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EC number: 289-969-0 | CAS number: 90046-02-9 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Juniperus oxycedrus, Cupressaceae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 October 2019 – 29 November 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 (control), 1.2, 2.4, 5.1, 10.8, 47.6 and 100 mg/L.
- Sampling method: During Range finding study and main study, TOC was analyzed at 0 hour (Day 0) and at 72 hour (Day 3) from samples of all test nominal loading rates. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
The test item is a complex mixture (UVCB substance) which was found to be partially miscible (with no precipitation) in test medium (OECD Alga Medium) according to a lab internal method. Therefore, the Algae Test was carried out with aqueous extracts (WAF = Water Accommodated Fraction) at various loading rates of the test item in test medium.
In order to determine the optimum mixing duration to be used for WAF preparation, a water accommodated fraction (WAF) equilibration trail was performed prior to exposure: One individual WAF was prepared at each of three loading levels 1.0, 50.0 and 100 mg/L. At 2, 4, 6 and 24 hours after initiation of mixing, mixing was stopped at respective time points and the solutions were allowed to settle for one hour. A sample of WAF was removed from each loading level and mixing was resumed. The concentration of organic carbon that had solubilized into the WAF from the test item was measured using the TOC analyzer described above.
Maximum TOC values of 2.718, 25.57 and 51.98 mg/L was obtained at the nominal loading rate of 1.0, 50.0 and 100.0 mg/L after 6 hours of stirring followed by 1 hour of settling period, hence 6 hours of stirring duration, followed by 1 hour of settling period was selected for test media preparation.
Test media preparation: For every test item concentration, individual WAF was prepared by adding the appropriate amount of test item to the test medium. Each mixing vessel was filled to achieve minimal headspace given the constraints of the vessels and closed with foil-covered stoppers. The mixture was stirred for 6 h (based on equilibration trail) using a magnetic stirrer at minimum of 100 rpm. The mixtures were allowed to settle and equilibrate to test temperature (before removing the test solution). The aqueous portions of WAFs were collected using steri-pipette and transferred to test vessels.
- Controls: OECD Alga Medium only.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): The test substance was not fully soluble at the tested loading rate. However the method of preparing the WAFs was designed to mimimise exposure to undissolved test material. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green alga Pseudokirchneriella subcapitata
- Strain: ATCC 22662
- Source (laboratory, culture collection): American Type Culture Collection.
- Method of cultivation: Alga stock culture (maintained in OECD alga medium) is periodically subcultured at least once in a week and maintained with the illumination and temperature of 4440 to 8880 lux and 21 to 24°C, respectively. From healthy axenic culture of alga, pre-culture was prepared of desired cell density to provide the inoculum for test cultures.
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): Same conditions as test. The algal cell density of pre-cultures (acclimatation period) for range finding study and main study was respectively 41850 and 42150 cells/mL at start.
- Any deformed or abnormal cells observed: Not reported. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
- Test temperature:
- 22.2ºC - 22.6ºC
- pH:
- 7.95 - 8.18
- Nominal and measured concentrations:
- Nominal loading rates: 0 (control), 1.2, 2.4, 5.1, 10.8, 47.6 and 100 mg/L WAF
- Details on test conditions:
- TEST SYSTEM
- Test vessel: erlenmeyer flask
- Material, size, headspace, fill volume: glass, 250 mL capacity, 100 mL fill volume.
- Aeration: Not specified.
- Initial cells density: 5495 cells/mL
- Control end cells density: 936833 cells/mL (average)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): Not applicable
GROWTH MEDIUM
- Standard medium used: yes (OECD TG 201 medium)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the OECD medium was prepared as per the OECD guideline 201.
- Culture medium different from test medium: No
- Intervals of water quality measurement: The temperature and light intensity was monitored once in a day during pre-culture and exposure. The pH of the test nominal loading rates was measured at the beginning (0 h) and at the end (72 h) of the exposure.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: No
- Photoperiod: Constant illumination
- Light intensity and quality: 5326 to 5935 lux was maintained with a universal white type fluorescent lamp
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Determination of cell concentrations: counting chamber (observation times: 24, 48 and 72 hours): Cell density was obtained by calculating the average cell count (by summating the number of cells in all the four large corner squares and divide the sum by 4) and multiplied with chamber conversion factor (CCF) and dilution factor: Cell Density (cells/mL) = Average cell count × 10000 (CCF) × Dilution factor.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: geometric factor of 2.1
- Justification for using less concentrations than requested by guideline: Not applicable; 6 concentrations used for the main test.
- Range finding study
- Test concentrations: 0 (control), 0.01, 0.1, 1.0, 10.0, 50.0 and 100.0 mg/L.
- Results used to determine the conditions for the definitive study: At the tested concentrations of 0.01, 0.1, 1.0, 10.0, 50.0 and 100.0 mg/L, the inhibition growth rate was calculated to be 0.09, 0.59, 1.18, 8.24, 59.05 and 100% respectively. - Reference substance (positive control):
- yes
- Remarks:
- (3, 5-Dichlorophenol )
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 26.68 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (water-accommodated fraction loading rate)
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence interval = [20.56 – 34.61]
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 5.69 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (water-accommodated fraction loading rate)
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence interval = [4.46 – 7.26]
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 7.04 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (water-accommodated fraction loading rate)
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% confidence interval = [5.36 – 9.24]
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 2.15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (water-accommodated fraction loading rate)
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% confidence interval = [1.45 – 3.19]
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 1.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (water-accommodated fraction loading rate)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOELR
- Effect conc.:
- 2.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (water-accommodated fraction loading rate)
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): Not reported. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes, the 72 hours growth rate inhibition and percent inhibition in yield lie within the validity criteria acceptance range and establish the acceptability of test system response and test procedures followed.
- ErC50-72 h=1.12 mg/L and EyC50-72 h=0.33 mg/L. - Reported statistics and error estimates:
- The 72 hours EL10, EL20 and EL50 values were calculated from the responses (growth rate and yield) data against log nominal loading rates using the Probit analysis method (Finney, 1971). NOELR and LOELR were determined by SPSS statistical procedure (t-test) for multiple sample comparison using individual replicate values of the specific growth rate.
- Validity criteria fulfilled:
- yes
- Remarks:
- (increase of biomass in control during 72h>16 fold; coefficient of variation of the mean specific growth rate among replicates in control (t0-t72)<7%; the mean of the replicate coefficients of variation in section-by-section growth rate in control < 35%)
- Conclusions:
- In an Alga Growth Inhibition Test with Pseudokirchneriella subcapitata green algae, the growth rate inhibition effect concentrations were determined to be 72 h-ErL50 = 26.68 mg/L and 72 h-ErL10 = 5.69 mg/L.
- Executive summary:
An Algae Growth Inhibition Test was performed with Pseudokirchneriella subcapitata green algae on the test item over a period of 72 h in static conditions according to OECD Guideline 201, following GLP. The test item is a UVCB substance which was found to be partially miscible in OECD Alga Medium which was used as test medium. Therefore, the test was carried out with aqueous extracts (WAF = Water Accommodated Fraction) at various loading rates of the test item in the test medium. Based on a preliminary WAF equilibration trail, individual WAF solutions for every test item concentration were prepared in closed vessels with minimal headspace and stirred for 6 h. Then, the mixtures were allowed to settle and equilibrate to test temperature and aqueous portions of WAFs were transferred to the test vessels. In a preliminary range finding study the average growth inhibition rates were 0.09, 0.59, 1.18, 8.24, 59.05 and 100% at the tested nominal loading rates of 0.01, 0.1, 1.0, 10.0, 50.0 and 100.0 mg/L, respectively. Based on these results, the main test was conducted at nominal loading rates of 1.2, 2.4, 5.1, 10.8, 47.6 and 100 mg/L along with control group. The algal growth (cell density) was assessed at 24, 48 and 72 hours post-exposure. Exponentially growing alga cells (5495 cells/mL) were exposed to the selected nominal loading rates in 3 replicates for the treatment groups and 6 replicates for the control group. Negative control consisted of test medium alone and a solution of 3, 5-Dichlorophenol was used as reference substance. A growth rate inhibition of 0.2, 2.7, 13.9, 28.5, 59.7 and 100 % was observed at the nominal loading rates 1.2, 2.4, 5.1, 10.8, 47.6 and 100 mg/L respectively as compared with the control. Similarly, inhibition in yield of 1.1, 13.0, 51.3, 77.4, 95.9 and 100% was observed at the nominal loading rates 1.2, 2.4, 5.1, 10.8, 47.6 and 100 mg/L respectively as compared with the control. All validity criteria were fulfilled. TOC was analyzed at 0 hour (Day 0) and at 72 hour (Day 3) from samples of all test nominal loading rates. Based on these results, the growth rate inhibition effect concentrations were determined to be 72 h-ErL10 = 5.69 mg/L (95% CL: 4.46 – 7.26), 72 h-ErL20 = 9.67 mg/L (95% CL: 7.82 – 11.96) and 72 h-ErL50 = 26.68 mg/L (95% CL: 20.56 – 34.61), and the yield inhibition effect concentrations were 72h-EyL10 = 2.15 mg/L (95% CL: 1.45 – 3.19), 72h-EyL20 = 3.26 mg/L (95% CL: 2.36 – 4.42) and 72h-EyL50 = 7.04 mg/L (95% CL: 5.36 – 9.24). Finally, the 72 hours LOELR and NOELR were determined to be 2.4 and 1.2 mg/L respectively based on growth rate.
Reference
Table 1: Mean cell density after exposure during range finding study
Group |
Nominal Loading Rate |
R |
Mean Cell Density (104Cells/mL) |
||
Observation Periods |
|||||
24 h |
48 h |
72 h |
|||
G1 |
0.0 |
R1 |
5.50 |
26.68 |
88.55 |
R2 |
4.68 |
28.60 |
85.25 |
||
R3 |
5.23 |
23.65 |
86.90 |
||
G2 |
0.01 |
R1 |
5.23 |
22.55 |
86.08 |
R2 |
4.95 |
23.93 |
86.90 |
||
G3 |
0.1 |
R1 |
4.68 |
22.83 |
83.33 |
R2 |
4.95 |
23.93 |
85.25 |
||
G4 |
1.0 |
R1 |
4.40 |
23.10 |
82.50 |
R2 |
4.68 |
23.38 |
81.13 |
||
G5 |
10.0 |
R1 |
3.85 |
20.35 |
58.58 |
R2 |
3.30 |
20.08 |
55.55 |
||
G6 |
50.0 |
R1 |
1.38 |
3.58 |
4.40 |
R2 |
1.10 |
4.40 |
4.13 |
||
G7 |
100.0 |
R1 |
0.00 |
0.00 |
0.00 |
R2 |
0.00 |
0.00 |
0.00 |
Table 2. Percent inhibition in cell density, growth rate and yield during range finding study
Group |
Nominal Loading Rate (mg/L) |
% inhibition in Yield |
% inhibition in Cell Density |
% inhibition in Growth Rate |
G1 |
0.0 |
- |
- |
- |
G2 |
0.01 |
0.48 |
0.47 |
0.09 |
G3 |
0.1 |
3.02 |
3.01 |
0.59 |
G4 |
1.0 |
5.89 |
5.85 |
1.18 |
G5 |
10.0 |
34.54 |
34.34 |
8.24 |
G6 |
50.0 |
95.65 |
95.09 |
59.05 |
G7 |
100.0 |
100.00 |
100.00 |
100.00 |
Table 3. Mean cell density after exposure during main study
Group |
Nominal Loading Rate |
R |
Mean Cell Density (104Cells/mL) |
||
Observation Periods |
|||||
24 h |
48 h |
72 h |
|||
G1 |
0.0 |
R1 |
6.33 |
25.58 |
96.53 |
R2 |
6.05 |
26.40 |
92.95 |
||
R3 |
5.78 |
27.23 |
93.23 |
||
R4 |
6.33 |
22.55 |
94.05 |
||
R5 |
5.23 |
23.93 |
92.68 |
||
R6 |
6.05 |
26.40 |
92.68 |
||
G2 |
1.2 |
R1 |
5.50 |
26.40 |
93.78 |
R2 |
6.33 |
29.15 |
93.23 |
||
R3 |
5.78 |
25.58 |
91.03 |
||
G3 |
2.4 |
R1 |
4.68 |
22.55 |
83.33 |
R2 |
4.40 |
20.90 |
82.78 |
||
R3 |
5.23 |
21.45 |
78.65 |
||
G4 |
5.1 |
R1 |
3.03 |
14.58 |
48.13 |
R2 |
3.03 |
15.13 |
48.13 |
||
R3 |
2.48 |
14.85 |
41.53 |
||
G5 |
10.8 |
R1 |
2.48 |
9.63 |
21.45 |
R2 |
1.93 |
9.08 |
22.00 |
||
R3 |
2.48 |
9.90 |
21.45 |
||
G6 |
47.6 |
R1 |
1.10 |
2.75 |
4.13 |
R2 |
1.38 |
2.20 |
5.23 |
||
R3 |
1.10 |
1.93 |
3.85 |
||
G7 |
100.0 |
R1 |
0.00 |
0.00 |
0.00 |
R2 |
0.00 |
0.00 |
0.00 |
||
R3 |
0.00 |
0.00 |
0.00 |
Table 4. Average specific growth rate during main study
Group |
Nominal Loading Rates |
R |
Growth Rate |
Mean Growth |
|||
Observation Intervals (Days) |
|||||||
0 to 1 |
1 to 2 |
2 to 3 |
0 to 3 |
||||
G1 |
0 |
1 |
2.44 |
1.40 |
1.33 |
1.72 |
1.71 |
2 |
2.40 |
1.47 |
1.26 |
1.71 |
|||
3 |
2.35 |
1.55 |
1.23 |
1.71 |
|||
4 |
2.44 |
1.27 |
1.43 |
1.71 |
|||
5 |
2.25 |
1.52 |
1.35 |
1.71 |
|||
6 |
2.40 |
1.47 |
1.26 |
1.71 |
|||
Mean |
2.38 |
1.45 |
1.31 |
1.71 |
|||
±SD |
0.07 |
0.10 |
0.07 |
0.01 |
|||
CV |
3.02 |
6.98 |
5.72 |
0.30 |
|||
G2 |
1.2 |
1 |
2.30 |
1.57 |
1.27 |
1.71 |
1.71 |
2 |
2.44 |
1.53 |
1.16 |
1.71 |
|||
3 |
2.35 |
1.49 |
1.27 |
1.70 |
|||
Mean |
2.37 |
1.53 |
1.23 |
1.71 |
|||
±SD |
0.07 |
0.04 |
0.06 |
0.01 |
|||
CV |
3.00 |
2.63 |
4.96 |
0.31 |
|||
G3 |
2.4 |
1 |
2.14 |
1.57 |
1.31 |
1.67 |
*1.67 |
2 |
2.08 |
1.56 |
1.38 |
1.67 |
|||
3 |
2.25 |
1.41 |
1.30 |
1.65 |
|||
Mean |
2.16 |
1.51 |
1.33 |
1.67 |
|||
±SD |
0.09 |
0.09 |
0.04 |
0.01 |
|||
CV |
4.04 |
5.87 |
3.20 |
0.63 |
|||
G4 |
5.1 |
1 |
1.71 |
1.57 |
1.19 |
1.49 |
*1.47 |
2 |
1.71 |
1.61 |
1.16 |
1.49 |
|||
3 |
1.51 |
1.79 |
1.03 |
1.44 |
|||
Mean |
1.64 |
1.66 |
1.13 |
1.47 |
|||
±SD |
0.12 |
0.12 |
0.09 |
0.03 |
|||
CV |
7.07 |
7.08 |
7.74 |
1.93 |
|||
G5 |
10.8 |
1 |
1.51 |
1.36 |
0.80 |
1.22 |
*1.22 |
2 |
1.25 |
1.55 |
0.89 |
1.23 |
|||
3 |
1.51 |
1.39 |
0.77 |
1.22 |
|||
Mean |
1.42 |
1.43 |
0.82 |
1.22 |
|||
±SD |
0.15 |
0.10 |
0.06 |
0.00 |
|||
CV |
10.21 |
7.26 |
7.12 |
0.40 |
|||
G6 |
47.6
|
1 |
0.69 |
0.92 |
0.41 |
0.67 |
*0.69 |
2 |
0.92 |
0.47 |
0.87 |
0.75 |
|||
3 |
0.69 |
0.56 |
0.69 |
0.65 |
|||
Mean |
0.77 |
0.65 |
0.65 |
0.69 |
|||
±SD |
0.13 |
0.24 |
0.23 |
0.05 |
|||
CV |
16.77 |
36.40 |
35.47 |
7.73 |
|||
G7 |
100.0 |
1 |
0.0 |
0.0 |
0.0 |
0.0 |
*0.0 |
2 |
0.0 |
0.0 |
0.0 |
0.0 |
|||
3 |
0.0 |
0.0 |
0.0 |
0.0 |
|||
Mean |
0.0 |
0.0 |
0.0 |
0.0 |
|||
±SD |
0.0 |
0.0 |
0.0 |
0.0 |
|||
CV |
0.0 |
0.0 |
0.0 |
0.0 |
±SD: Standard deviation; CV: Coefficient of Variation; R: Replicate;*: Statistical significance of >0.05
Table 5. Yield of alga during main study
Group |
Nominal Loading Rate |
R |
Cell density (Cells/mL) |
Yield |
|
0 h |
72 h |
||||
G1 |
0 |
1 |
5495 |
965250 |
959756 |
2 |
5495 |
929500 |
924006 |
||
3 |
5495 |
932250 |
926756 |
||
4 |
5495 |
940500 |
935006 |
||
5 |
5495 |
926750 |
921256 |
||
6 |
5495 |
926750 |
921256 |
||
|
Mean |
936833 |
931339 |
||
|
±SD |
14826 |
14826 |
||
G2 |
1.2 |
1 |
5495 |
937750 |
932256 |
2 |
5495 |
932250 |
926756 |
||
3 |
5495 |
910250 |
904756 |
||
|
Mean |
926750 |
921256 |
||
|
±SD |
14552 |
14552 |
||
G3 |
2.4 |
1 |
5495 |
833250 |
827756 |
2 |
5495 |
827750 |
822256 |
||
3 |
5495 |
786500 |
781006 |
||
|
Mean |
815833 |
810339 |
||
|
±SD |
25552 |
25552 |
||
G4 |
5.1 |
1 |
5495 |
481250 |
475756 |
2 |
5495 |
481250 |
475756 |
||
3 |
5495 |
415250 |
409756 |
||
|
Mean |
459250 |
453756 |
||
|
±SD |
38105 |
38105 |
||
G5 |
10.8 |
1 |
5495 |
214500 |
209006 |
2 |
5495 |
220000 |
214506 |
||
3 |
5495 |
214500 |
209006 |
||
|
Mean |
216333 |
210839 |
||
|
±SD |
3175 |
3175 |
||
G6 |
47.6 |
1 |
5495 |
41250 |
35756 |
2 |
5495 |
52250 |
46756 |
||
3 |
5495 |
38500 |
33006 |
||
|
Mean |
44000 |
38506 |
||
|
±SD |
7276 |
7276 |
||
G7 |
100.0 |
1 |
5495 |
0 |
-5495 |
2 |
5495 |
0 |
-5495 |
||
3 |
5495 |
0 |
-5495 |
||
|
Mean |
0 |
0 |
||
|
±SD |
0 |
0 |
Table 6. Percent inhibition in cell density, growth rate and yield during main study
Group |
Test Conc. (mg/L) |
% inhibition in Yield |
% inhibition in Cell Density |
% inhibition in Growth Rate |
G1 |
0 |
- |
- |
- |
G2 |
1.2 |
1.1 |
1.1 |
0.2 |
G3 |
2.4 |
13.0 |
12.9 |
2.7 |
G4 |
5.1 |
51.3 |
51.0 |
13.9 |
G5 |
10.8 |
77.4 |
76.9 |
28.5 |
G6 |
47.6 |
95.9 |
95.3 |
59.7 |
G7 |
100.0 |
100.0 |
100.0 |
100.0 |
Table 7. Acceptance criteria for control during main study
0 – 72 Hours Mean Coefficient of Variation of Section-By-Section Average Specific Growth Rate |
||
Daily average growth rate |
±SD |
% CV out of the daily growth rates |
1.72 |
0.62 |
36.27 |
1.71 |
0.61 |
35.43 |
1.71 |
0.58 |
33.76 |
1.71 |
0.64 |
37.12 |
1.71 |
0.48 |
27.94 |
1.71 |
0.61 |
35.51 |
Mean % of CV 0 – 72 hours |
34.34 |
Biomass increase during the whole test period |
|||
Initial biomass (cells/mL) |
Cell density on day 3 (cells/mL) |
Biomass increase Factor |
|
5495 |
965250 |
175.68 |
|
5495 |
929500 |
169.17 |
|
5495 |
932250 |
169.67 |
|
5495 |
940500 |
171.17 |
|
5495 |
926750 |
168.67 |
|
5495 |
926750 |
168.67 |
|
Mean |
936833.33 |
170.50 |
Table 8. TOC analysis during exposure
Dose Range Finding Study |
|||
Group |
Nominal loading Rate (mg/L) |
TOC Analysis in Test Media (mg/L) |
|
Day 0 |
Day 3 |
||
G1 |
0.0 |
- |
- |
G2 |
0.01 |
0.134 |
0.119 |
G3 |
0.1 |
0.782 |
0.647 |
G4 |
1.0 |
1.024 |
0.914 |
G5 |
10.0 |
8.691 |
9.695 |
G6 |
50.0 |
16.840 |
16.940 |
G7 |
100.0 |
31.260 |
31.300 |
Main Study |
|||
Group |
Nominal loading Rate (mg/L) |
TOC Analysis in Test Media (mg/L) |
|
Day 0 |
Day 3 |
||
G1 |
0.0 |
- |
- |
G2 |
1.2 |
1.084 |
0.935 |
G3 |
2.4 |
2.142 |
1.982 |
G4 |
5.1 |
3.118 |
3.176 |
G5 |
10.8 |
4.825 |
4.404 |
G6 |
47.6 |
28.960 |
21.410 |
G7 |
100.0 |
68.630 |
48.130 |
Table 9. Temperature and light intensity during pre-culture and exposure main study
Day |
Temperature of the surrogate (°C) |
Light intensity (Lux) |
Revolutions per minute (rpm) |
|||
1 |
2 |
3 |
4 |
|||
During Acclimatisation (pre-culture) & Exposure |
||||||
0 |
22.5 |
5841 |
5935 |
5629 |
5711 |
100 |
1 |
22.4 |
5489 |
5382 |
5921 |
5827 |
100 |
2 |
22.6 |
5654 |
5829 |
5713 |
5889 |
100 |
3 |
22.5 |
5826 |
5758 |
5656 |
5893 |
100 |
4 |
22.2 |
5744 |
5627 |
5758 |
5647 |
100 |
5 |
22.2 |
5465 |
5497 |
5692 |
5326 |
100 |
6 |
22.4 |
5682 |
5589 |
5642 |
5486 |
100 |
Mean |
22.4 |
5672 |
5660 |
5716 |
5683 |
- |
±SD |
0.2 |
149.6 |
193.1 |
100.8 |
214.3 |
- |
Min |
22.2 |
5326 |
||||
Max |
22.6 |
5935 |
Table 10. pH of the test medium during main study
Group |
Nominal Loading Rate (mg/L) |
pH |
|||||||||||
0 hours |
72 hours |
||||||||||||
R1 |
R2 |
R3 |
R4 |
R5 |
R6 |
R1 |
R2 |
R3 |
R4 |
R5 |
R6 |
||
G1 |
0 |
8.12 |
8.15 |
8.10 |
8.11 |
8.09 |
8.11 |
8.09 |
8.05 |
8.05 |
8.07 |
8.03 |
8.07 |
G2 |
1.5 |
8.10 |
8.17 |
8.18 |
- |
- |
- |
8.05 |
8.06 |
8.10 |
- |
- |
- |
G3 |
2.6 |
8.07 |
8.09 |
8.11 |
- |
- |
- |
8.03 |
8.04 |
8.05 |
- |
- |
- |
G4 |
4.8 |
8.08 |
8.09 |
8.09 |
- |
- |
- |
8.00 |
8.05 |
8.01 |
- |
- |
- |
G5 |
8.6 |
8.10 |
8.05 |
8.07 |
- |
- |
- |
8.11 |
8.07 |
8.05 |
- |
- |
- |
G6 |
15.4 |
8.07 |
8.10 |
8.11 |
- |
- |
- |
8.00 |
8.01 |
8.01 |
- |
- |
- |
G7 |
27.8 |
8.12 |
8.09 |
8.09 |
- |
- |
- |
7.98 |
7.95 |
8.00 |
- |
- |
- |
G8 |
100.0 |
8.09 |
8.06 |
8.1 |
- |
- |
- |
8.09 |
8.05 |
8.05 |
8.07 |
8.03 |
8.07 |
|
Min |
8.05 |
7.95 |
||||||||||
|
Max |
8.18 |
8.11 |
Description of key information
Key study. Test method according to OECD 201, GLP study. The growth rate inhibition effect concentrations in Pseudokirchneriella subcapitata green algae were determined to be 72 h-ErL50 = 26.68 mg/L (95% CL: 20.56 – 34.61) and 72 h-ErL10 = 5.69 mg/L (95% CL: 4.46 – 7.26).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 26.68 mg/L
- EC10 or NOEC for freshwater algae:
- 5.69 mg/L
Additional information
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