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EC number: 289-339-5 | CAS number: 87741-01-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- other: EU Risk Assessment
- Adequacy of study:
- other information
- Reliability:
- other:
Cross-reference
- Reason / purpose for cross-reference:
- reference to other study
Data source
Referenceopen allclose all
- Reference Type:
- other: EU Risk Assessment
- Title:
- European Union Risk Assessment Report 1,3-BUTADIENE, CAS No: 106-99-0, EINECS No:203-450-8
- Author:
- [ECB] European Chemicals Bureau
- Year:
- 2 002
- Reference Type:
- review article or handbook
- Title:
- Overview of reproductive and developmental toxicity studies of 1,3-butadiene in rodents.
- Author:
- Morrissey, R.E.
- Year:
- 1 990
- Bibliographic source:
- Environ Health Persp. 86; 79-84.
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Reference Type:
- publication
- Title:
- Ovarian toxicity and carcinogenicity in eight recent National Toxicology Program studies.
- Author:
- Maronpot, R.R.
- Year:
- 1 987
- Bibliographic source:
- Environ Health Persp. 73; 125-130.
- Reference Type:
- publication
- Title:
- Multiple organ carcinogenicity of 1,3-butadiene in B6C3F1 mice after 60 weeks of inhalation exposure.
- Author:
- Huff, et al.
- Year:
- 1 985
- Bibliographic source:
- Science. 227; 548-549.
- Reference Type:
- publication
- Title:
- Toxicology and carcinogenesis studies of 1,3-butadiene in B6C3F1 mice (inhalation studies).
- Author:
- NTP
- Year:
- 1 993
- Bibliographic source:
- NTP Technical Report Series no 434, NIH Publication 93-3165, Research Triangle Park, NC, USA
Materials and methods
- Principles of method if other than guideline:
- EU Risk Assessment on the major component of C4 Hydrocarbons: 1,3-Butadiene
- Type of method:
- in vivo
Test material
- Reference substance name:
- Buta-1,3-diene
- EC Number:
- 203-450-8
- EC Name:
- Buta-1,3-diene
- Cas Number:
- 106-99-0
- Molecular formula:
- C4H6
- IUPAC Name:
- buta-1,3-diene
- Details on test material:
- - Name of test material (as cited in study report): 1,3-butadiene
- CAS number: 106-99-0
- EINECS number: 203-450-8
Constituent 1
Test animals
- Species:
- mouse
Administration / exposure
- Route of administration:
- inhalation: gas
Results and discussion
Any other information on results incl. tables
A sperm-head morphology assay was conducted in mice (Hackett et al., 1988a). Details of the study are provided by Morissey et al. (1990).
Groups of 20 male mice were exposed to 0, 200, 1,000 or 5,000 ppm butadiene 6 hours/day for 5 days and sacrificed in the fifth week postexposure. Mice were examined for lesions of the reproductive tract and for gross tissue abnormalities. At least 500 sperm heads per mouse were examined for morphological abnormalities. Transient signs of toxicity, piloerection and dyspnea, were observed immediately following exposure to 5,000 ppm butadiene, but there were no mortalities and no effect on bodyweight in any groups compared with control.
There was a concentration- related increase in the percentage of abnormal sperm in exposed mice, with an increase of 21% in abnormal sperm at 200 ppm, 73% at 1,000 ppm and 129% at 5,000 ppm, relative to controls. The increases at 1,000 and 5,000 ppm were statistically significant.
Information on the effects of butadiene on reproductive organs is available from NTP and IISRP toxicity and carcinogenicity studies (Maronpot, 1987). In one NTP study, ovarian atrophy, hyperplasia and neoplasia in female mice and testicular atrophy in male mice were reported following exposure to 625 or 1,250 ppm butadiene for 60-61 weeks (Huff et al., 1985). A NOAEL for these effects was not identified from this study.
In the second NTP bioassay, mice were exposed to 0, 6.25, 20, 62.5, 200 or 625 ppm butadiene 6 hours/day, 5 days/week for up to 2 years (NTP, 1993). Interim sacrifices of 10 animals per sex per group were performed at 9 and 15 months, except at 625 ppm, when the reduction in survival was such that 2-8 animals were examined. In females, a statistically significantly increased incidence of ovarian atrophy was seen at 200 and 625 ppm at the 9-month sacrifice and at 62.5 ppm and above at the 15-month interim sacrifice. After 2 years, the incidence of ovarian atrophy showed a dose-related statistically significantly increase in all exposure groups, from 6.25 ppm upwards and it was reported that affected females had no evidence of oocytes, follicles or corpora lutea. After 2 years, the incidence of ovarian atrophy was 4/49 in controls compared with 19/49, 32/48, 42/50, 43/50 and 69/79 at 6.25, 20, 62.5, 200 and 625 ppm butadiene respectively. Uterine atrophy also occurred with increased incidence at 200 and 625 ppm, compared with controls, at 9 and 15 months and after 2 years of exposure. The incidence at 2 years was 1/50 in controls, compared with 8/50 and 41/78 at 200 and 625 ppm respectively. In males, there was an increased incidence of testicular atrophy at 625 ppm exposure groups at both the 9 and 15-month interim sacrifices and at 2 years. At 2 years the incidence was 53/72 compared with 1/50 in controls. In view of the reduction in survival in both sexes at 20 ppm and above and the severity of the neoplastic response in both sexes at 20 ppm and above and also in females at 6.25 ppm, it is possible that the gonadal effects seen in this study are a secondary consequence of severe generalised toxicity, rather than a direct effect of butadiene on the reproductive system. No significant ovarian effects were seen in females following either 15 days or 14 weeks of exposure to 625, 1,250, 2,500, 5,000 or 8,000 ppm butadiene (Maronpot, 1987).
Leydig cell tumours were observed in the IISRP bioassay in rats, at 1,000 and 8,000 ppm (Owen, 1981; Owen and Glaister, 1990; Owen et al., 1987).
Applicant's summary and conclusion
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