Methyl benzoate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 July 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: This test is comparable to guideline study and was conducted according to OECD-Guidlines for testing of chemicals, 203 Fish, Acute Toxicity Test as adopted on 04 April 1984.

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl benzoate

EC Number:

202-259-7

EC Name:

Methyl benzoate

Cas Number:

93-58-3

Molecular formula:

C8H8O2

IUPAC Name:

methyl benzoate

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): Methyl Benzoate (Benzoesauremethylester)
- Analytical purity: 98%
- Impurities (identity): 4-methylbenzaldehyde Acetophenon <2%, methyl p-toluate < 2%

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Details on test solutions:

As dilution water was reconstituted water used according to ISO/DIS 7346/1 composition. The treatment was carried out in a system consisting of two steel basin with Hostalen® lining and a capacity of 700 liters each. The dilution water solution prepared was vented to the oxygen saturation. The experiment was carried out in a static system, the calibrated to 10 liters test container consisted of glass (30 cm long, 22 cm wide, 24 cm high) and were in a water bath made ¿¿of Hostalit Zr with a visible disk of plexiglass. The test substance was mixed with dilution water with the ultra-turrax and introduced ultrasonic homogenizing and stirring with a glass rod in the experimental basin.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

The test organs were from the West Aquarium 3422 Bad Lauterberg.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21,6 - 23.0 oC for exposed fish and 21.0 - 22.4 oC for controls

pH:

6.9 -8.2 for exposed fish and 7.4 - 8.0 for controls

Dissolved oxygen:

5.9 - 9.4 mg/L for exposed fish and 7.1 9.3 for controls

Salinity:

freshwater

Nominal and measured concentrations:

0 (control), 5, 10, 25, 35, and 50 mg/l

Details on test conditions:

TEST SYSTEM
The temperature was measured with oxygen meter (OXI 196 mte) and oxygen electrode (EO 196 1 1.5). Oxygen content was measured with electronic measuring oxygen (meter oxi 196). The pH was measured with an pH meter( ph 763) electronic measurement. The test substance was mixed with dilution with ultra turrax and ultrasonic homogenizing and stir with a glass rod placed in the experimental tank. The testing solution with the exception of the 10 mg / L group, had a slight turbidity at 25 and 35 mg / L.

On presentation of the proof concentrations and subsequent measurement of water parameters per Pruf and control pool of 10 fish were used randomly. Throughout the experimental period, the fish were not fed.

After 2 -4, 24, 48, 72 and 96 hours is an inspection of the fish was the lethality and cause visible changes in the appearance and behavior were recorded and dead fish were removed from the pool. The fish was removed if no respiratory movements were more visible and he no longer even on slightly mechanical irritant response. On the same date the water parameters were measured and logged as well.

Since the factor of the square root of concentration levels 2 was no other concentrations were gepruft. Was given the experimentally determined value of the LC50 .

The holder was over a period of at least 14 days before trial begins in evaporating water planning under the following conditions.
Immediately before commencing trial was representative of the batch of fish used jeweil 10 fishing korperlange determined. these animals were not used for the experiment.

oxygen content: >80%
Temperature: 22 + or - 1 oC
light duration 12 hours day.
Stock density: < or equal to 1 gram fish/liter water
Feeding: ad Libitum

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

>= 35 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

35 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

50 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

50 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

At 5 mg /L there was protruding gill. In the concentration range of 10 to 50 mg /L fish showed the following symptoms: increased respiratory rate and respiratory movements. Activity decreased to elevated terrible reaction swim or stand at the waser upper flat and stagger on pelvic floor isolated in the side or supine position gyroscopes. The dead fish were lightened to dark discolored.

Results with reference substance (positive control):

no data

Reported statistics and error estimates:

probit analysis was not possible due to the present mortality

Any other information on results incl. tables

Sublethal observations / clinical signs:

Under the described experimental conditions BME had the following effects after 48and96 hours: Concentration                     48 hours                            48 hours                            96 hours                            96 hours (mg/l)                                   absolut                              relative (%)                       absolute                            relative (%)

0 0/20   0  0/20  0  5 0/10  0  0/10  0  10 0/10  0  0/10  0  25  0/10  0  0/10  0  35  4/10  40  5/10  50  50  10/10  100  10/10  100

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

96-hr LC50 of BME is 35 mg/l.

Executive summary:

The acute toxicity of BME of 98% purity was tested on Zebra fish (Danio rerio) for 96 hours according to OECD 203 testing guidelines. A stock solution was prepared in water and pH-adjusted to 6.9 - 8.2. The LC50 was 35 mg/L. Probit analysis was not possible due to the present mortality and the study is considered as valid with restrictions