | |
Conjunctivae | 2 | 2 | 2 | 0 | |
Subtotal | 2 | 2 | 2 | 0 | |
#2 | Cornea | 0 | 0 | 0 | 0 |
Iris | 0 | 0 | 0 | 0 | |
Conjunctivae | 6 | 2 | 0 | 0 | |
Subtotal | 6 | 2 | 0 | 0 | |
#3 | Cornea | 0 | 0 | 0 | 0 |
Iris | 0 | 0 | 0 | 0 | |
Conjuntivae | 4 | 2 | 2 | 0 | |
Subtotal | 4 | 2 | 2 | 0 | |
Total | 12 | 6 | 4 | 0 | |
Mean total | 4.0 | 2.0 | 1.3 | 0 |
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
The skin irritation test [Daamen, P.A.M. (1988a)] was identified as key study because it was well conducted and described in detail.
In this study the substance was studied for skin irritation in rabbits. The substance was applied to the skin with patches for 4 hours by three female rabbits. The animals were observed at 1h, 24h, 48h and 72 h after removal of the patches. During the observation period no adverse effects to skin were observed.
Due to the result of the test, the substance is classified as not irritating to skin.
The eye irritation study [Daamen, P.A.M. (1988b)] was identified as key study because it was well conducted and described in detail.
In this study the substance was studied for eye irritation in rabbits. The substance was dispensed, in the conjunctive sac of one eye of three female rabbits using a spatula. The animals were examined at 1h, 24h, 48h and 72 h after treatment.
No adverse effects on the cornea and the iris were observed in any of the rabbits during the entire observation period. No signs of systemic intoxication were observed in any of the rabbits. Approx. 60 minutes after exposure slight chemosis in two animals were observed which resolved within 24 hours.
In the line with the result the substance is described as slightly irritant but not sufficient for a classification according to Regulation (EC) No. 1272/2008.
Justification for selection of skin irritation / corrosion
endpoint:
The study was performed according to OECD guideline study with
Klimisch score 1.
Justification for selection of eye irritation endpoint:
The study was performed according to EU guideline study with
Klimisch score 1.
Effects on eye irritation: slightly irritating
Justification for classification or non-classification
The presented data for skin and the eye irritation are reliable and adequate guideline studies. The observations within these studies are conclusive but not sufficient for a classification according to Regulation (EC) No. 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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