Nickel, compound with Titanium (1:1)

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Nickel, compound with Titanium (1:1)

EC Number:

610-765-8

Cas Number:

52013-44-2

Molecular formula:

NiTi

IUPAC Name:

Nickel, compound with Titanium (1:1)

Details on test material:

100% Equiatomic NiTi intermetallic compound
Batch 1048

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

The test item, was weight in order to obtain a stock solution of 10 g/l.
Then the stock solution was diluted in order to obtain the following nominal dilutions in water (max solubility): 3.13, 0.98, 0.31 and 0.095 g/l.
Because the test item is poorly soluble in water to facilitate its solubility the stock solution has been stirred for 90 hours and then, before the beginning of the test, this solution has been ultrasonicated for 30 minutes.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Breeding conditions:
Daphnie has been kept in reconstituted water.
Lightning: 16 hrs light, 8 hrs darkness
Water change: 3 times per week

Age < 24 hrs
No. of animals: 10 for each treated sample concentration - 10 for control sample
no. of animals/beaker: 1
No. of concentration: 5

60 daphnie have been used

Study design

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

21 d

Post exposure observation period:

The number of parent immobilised organisms and/or possible abnormal behaviours both in control and in treatment group vessel and the total number of living offspring produced per parent animal alive at the end of the test have been observed. The presence of aborted eggs or dead offspring have been recorded.
Ath the beginning and at three time/week (at every test medium renewal) dissolved oxygen and pH have been measured in control and in the highest tested concentration. Temperature of the water has been recorded.

Test conditions

Hardness:

< 250 mg CaCO3/l

Test temperature:

20 +/- 2 °C

pH:

6-9

Dissolved oxygen:

> 3 mg/l

Salinity:

As dilution water, reconstituted water has been used; it has been prepared dissolving the following salts in 1 liter of deionised water.

Details on test conditions:

Because the test has been done on the extract of the test item, constituted by metals with a low volatilisation from water to air, at the beginning and at the end of the test have been checked the concentrations of two metals (nichel and titanium) in the extract by ICP-OES.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

0.95 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

immobilisation

Effect concentrations 2

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

0.31 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

immobilisation

Effect concentrations 3

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.095 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

immobilisation

Details on results:

The tested concentrations were referred to the nominal ones, because these concentrations were below the limit of quantification on ICP-OES instrument.

Any other information on results incl. tables

Total number offspring

Treated replication	Conc 10.00 g/l	Conc 3.13 g/l	Conc 0.98 g/l	Conc 0.31 g/l	Conc 0.095 g/l
1	-	-	47	54	64
2	-	-	45	55	68
3	-	-	39	53	70
4	-	-	43	64	71
5	-	-	42	60	70
6	-	-	-	57	68
7	-	-	35	61	79
8	-	-	-	54	70
9	-	-	-	58	69
10	-	-	-	-	62

Treated-immobilized parent daphnie (death day)

Treated replication	Conc 10.00 g/l	Conc 3.13 g/l	Conc 0.98 g/l	Conc 0.31 g/l	Conc 0.095 g/l
1	26/8/13	26/8/13	-	-	-
2	26/8/13	26/8/13	-	-	-
3	26/8/13	26/8/13	-	-	-
4	26/8/13	27/8/13	-	-	-
5	26/8/13	26/8/13	-	-	-
6	26/8/13	27/8/13	06/9/13	-	-
7	26/8/13	26/8/13	-	-	-
8	26/8/13	27/8/13	07/09/13	-	-
9	26/8/13	26/8/13	06/09/13	-	-
10	26/8/13	27/8/13	08/09/13	07/09/13	-

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

On the basis of obtained results, according to Official Journal of the European Union 1272/2008 (CLP) and OECD no211, the test item has a LOEC of 0.31 g/l and a NOEC of 0.095 g/l after 21 days. .