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EC number: 221-882-5 | CAS number: 3268-49-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- significant methodological deficiencies
- Remarks:
- Duration of exposure only 24 h.
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was directly added the te test medium. The solution was homogenised with an ultra-turrax. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- .TEST ORGANISM
- Common name: zebrafish
- Source: Centre de Recherche de la Station de Lagunage de Meze, Meze, France
- Length at study initiation (length definition, mean, range and SD): 3.5 ± 0.3 cm
- Weight at study initiation (mean and range, SD): 0.44 ± 0.21 g
ACCLIMATION
- Acclimation period: 14 d
- Acclimation conditions (same as test or not): dilution water at 23 °C ± 1°C
- Type and amount of food during acclimation: not given
- Feeding frequency during acclimation: daily except 24 h before the test
- Health during acclimation (any mortality observed): No desease was observed. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Test temperature:
- 21.9 - 22.8 °C
- pH:
- 7.44 - 7.84
- Dissolved oxygen:
- 93 - 96 % at 0 h
82 - 86 at 24 h - Nominal and measured concentrations:
- Nominal test substance concentrations: 0, 5.5, 9.1, 12.5, 18.1, 27.7, 40.3, 57.7 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquaria 28x28x28cm
- Fill volume: 10 L
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionised water, reconstitued with
CaCl2 x 2H2O = 0.294 g/L
MgSO4 x 7H2O = 0.12325 g/L
NaHCO3 = 0.06475 g/L
KCl =0.00575 g/L
- Intervals of water quality measurement: pH and qxygen at 0 and 24 h
OTHER TEST CONDITIONS
- Light intensity: 1000 to 2000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
- Mortality after 24 h
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1.1, 3.7, 10.1, 42.2, 106, 372, 1076 mg/L
- Results used to determine the conditions for the definitive study: LC50 (24 h): 21 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- LC0
- Effect conc.:
- 9.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 14 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC100
- Effect conc.:
- 18.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: starting at 4 h and a concentration of 40.3 mg/L slower swimming was observed. At 4h and a concentration of 57.7 mg/L swimming at the bottom of the test vessel and immobilised test fish were observed.
- Observations on body length and weight: lenght 3.5 ± 0.3 cm, weight: 0.44 ± 0.21 g - Results with reference substance (positive control):
- LC50 (24 h): 210 mg/L
- Reported statistics and error estimates:
- log-probit analysis
- Sublethal observations / clinical signs:
-
Table with the cumulative mortality:
concentration (mg/L)
Mortality (number of fish)
Mortality (%)
0
0
0
5.5
0
0
9.1
0
0
12.5
1
10
18.1
10
100
27.7
10
100
40.3
10
100
57.7
10
100
- Validity criteria fulfilled:
- no
- Conclusions:
- In a test conducted with Danio rerio the determined LC50 after 24 h was 14 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: At the beginning of exposure, before water change
- Vehicle:
- not specified
- Test organisms (species):
- Oryzias latipes
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 24 ± 1°C
- Nominal and measured concentrations:
- Nominal test substance concentrations: 3.95, 5.93, 8.89, 13.3 and 20.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Fill volume: 25 L
- Renewal rate of test solution (frequency): every 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark
- Light intensity: Indoor light
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5 - Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 6.95 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence limit: 5.95 to 8.33 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 3.19 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 12.3 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Referenceopen allclose all
Description of key information
LC50 (96 h): 6.95 mg/L (meas., OECD 203)
Key value for chemical safety assessment
Additional information
Two studies on the acute toxicity of the substance to freshwater fish are available. One study was conducted according to OECD 203 with Oryzas latipes in a semi-static test system. The nominal test substance concentrations of 3.95, 5.93, 8.89, 13.3 and 20.0 mg/L were re-newed every 24 hours. An analytical dose verification by HPLC analysis was performed. The determined LC50 (96 h) was 6.95 mg/L (measured). A second available test was performed according to ISO guideline 7346-1 (GLP). Zebrafish (Danio rerio) were exposed to nominal test substance concentrations of 0, 5.5, 9.1, 12.5, 18.1, 27.7, 40.3, 57.7 mg/L for 24 h. No analytical verification of the test item concentrations was performed. The mortality of fish was measured after 24 h resulting in a LC50 of 14 mg/L based on nominal concentrations.
However, since the test duration was only 24 h the study is not sufficient and this result is not used for the assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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