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EC number: 240-299-7 | CAS number: 16143-80-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from publication
Data source
Reference
- Reference Type:
- publication
- Title:
- Evaluation of Subchronic (13 Week), Reproductive, and in Vitro Genetic Toxicity Potential of 2-Ethylhexyl-2-cyano-3,3-diphenyl Acrylate (Octocrylene)
- Author:
- Odio RM et al.
- Year:
- 1 994
- Bibliographic source:
- Fund Appl Toxicol 22: 355-368.1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- To evaluate the mutation potential of Octocrylene in mammalian cell by mammalian cell gene mutation assay.
- GLP compliance:
- not specified
- Type of assay:
- other: mammalian cell gene mutation assay
Test material
- Reference substance name:
- Octocrilene
- EC Number:
- 228-250-8
- EC Name:
- Octocrilene
- Cas Number:
- 6197-30-4
- Molecular formula:
- C24H27NO2
- IUPAC Name:
- 2-ethylhexyl 2-cyano-3,3-diphenylacrylate
- Details on test material:
- - Name of test material (as cited in study report): Octocrylene
- Molecular formula: C24H27NO2
- Molecular weight: 361.482
- Substance type: Organic
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Octocrylene
- Molecular formula: C24H27NO2
- Molecular weight: 361.482
- Substance type: Organic
Method
- Target gene:
- thymidine kinase locus in L5178Y mouse lymphoma
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Details on mammalian cell type (if applicable):
- - Type and identity of media: mouse lymphoma L5178Y TK+/_ cells supplied by Dr.Donald clive (Research Triangle park ,NC)
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- No data available
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver S-9
- Test concentrations with justification for top dose:
- 28 - 380 µg/ml ( without S-9)6.7 - 89 µg/ml (with S-9)
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- other: DMBA (with metabolic activation)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Exposure duration: 4 h
- Expression time (cells in growth medium): 24, 48 h
NUMBER OF CELLS EVALUATED: 200 cells/plate
DETERMINATION OF CYTOTOXICITY - Method: relative total growth - Rationale for test conditions:
- No data available.
- Evaluation criteria:
- Doubling of mutant frequency above control
- Statistics:
- Yes ,standard deviation was observed.
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- Additional information on results
RANGE-FINDING/SCREENING STUDIES: A preliminary study was conducted in mouse lymphoma L5178Y cells .The cells were exposed to eight concentration of test material ranging from 5-10000 µg /ml for 4 hour in the presence and absence of metabolic activator. From the
Preliminary study high concentration was selected 0.38mg/ml and 0.089 mg/ml for the cloning assay to be done in the presence and absence of metabolic activator. - Remarks on result:
- other: No increase in mutant frequency above solvent control was observed with the test substance in both the presence and absence of metabolic activation. The respective positive controls produced the expected significantly elevated mutant frequency.
Any other information on results incl. tables
Mutant frequency inMouse lymphoma L5178Y TK+/_cells exposed in vitro to Octocrylene (No metabolic activation)
|
Compound |
Concentration (µg/ml) |
Colonies/viable TFT plateb |
Colonies/viable Count/platec |
Mutant frequencyd
|
Relative total growthe |
1 |
Control(DMSO) |
1%v/v |
23±4 |
171±7 |
27 |
- |
2 |
Octocrylene |
0.38 |
20±1 |
83±11 |
48 |
6 |
|
|
0.28 |
21±3 |
104±3 |
40 |
9 |
0.21 |
26±3 |
118±3 |
44 |
12 |
||
0.16 |
29±9 |
128±7 |
45 |
13 |
||
0.12 |
25±0 |
130±8 |
38 |
14 |
||
0.089 |
24±1 |
132±13 |
36 |
18 |
||
0.067 |
26±3 |
144±12 |
36 |
20 |
||
0.050 |
27±4 |
148±11 |
36 |
23 |
||
0.038 |
21±3 |
136±8 |
31 |
27 |
||
0.028 |
22±2 |
167±11 |
26 |
50 |
||
3 |
EMS |
0.500 |
272±10 |
85±5 |
640* |
30 |
|
|
0.250 |
209±12 |
123±3 |
340* |
54 |
Mutant frequency in Mouse lymphoma L5178Y TK+/_ cells exposed in vitro to Octocrylene (No metabolic activation)
|
Compound |
Concentration (µg/ml |
Colonies/viable TFT plateb |
Colonies/viable Count/platec |
Mutant frequencyd
|
Relative total growthe |
1 |
Control(DMSO) |
1%v/v |
27±2 |
160±8 |
34 |
- |
2 |
Octocrylene |
0.089 |
23±2 |
137±6 |
34 |
9 |
|
0.067 |
22±3 |
134±6 |
33 |
28 |
|
|
0.050 |
19±3 |
150±7 |
25 |
58 |
|
|
0.038 |
21±3 |
163±7 |
26 |
94 |
|
|
0.028 |
23±1 |
154±7 |
30 |
96 |
|
|
0.021 |
21±2 |
150±5 |
28 |
97 |
|
|
0.016 |
26±3 |
143±3 |
36 |
91 |
|
|
0.012 |
24±4 |
166±8 |
29 |
99 |
|
|
0.0089 |
26±4 |
152±11 |
34 |
96 |
|
|
0.0067 |
27±2 |
169±15 |
32 |
105 |
|
|
EMS |
0.500 |
140±3 |
76±6 |
368* |
25 |
|
|
0.250 |
107±10 |
133±9 |
161* |
72 |
b; Cells plated in TFT containing medium; data are means ± Standard deviation of two or three plates
c; Cells plated in TFT free medium medium for viability determination ; data are means ± Standard deviation of two or three plates.
d; Mutant frequencies are reported as number of mutant per 106Surviving cell.
e; Relative total growth was determined as (%growth in suspension phase /% growth cloner phase) 100.Clonal phage growth determined from viable count plate.
*Significant different from vehicle control
b; Cells plated in TFT containing medium; data are means ± Standard deviation of two or three plates
c; Cells plated in TFT free medium medium for viability determination ; data are means ± Standard deviation of two or three plates.
D; Mutant frequencies are reported as number of mutant per 106Surviving cell.
E; Relative total growth was determined as (%growth in suspension phase /% growth cloner phase) 100.Clonal phage growth determined from viable count plate.
*Significant different from vehicle control
Applicant's summary and conclusion
- Conclusions:
- Octocrylene was observed for its mutagenic potential in mammalian cell. The test material was considered to be negative for mutagenic effect in the presence and absence of metabolic activator in Mouse lymphoma L5178Y TK+/_ cells.
- Executive summary:
In genetox study Octocrylene (6197-30-4) was assessed for its possible mutagenic potential. For this purpose mammalian cell gene mutation assay was performed inmouse lymphoma L5178Y TK+/_cells ,using a test substance concentration28 - 380 µg/ml ( without S-9)6.7 - 89 µg/ml (with S-9). Cytotoxicity was also observed by relative total growth method. No significant mutagenic effects were observed for Octocrylenein the presence and absence of metabolic activator in Mouse lymphoma L5178Y TK+/_cells. Therefore Octocrylene (6197-30-4) was considered to be non mutagenic with and without metabolic activator in Mouse lymphoma L5178Y TK+/_cells.
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