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Diss Factsheets
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EC number: 611-084-9 | CAS number: 54041-17-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Type: mixed population of aquatic microorganisms (activated sludge)
- Origin: laboratory scale sewage treatment unit
- Pre-Treatment: None - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Nominal and measured concentrations:
- 560, 1000, 1800, 3200 and 5600 mg/L (nominal)
- Details on test conditions:
- - Test item concentration/s: 560, 1000, 1800, 3200 and 5600 mg/L
- Test item concentration in physico-chemical oxygen consumption control: 10000 mg/L
The test item concentrations were not confirmed by analytical methods, they were based on nominal concentrations.
- Method of application: direct weighings
- Test concentration of the activated sludge: 435 mg/L
- Test temperature: 20 ± 2°C
- Incubation time: 3 hours with permanent aeration - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 760 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- EC50 of the reference compound 3,5-Dichlorophenol is in the range 5 – 30 mg/L (13 mg/L).
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 3h-EC50 of 1760 mg/L was determined for the test substance towards microorganisms.
- Executive summary:
A study was conducted to assess the toxicity of the test substance to bacteria in accordance with ISO 8192 ”Activated sludge respiration inhibition” (1986). This test method is equal to OECD Guideline 209 (1984).
The activated sludge was exposed to the test substance at different concentrations (560, 1000, 1800, 3200 and 5600 mg/L).The respiration rate of each mixture was determined after aeration periods of 3 hours.
A 3h-EC50 of 1760 mg/L was determined for the test substance towards microorganisms. The effect value related to nominal concentration, since no analytical monitoring was performed.
For the reference compound 3,5-Dichlorophenol an EC50 of 13 mg/L was determined.
Reference
Inhibition
concentrations [mg/L] | respiration rate [mg/L*h] | inhibition [%] |
5600 | 4.3 | 80.9 |
3200 | 6.0 | 73.3 |
1800 | 11.1 | 50.7 |
1000 | 16.5 | 26.7 |
560 | 18.0 | 20.0 |
control | 22.5 |
Description of key information
A study was conducted to assess the toxicity of the test substance to bacteria in accordance with ISO 8192 ”Activated sludge respiration inhibition” (1986). This test method is equal to OECD Guideline 209 (1984).
The activated sludge was exposed to the test substance at different concentrations (560, 1000, 1800, 3200 and 5600 mg/L).The respiration rate of each mixture was determined after aeration periods of 3 hours.
A 3h-EC50 of 1760 mg/L was determined for the test substance towards microorganisms.The effect value related to nominal concentration, since no analytical monitoring was performed.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 760 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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