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EC number: 700-527-2 | CAS number: 1271488-66-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 November - 6 December 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted by GLP accredited laboratory. Method according to OECD guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- yes
- Remarks:
- Temporary deviations from the minimal level of temperature and relative humidity. Laboratory historical data do not indicate an effect of the deviations and the study integrity was not adversely affected.
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.42 (Skin Sensitisation: Local Lymph Node Assay)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.2600 (Skin Sensitisation)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- methyl trans-3-oxo-2-pentylcyclopentanecarboxylate
- EC Number:
- 700-527-2
- Cas Number:
- 1271488-66-4
- Molecular formula:
- C12H20O3
- IUPAC Name:
- methyl trans-3-oxo-2-pentylcyclopentanecarboxylate
- Details on test material:
- - Name of test material (as cited in study report): Yasmolys
- Substance type: pure active substance
- Physical state: liquid
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- The animals were CBA/J strain mice (inbred, SPF quality) from Janvier, Le Genest-Saint-Isle, France. Animals ±9 weeks old were selected having body weights +/-20% of the sex mean. They were identified at the tail with a marker pen. Special attention during health inspection was paid to the ears, whcih were intact and free from any abnormality.
A controlled environment was maintained in the room with optimal conditions of approximately 15/h air changes, a temperature of 17.9-22.2ºC, a relative humidity of 21-61% and a 12 hour artificial fluorescent light/12 hour dark cycle per day.
3 animals were present per cage in labeled Macrolon cages containing sterilised sawdust as bedding material and paper as cage-enrichment. Animals were acclimitised for a period of 5 days prior to exposure to the test substance. The animals had free access to tap water and pelleted rodent diet (SM R/M-Z from SSNIFF® Spezialdiäten GmbH, Soest , Germany.
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 0, 25, 50, 100% test substance in vehicle
- No. of animals per dose:
- 5 animals per dose group (4 dose groups)
- Details on study design:
- Pre-screen test
50% and 100% test substance in the vehicle were daily applied on a mouse (2 mice per concentration) for 3 consecutive days. The highest concentration should cause no systemic toxicity and have a maximum irritation score of 2.
Main study
Three groups of five animals were daily topically treated at the dorsal of both ears with one dose level per group for three consecutive days (Day 1, 2 and 3). The negative control animals were treated in the same way with the vehicle only.
On Day 6, the animals were injected at their tail veins with 0.25 ml sterile phosphate buffer (PBS) containing 20 µCi 3H-methyl thymidine. After 5 hours, the animals were sacrificed and the draining auricular lymph node was excised. The relative sizes of the nodes were visually examined and abnormalities recorded. The nodes were pooled for each animal.
A single cell suspension of lymph node cells (LNC) was prepared by mechanical disaggregation through a 125 µm in diameter stainless steel gauze. Cells are washed with PBS and centrifuged at 200g for 10 minutes at 4°C. The DNA was precipitated by exposing the LNC to 5% trichloro acetic acid (TCA) until the next day.
Precipitates were recovered by centrifugation and resuspended in 1ml TCA.
Radio activity was measured by a scintillation counter as expressed in disintegration per minute (dpm).
The stimulation index (SI) is the ratio of dpm of each dose group to the dpm of the vehicle control group. It was calculated for each dose group. - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
Results and discussion
- Positive control results:
- Hexyl cinnamaldehyde (CAS 101-86-0) was used as a positive control. The positive control test was performed in September 2010 on female mice of the CBA/J strain. The hexyl cinnamaldehyde concentrations were 0, 5, 10 and 25%. The disintergrations per minute increase over that range from 678 to 2502. the corresponding stimulation indici increased from 1 to 3.7. On this basis, an EC3 of 18% was calculated and fitted into the historical range of 2-20%. The results of the last six-months reliability checks were 13.8, 13.9, 16.0, 11.9, 16.9 and 10.7%. It was concluded that the results were reproducible and accurate.
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: see Remark
- Remarks:
- The stimulation index for each test substance concentration, was the ratio of the mean radio activity for that concentration divided by the mean radio activity level of the vehicle control. The stimulation index was coorelated with the concentration of the test substance and increased from 0.9 to 1.9 when the concentration was increased from 25 to 100%. In case of application of the undiluted test substance to the dorsal ear surface of the mouse, the stimulation index remained below 3.
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: see Remark
- Remarks:
- The nodes were pooled for each animal and the mean radio-activity value measured (Table 3). The values were expressed in disintegrations per minute and the mean radio activity levels were averaged for each test substance concentration. From a test substance concentration of 25% to 100% (undiluted), the radio activity increased from 464 to 975 dpm. The vehicle control was 523 dpm.
Any other information on results incl. tables
Main test
The body weights in Table 1 are in the same range as the historical controls over the study period. The slight body weight loss noted in some animals was considered not toxicological significant.
Table 1 Body weight and relative size auricular lymph nodes of mice subjected to the test substance dissolved in acetone/olive oil in the main test
Concentration test substance | Day 1 | size node | |||
Animal | bw (g) | bw (g) | left | right | |
0% | 1 | 24 | 22 | n | n |
2 | 21 | 21 | n | n | |
3 | 21 | 21 | n | n | |
4 | 23 | 22 | n | n | |
5 | 22 | 23 | n | n | |
25% | 1 | 23 | 23 | n | n |
2 | 22 | 22 | n | n | |
3 | 22 | 22 | n | n | |
4 | 22 | 21 | n | n | |
5 | 22 | 22 | n | n | |
50% | 1 | 21 | 21 | n | n |
2 | 21 | 21 | n | n | |
3 | 23 | 23 | n | n | |
4 | 21 | 21 | n | n | |
5 | 21 | 21 | n | n | |
100% | 1 | 22 | 22 | n | n |
2 | 21 | 21 | n | n | |
3 | 21 | 22 | n | n | |
4 | 21 | 21 | n | n | |
5 | 22 | 22 | n | n | |
bw: body weight; n: normal |
The skin reactions are tabulated in Table 2. Slight erythema of the ears was observed at a concentration level of 50% and 100% between days 2 and 3. It was considered not to have any toxicological significance on the activity of the nodes. No oedema was observed in any of the animals. All auricular lymph nodes of the animals were considered normal in size (Table 2). No macroscopic abnormalities of the surrounding area were noted in any of the animals.
Table2 Erythema (E) and Oedema (O) on the dorsal surface of the left and right ear of the mice exposed to the test substance in the main test
Concentration test substance | Day 1 | Day 2 | Day 3 | Day 4 | Day 5 | Day 6 | |||||||||||||||||||
Animal | left | right | left | right | left | right | left | right | left | right | left | right | |||||||||||||
E | O | E | O | E | O | E | O | E | O | E | O | E | O | E | O | E | O | E | O | E | O | E | O | ||
0% | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
25% | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
50% | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
3 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
4 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
5 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
100% | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
3 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
4 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
5 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Erythema score: 0 (no erythema) - 4 (severe erythema to eschar formation)
Oedema score: 0 (no oedema) - 3 (severe oedema)
Table 3 Radio-activity measured in the individual animals
Concentration test substance | DPM per animal | |
Animal | ||
0% | 1 | 518 |
2 | 649 | |
3 | 350 | |
4 | 619 | |
5 | 480 | |
25% | 1 | 435 |
2 | 496 | |
3 | 175 | |
4 | 604 | |
5 | 608 | |
50% | 1 | 340 |
2 | 407 | |
3 | 533 | |
4 | 1042 | |
5 | 792 | |
100% | 1 | 700 |
2 | 792 | |
3 | 958 | |
4 | 1328 | |
5 | 1098 | |
DPM: disintegrations per minute |
Table 4 Mean disintegrations per minute (DPM) and stimulation index (SI), including the standard error of the mean (SEM) calculated from the radio activity measurement in the animals subjected to the test concentration.
Concentration test substance | mean | mean | ||
DPM | SEM | DPM | SEM | |
0% | 523 | 53 | 1 | 0.2 |
25% | 464 | 79 | 0.9 | 0.3 |
50% | 623 | 130 | 1.2 | 0.3 |
100% | 975 | 112 | 1.9 | 0.1 |
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information
- Conclusions:
- The six-month reliability check with the positive control proved that this LLNA was appropiate for testing contact hypersensitivity.
Since there was no indication that the test substance applied undiluted at the dorsal surface of the mouse ear elicits a stimulation index SI≥3, the test substance is regarded as a non skin sensitiser. - Executive summary:
The contact hyper sensitivity of the test substance was assessed by the Local Lymph Node Assay conducted according to the OECD and EU test guidelines. Four experimental groups of five female CBA/J mice were treated with the test substance at concentrations of 0 (vehicle control), 25, 50 and 100%. The animals were visually scored for skin irritation reactions. Three days after the last exposure, all animals were injected with tritiated methyl thymidine. The auricular lymph nodes were isolated and their radio activity levels determined. The stimulation index was subsequently calculated. The positive control hexyl cinnamic aldehyde was included as a six-months reliability check, but not as a concurrent positive control group.
Slight erythema of the ears was observed at the animals treated with 50 and 100% of the test substance between days 2 and 3. No oedema was observed.
The stimulation index showed a clear dose-response relationship, but it remained below three for the undiluted test substance. The index was 1.9 and the test substance is therefore not regarded as a skin sensitiser.
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