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EC number: 939-690-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP - Guideline study, tested with the source substance Pentaerythritol tetra(2-ethylhexanoate). According to the ECHA guidance document "Practical guide 6: How to report read-across and categories (March 2010)", the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,2-bis[[(2-ethyl-1-oxohexyl)oxy]methyl]propane-1,3-diyl bis(2-ethylhexanoate)
- EC Number:
- 230-743-8
- EC Name:
- 2,2-bis[[(2-ethyl-1-oxohexyl)oxy]methyl]propane-1,3-diyl bis(2-ethylhexanoate)
- Cas Number:
- 7299-99-2
- Molecular formula:
- C37H68O8
- IUPAC Name:
- 3-[(2-ethylhexanoyl)oxy]-2,2-bis{[(2-ethylhexanoyl)oxy]methyl}propyl 2-ethylhexanoate
- Details on test material:
- - Name of test material (as cited in study report): Pentaerythritol tetra(2-ethylhexanoate)
- Analytical purity: 98.4 %
- Physical state: colourless liquid
- Analytical purity: 98.4% (GC)
- Lot/batch No.: TOL-887
- Stability under test conditions: proven by re-analysis
- Storage condition of test material: airtight at room temperature in the dark
Constituent 1
Method
- Target gene:
- his operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of rats treated with phenobarbital and 5,6-benzoflavone
- Test concentrations with justification for top dose:
- Dose-finding study:
-S9 mix: 0, 8.19, 20.5, 51.2, 128, 320, 800, 2000, 5000 μg/plate (all strains)
+S9 mix: 0, 8.19, 20.5, 51.2, 128, 320, 800, 2000, 5000 μg/plate (all strains)
Main study:
-S9 mix: 0, 78.1, 156, 313, 625, 1250, 2500 μg/plate (all strains)
+S9 mix: 0, 78.1, 156, 313, 625, 1250, 2500 μg/plate (all strains)
Re-study:
-S9 mix; 0, 78.1, 156, 313, 625, 1250, 2500 μg/plate (TA1535) - Vehicle / solvent:
- acetone
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: -S9 mix; 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2, for TA100, TA98, WP2 uvrA), sodium azide (NaN3, for TA1535) and 9-aminoacridine hydrochloride (9-AA, for TA1537) +S9 mix; 2-Aminoanthracene (2-AA, for all strains)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20 min, 37°C
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
- Method: reduction of background lawn
OTHER:
A re-test with TA1535 without S9 mix was performed to show reproducibility. - Evaluation criteria:
- The test substance was considered positive if the number of revertant colonies increased more than double compared to the number of revertant of the vehicle control as well as a dose-dependeny was found.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- precipitation was observed at concentrations of ≥ 1250 µg/plate at the end of the exposure period
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- precipitation was observed at concentrations of ≥ 1250 µg/plate at the end of the exposure period
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- No increase in revertant colonies was observed in the test with either the non-activation method (-S9 mix) or the activation method (+S9 mix).
TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: deposits of oily droplets were oberseved at concentrations including and higher than 1250 µg/plate
RANGE-FINDING/SCREENING STUDIES:
Since contamination was found in strain TA1535 without S9 mix, a retest with this strain was performed. However, no increase in number of revertants was found in any strain. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Pretest:
With or without S9-Mix |
Test substance concentration |
Mean number of revertant colonies per plate |
|||||
(μg/plate) |
(average of 3 plates ± Standard deviation) |
||||||
|
Base-pair substitution type |
Frameshift type |
|||||
|
TA 100 |
TA1535 |
WP2uvrA |
TA98 |
TA1537 |
||
– |
0 |
120 ± 10 |
- |
25 ± 3 |
22 ± 1 |
7 ± 3 |
|
– |
8.19 |
18 ± 6 |
- |
24 ± 3 |
22 ± 2 |
5 ± 1 |
|
– |
20.5 |
126 ± 7 |
- |
24 ± 3 |
23 ± 4 |
7 ± 3 |
|
– |
51.2 |
110 ± 4 |
- |
26 ± 4 |
30 ± 2 |
7 ± 1 |
|
– |
128 |
117 ± 8 |
- |
30 ± 2 |
26 ± 3 |
7 ± 2 |
|
– |
320 |
117 ± 9 |
- |
23 ± 2 |
23 ± 3 |
5 ± 2 |
|
– |
800 |
118 ± 9 |
- |
22 ± 3 |
25 ± 3 |
7 ± 3 |
|
– |
2000 + |
113 ± 4 |
- |
28 ± 3 |
28 ± 4 |
8 ± 3 |
|
– |
5000 + |
118 ± 3 |
|
24 ± 3 |
25 ± 3 |
8 ± 2 |
|
Positive controls, –S9 |
Name |
AF-2 |
- |
AF-2 |
AF-2 |
9-AA |
|
Concentrations (μg/plate) |
0.01 |
- |
0.01 |
0.1 |
80 |
||
Mean No. of colonies/plate (average of 3 ± SD) |
590 ± 25 |
- |
151 ± 9 |
706 ± 25 |
197 ± 25 |
||
+ |
0 |
121 ± 8 |
12 ± 2 |
24 ± 2 |
28 ± 5 |
14 ± 1 |
|
+ |
8.19 |
132 ± 6 |
10 ± 2 |
22 ± 2 |
34 ± 3 |
12 ± 2 |
|
+ |
20.5 |
124 ± 7 |
10 ± 2 |
28 ± 3 |
33 v 3 |
14 ± 3 |
|
+ |
51.2 |
127 ± 2 |
12 ± 2 |
30 ± 4 |
35 ± 1 |
12 ± 3 |
|
+ |
128 |
121 ± 4 |
12 ± 2 |
25 ± 3 |
30 ± 3 |
11 ± 1 |
|
+ |
320 |
126 ± 11 |
12 ± 3 |
25 ± 4 |
28 ± 3 |
12 ± 2 |
|
+ |
800 |
139 ± 11 |
11 ± 1 |
25 ± 2 |
28 ± 4 |
12 ± 2 |
|
+ |
2000 + |
132 ± 4 |
11 ± 1 |
28 ± 5 |
31 ± 3 |
13 ± 2 |
|
+ |
5000 + |
134 ± 7 |
9 ± 3 |
31 ± 2 |
30 ± 3 |
14 ± 2 |
|
Positive controls, +S9 |
Name |
2-AA |
2-AA |
2-AA |
2-AA |
2-AA |
|
Concentrations (μg/plate) |
1 |
2 |
10 |
0.5 |
2 |
||
Mean No. of colonies/plate (average of 3 ± SD) |
1044 ± 114 |
304 ± 24 |
682 ± 17 |
359 ± 13 |
135 ± 12 |
+: precipitation
AF-2:2 -(2 -furyl)-3 -(5 -nitro-2 -furyl) acrylamide
NaN3: Sodium azide
9 -AA:9 - aminoacridine hydrochloride
2 -AA.2 - aminoanthracene
Main test:
With or without S9-Mix |
Test substance concentration |
Mean number of revertant colonies per plate |
|||||
(μg/plate) |
(average of 3 plates ± Standard deviation) |
||||||
|
Base-pair substitution type |
Frameshift type |
|||||
|
TA 100 |
TA1535 |
WP2uvrA |
TA98 |
TA1537 |
||
– |
0 |
119 ± 5 |
10 ± 1 |
24 ± 3 |
20 v 1 |
7 ± 1 |
|
– |
78.1 |
126 ± 12 |
9 ± 3 |
27 ± 3 |
19 ± 3 |
9 ± 4 |
|
– |
156 |
120 ± 11 |
14 ± 1 |
24 ± 2 |
22 ± 2 |
8 ± 1 |
|
– |
313 |
123 ± 7 |
13 ± 1 |
23 ± 3 |
17 ± 3 |
6 ± 1 |
|
– |
625 |
122 ± 16 |
11 ± 3 |
23 ± 2 |
17 ± 2 |
7 ± 1 |
|
– |
1250 + |
112 ± 3 |
13 ± 3 |
26 ± 3 |
24 ± 6 |
7 ± 1 |
|
– |
2500 + |
128 ± 9 |
11 ± 2 |
27 ± 4 |
22 ± 3 |
9 v 3 |
|
Positive controls, –S9 |
Name |
AF-2 |
NaN3 |
AF-2 |
AF-2 |
9-AA |
|
Concentrations (μg/plate) |
0.01 |
0.5 |
0.01 |
0.1 |
80 |
||
Mean No. of colonies/plate (average of 3 ± SD) |
741 ± 57 |
436 ± 25 |
152 ± 12 |
689 ± 53 |
205 ± 6 |
||
+ |
0 |
131 ± 5 |
11 ± 2 |
29 ± 8 |
30 ± 5 |
12 ± 2 |
|
+ |
78.1 |
132 ± 13 |
12 ± 1 |
27 ± 9 |
30 ± 4 |
13 ± 4 |
|
+ |
156 |
145 ± 5 |
12 ± 1 |
24 ± 4 |
34 ± 5 |
10 ± 3 |
|
+ |
313 |
131 ± 3 |
13 ± 2 |
26 ± 6 |
29 ± 2 |
16 ± 5 |
|
+ |
625 |
148 ± 6 |
12 ± 2 |
24 ± 3 |
26 ± 1 |
13 ± 3 |
|
+ |
1250 + |
135 ± 145 |
10 ± 1 |
27 ± 4 |
28 ± 3 |
11 ± 3 |
|
+ |
2500 + |
152 ± 3 |
10 ± 2 |
31 ± 4 |
28 ± 3 |
14 ± 4 |
|
Positive controls, +S9 |
Name |
2-AA |
2-AA |
2-AA |
2-AA |
|
|
Concentrations (μg/plate) |
1 |
2 |
10 |
0.5 |
2 |
||
Mean No. of colonies/plate (average of 3 ± SD) |
1047 ± 53 |
362 ± 6 |
720 ± 49 |
343 ± 45 |
129 ± 13 |
+: precipitation
AF-2: 2 -(2 -furyl)-3 -(5 -nitro-2 -furyl) acrylamide
NaN3: Sodium azide
9 -AA: 9 - aminoacridine hydrochloride
2 -AA. 2 - aminoanthracene
Re-test:
With or without S9-Mix |
Test substance concentration |
Mean number of revertant colonies per plate |
|
(μg/plate) |
(average of 3 plates ± Standard deviation) |
||
|
Base-pair substitution type |
||
|
TA1535 |
||
– |
0 |
9 ± 2 |
|
– |
78.1 |
8 ± 1 |
|
– |
156 |
13 ± 2 |
|
– |
313 |
13 ± 1 |
|
– |
625 |
11 ± 3 |
|
– |
1250 |
10 ± 1 |
|
– |
2500 |
10 ± 3 |
|
Positive controls, –S9 |
Name |
NaN3 |
|
Concentrations (μg/plate) |
0.5 |
||
Mean No. of colonies/plate (average of 3 ± SD) |
523 ± 20 |
NaN3: Sodium azide
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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