Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-095-9 | CAS number: 103-28-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from Peer-reviewed journal
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Acute oral toxicity of test chemical in rats
- Author:
- D. McGinty et. al.
- Year:
- 2 012
- Bibliographic source:
- Food and Chemical Toxicology,2012
- Reference Type:
- publication
- Title:
- Acute oral toxicity of test chemical in rats
- Author:
- D. Belsito et. al.
- Year:
- 2 012
- Bibliographic source:
- Food and Chemical Toxicology,2012
- Reference Type:
- other: authoritative database
- Title:
- Acute oral toxicity study of benzyl isobutyrate (103-28-6) in rat
- Author:
- U.S. National Library of Medicine
- Year:
- 2 018
- Bibliographic source:
- Chemidplus Database,U.S. National Library of Medicine,2018
- Reference Type:
- review article or handbook
- Title:
- Dictionary of Food Compounds with CD-ROM, Second Edition
- Author:
- Shmuel Yannai
- Year:
- 2 012
- Bibliographic source:
- Dictionary of Food Compounds with CD-ROM, Second Edition, CRC Press, page number 1334,23-Oct-2012
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Principles of method if other than guideline:
- Acute oral toxicity of test chemical was determined in rats.
- GLP compliance:
- not specified
- Test type:
- other: No data
- Limit test:
- no
Test material
- Reference substance name:
- Benzyl isobutyrate
- EC Number:
- 203-095-9
- EC Name:
- Benzyl isobutyrate
- Cas Number:
- 103-28-6
- Molecular formula:
- C11H14O2
- IUPAC Name:
- benzyl 2-methylpropanoate
- Test material form:
- other: Liquid
- Details on test material:
- - Name of test material (as cited in study report): Benzyl isobutyrate
- Molecular formula (if other than submission substance): C11H14O2
- Molecular weight (if other than submission substance): 178.23 g/mole
- Substance type: Organic
- Physical state: Liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- not specified
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- No data available
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- not specified
- Details on oral exposure:
- No data available
- Doses:
- 2510, 3160, 3980 and 5000 mg/kg
- No. of animals per sex per dose:
- Total:40
2510 mg/kg bw: 5 male, 5 female
3160 mg/kg bw: 5 male, 5 female
3980 mg/kg bw: 5 male, 5 female
5000 mg/kg bw: 5 male, 5 female - Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:No data
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs and gross pathology were observed. - Statistics:
- No data available
Results and discussion
- Preliminary study:
- In preliminary studies, dose levels of 316, 1260 and 5000 mg/kg resulted in mortality in 0/2, 0/2 and 2/2 rats from low to high dose.
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 850 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 2 252 - <= 3 608
- Remarks on result:
- other: No effect on survival, clinical sign and gross pathology
- Mortality:
- When treated with 5000 mg/kg bw, all animals were died, at 3980 mg/kg bw, 9 animals were died, at 3160 mg/kg bw, 6 animals were died and at 2510 mg/kg 4 animals were died.
- Clinical signs:
- other: sedation and tremors were observed in treated rats.
- Gross pathology:
- No gross pathological alterations were observed in treated male and female rats at necropsy.
- Other findings:
- No data available
Any other information on results incl. tables
Dose (mg/kg) |
Mortality / number of animals treated |
2510 |
4/10 |
3160 |
6/10 |
3980 |
9/10 |
5000 |
10/10 |
Summary of acute toxicity studies
Route |
Species |
NO. animals/dose group |
LD50 (g/kg) |
Oral |
Rat |
10 |
2.85 |
Applicant's summary and conclusion
- Interpretation of results:
- other: not classified
- Conclusions:
- Therefore, LD50 was considered to be 2850 mg/kg bw (2252-3608 mg/kg) when male and female rats were treated with test chemical orally by gavage.
- Executive summary:
Ina acute oral toxicity study, male and female rats were treated with test chemical in the concentration of 2510, 3160, 3980 or 5000 mg/kg orally by gavage and observed for 14 days. In preliminary studies, dose levels of 316, 1260 and 5000 mg/kg resulted in mortality in 0/2, 0/2 and 2/2 rats from low to high dose. In main study, all animals were died at 5000 mg/kg bw, 9 animals died at 3980 mg/kg bw, 6 animals died at 3160 mg/kg bw and 4 animals died at 2510 mg/kg. Sedation and tremors were observed in treated rats. No gross pathological alterations were observed in treated male and female rats at necropsy. Therefore, LD50 was considered to be 2850 mg/kg bw (2252-3608 mg/kg) when male and female rats were treated with test chemical orally by gavage.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.