Registration Dossier
Registration Dossier
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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 203-095-9 | CAS number: 103-28-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- Toxicity to micro organism test was performed on Arthrobacter sp.,Corynebacterium minutissimum (CM) ,Staphylococ cus aureus (IAM-1011, (SA)) ,Staphylococcus epidermidis var. (SE),Escherichia coli (ATCC 11775, (EC)) for exposure period of 24 hrs. to determine the Minimum Inhibitory Concentration(MIC)
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Details on sampling:
- Sampling method: Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.
- Vehicle:
- yes
- Details on test solutions:
- - Chemical name of vehicle (organic solvent, emulsifier or dispersant):dimethyl sulfoxide (DMSO)
- Test organisms (species):
- other: Arthrobacter sp.,Corynebacterium minutissimum (CM) ,Staphylococ cus aureus (IAM-1011, (SA)) ,Staphylococcus epidermidis var. (SE),Escherichia coli (ATCC 11775, (EC))
- Test type:
- not specified
- Water media type:
- not specified
- Total exposure duration:
- 24 h
- Hardness:
- No data
- Test temperature:
- No data
- pH:
- No data
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- No data
- Details on test conditions:
- Test vessel: Agar plates
No. of organisms per vessel: microbial concentration of 106CFU/ml - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 24 h
- Dose descriptor:
- other: Minimum Inhibitory Concentration(MIC)
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- not specified
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The Minimum Inhibitory Concentration of test substance Benzyl isobutyrate for Arthrobacter sp.,Corynebacterium minutissimum (CM) ,Staphylococ cus aureus (IAM-1011, (SA)) ,Staphylococcus epidermidis var. (SE),Escherichia coli (ATCC 11775, (EC)) was determine to be > 2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure.
- Executive summary:
Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.
The Minimum Inhibitory Concentration of Arthrobacter sp.,Corynebacterium minutissimum (CM) ,Staphylococ cus aureus (IAM-1011, (SA)) ,Staphylococcus epidermidis var. (SE), Escherichia coli (ATCC 11775, (EC)) was determine to be > 2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to test chemical.
Reference
Description of key information
Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C. The Minimum Inhibitory Concentration of Arthrobacter sp.,Corynebacterium minutissimum (CM) ,Staphylococ cus aureus (IAM-1011, (SA)) ,Staphylococcus epidermidis var. (SE), Escherichia coli (ATCC 11775, (EC)) was determine to be > 2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to test chemical.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 2 000 mg/L
Additional information
Various studies available for the test chemical and similar read across chemicals were reviewed to determine the toxic nature of test chemical on the growth and other activity of microorganisms. The studies are as mentioned below:
Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C. The Minimum Inhibitory Concentration of Arthrobacter sp.,Corynebacterium minutissimum (CM) ,Staphylococ cus aureus (IAM-1011, (SA)) ,Staphylococcus epidermidis var. (SE), Escherichia coli (ATCC 11775, (EC)) was determine to be > 2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to test chemical.
The Minimum Inhibition (MIC) effect of test chemical was observed on Corynebacterium minutissimum (CM), Arthrobacter sp. isolated from Lipo-66, Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) for exposure period of 24 hrs. Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C. The Minimum Inhibitory Concentration of test chemical on Corynebacterium minutissimum (CM), Arthrobacter sp. isolated from Lipo-66, Staphylococcus aureus (IAM-1011, (SA)), Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) microorganisms species was determine to be >2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure with test chemical.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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