Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 682-238-0 | CAS number: 1190931-27-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 6 October 2009 to 26 February 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.6 (Skin Sensitisation)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- Buehler test
- Justification for non-LLNA method:
- The test item cC6O4 ammonium salt is an anionic surfactant classified according to CLP Regulation (REGULATION (EC) No 1272/2008) as Skin Irritant 2.
The LLNA test indicates C6O4 ammonium salt as skin sensitizer.
However, as expressed in the OECD 429 guideline the LLNA may not be suitable to test irritants.
In scientific literature, the fact that irritancy augments the induction response and/or can sometimes lead to non-specific proliferation responses in the LLNA is known (Basketter et al., 2009;Montelius et al., 1998; Woolhiser et al., 1998; Basketter et al., 2007a,b,c).
This raises the possibility of false positive results in the original LLNA caused by non-specific cell activation as a result of inflammatory processes in the skin (irritation) (Vohr and Ahr, 2005).
The possibility of false positive findings for irritant substances, including some type of surfactants, is reported in the OECD guideline 429.
Mehling et al (2007)reports that for the category of surfactants, which is generally regarded to be without a sensitization, unexpected positive results were observed in LLNA tests.The OECD 429 guideline indicates as limitation the use of LLNA for this category of chemicals.
Basing on the reasonings reported above and according to the OECD 429 guideline, which states it should be recognised that for classes substances that are potential confounders the use of OECD 406 may necessitate, it was deemed necessary to better assess the sensitization potential of the test item carrying out a further study.
A Buehler test (OECD 406) was performed in order to get reliable information on skin sensitizer properties of the test substance.
Test material
- Reference substance name:
- cC6O4 ammonium salt
- IUPAC Name:
- cC6O4 ammonium salt
- Reference substance name:
- Acetic acid, 2,2-difluoro-2-[[2,2,4,5- tetrafluoro-5-(trifluoromethoxy)-1,3- dioxolan-4-yl]oxy]-, ammonium salt (1:1)
- IUPAC Name:
- Acetic acid, 2,2-difluoro-2-[[2,2,4,5- tetrafluoro-5-(trifluoromethoxy)-1,3- dioxolan-4-yl]oxy]-, ammonium salt (1:1)
- Reference substance name:
- ammonium 2,2-difluoro-2-{[2,2,4,5-tetrafluoro-5-(trifluoromethoxy)-1,3-dioxolan-4-yl]oxy}acetate
- EC Number:
- 682-238-0
- Cas Number:
- 1190931-27-1
- Molecular formula:
- C6H4F9NO6
- IUPAC Name:
- ammonium 2,2-difluoro-2-{[2,2,4,5-tetrafluoro-5-(trifluoromethoxy)-1,3-dioxolan-4-yl]oxy}acetate
- Details on test material:
- - Name of test material (as cited in study report): C6O4 cyclic
- Substance type: pure substance (dry salt)
- Physical state: white powder
- Analytical purity: 98.2%
- Impurities (identity and concentrations): NH4F= 0.02% (w/w), NH4Cl=0.25% (w/w), H2O=0.2% (w/w), Organic impurities= 1.3% (w/w)
- Composition of test material, percentage of components: not reported
- Isomers composition: not reported
- Purity test date: not reported
- Lot/batch No.: 156/17
- Expiration date of the lot/batch: 31 December 2020
- Stability under test conditions: not reported
- Storage condition of test material: room temperature
Constituent 1
Constituent 2
Constituent 3
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- Dunkin-Hartley
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Supplier: Charles River Italia S.p.A., Calco (Lecco), Italy. Breeder: Charles River Germany
- Age at study initiation: 4-5 weeks old
- Weight at study initiation: 271 to 325 grams
- Housing: Stainless steel cages measuring 48x63x41 (during study) or 87x71x24 cm (during acclimatisation), with grid floor. Daily inspected and changed as necessary (at least 3 times/week)
- Diet (e.g. ad libitum): 8GP17 (Mucedola S.r.l., Via G. Galilei, 4, 20019, Settimo Milanese (MI) Italy) ad libitum
- Water (e.g. ad libitum): drinking water supplied to each cage via a water bottle (ad libitum)
- Acclimation period: At least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22°C ± 2°C
- Humidity (%): 55% ± 15%
- Air changes (per hr): Approximately 15 to 25 air changes per hour
- Photoperiod (hrs dark / hrs light): Artificial (fluorescent tubes), daily light/dark cycle of 12/12 hours
IN-LIFE DATES:
From: no data
To: the end of the experimental procedure.
Study design: in vivo (non-LLNA)
Induction
- Route:
- epicutaneous, occlusive
- Vehicle:
- water
- Concentration / amount:
- MAIN Study - Induction:
50% test substance in sterile water - Day(s)/duration:
- day 1-3 , day 8-10 and day 15-17
Challenge
- No.:
- #1
- Route:
- epicutaneous, occlusive
- Vehicle:
- water
- Concentration / amount:
- MAIN Study - Challenge:
50% test substance in sterile water - Day(s)/duration:
- On day 29
- No. of animals per dose:
- PRELIMINARY SCREEN:
5 animals. Each animal dosed with 2 concentration of the test item.
MAIN STUDY:
test group: 20 animal
control group: 10 animals - Details on study design:
- The study was divided into 2 distinct phases. The first of these consisted of a preliminary screen which was used to determine suitable test item concentrations to be used in the second phase. This second phase constituted the main study: the determination of the sensitisation potential of the test item.
Allocation to groups:
Animals were allocated to treatment groups prior to each phase of the study.
Preliminary screen:
Five animals were selected from those available and the flanks clipped free of hair. Each animal was dosed with 2 concentrations of the test item, 1 on either flank. A gauze patch measuring at least 20x20 mm was soaked with 0.4 ml of the selected concentration of the test item. This was then placed onto the selected treatment site. A strip of synthetic film was placed over the treated sites and the whole assembly was secured in position by encircling the trunk of the animal with a length of adhesive strapping.
All animals were treated in this manner such that a total of 5 concentrations (50, 20, 10, 5 and 1% in the selected vehicle, sterile water) of the test item were dosed each in duplicate. The adhesive strapping and patches were removed after approximately 6 hours contact with the skin. The treated sites were washed with lukewarm water to remove any remaining test item.
Approximately 24 and 48 hours after removal of the dressings, the treated sites were examined for signs of reaction to treatment. Each site was assessed and scored using the following scale:
No visible change : 0
Discrete or patchy erythema: 1
Moderate and confluent erythema: 2
Intense erythema and swelling: 3
Main study - Induction:
Animals were allocated to treatment to give a test group of 20 animals and a control group of 10 animals.
On the day of dosing (Day 1) the hair was clipped from the anterior region of the left flank of each animal. Animals of the test group were treated with the test item at a concentration of 50%. A gauze patch measuring 20x20 mm was soaked with 0.4 ml of the test item and placed onto the selected skin site. A strip of synthetic film was placed over the treated sites and the whole assembly was secured in position by encircling the trunk of the animal with a length of adhesive strapping. All animals of the test group were treated with the test item in this manner and animals of the control group were similarly treated with the selected vehicle (sterile water).
After an exposure period of 6 hours the dressings were removed. The treated sites were cleaned of remaining test item or vehicle by washing with lukewarm water.
Approximately 24 and 48 hours after removal of the patches the treated sites were examined for signs of reaction to treatment. Each site was assessed and scored using the following scale:
No visible change : 0
Discrete or patchy erythema: 1
Moderate and confluent erythema: 2
Intense erythema and swelling: 3
These procedures were repeated at weekly intervals (Days 8 to 10 and 15 to 17 of the study).
Main study - Challenge
On Day 29, the hair was removed with electric clippers from both the anterior and posterior regions of the right flank of all animals of both test and control groups.
A 0.4 ml aliquot of the test item at a concentration of 50% was spread evenly over an absorbent patch measuring approximately 20x20 mm. This was placed onto the skin of the posterior region of the prepared site on the right flank. A similar patch, containing 0.4 ml of the vehicle selected for the challenge (sterile water), was placed onto the anterior region of the prepared site. A strip of synthetic film was placed over the treated sites and the whole assembly was secured in position by encircling the trunk of the animal with a length of adhesive strapping. All animals of the test and control groups were treated with both the test item and vehicle in this manner. After an exposure period of approximately 6 hours the dressings were removed and the treated sites cleaned of the remaining test item by washing with lukewarm water.
Approximately 21 hours after removal of the dressing and patches, the treated sites were closely clipped to remove any hair that may have grown. Approximately 3 hours later, 24 hours after removal of the dressing, the treated sites were examined for any signs of reaction to treatment.
The degree of skin reaction was scored according to the following scheme:
No visible change : 0
Discrete or patchy erythema: 1
Moderate and confluent erythema: 2
Intense erythema and swelling: 3
Skin reaction on the treated sites was again assessed approximately 24 hours after the first examination (approximately 48 hours after removal of the patches).
Termination and necropsy:
All animals were killed by carbon dioxide narcosis following the end of the experimental procedure. No necropsy examination was performed on these animals. - Challenge controls:
- The same concentration of test item (50% in sterile water) was selected for the challenge as no irritation was noted in all main phase animals.
No response was observed to the test item at the selected concentration, in either test or control group animals 24 and 48 hours following 6 hours topical exposure. No reaction was observed to the vehicle alone. - Positive control substance(s):
- yes
- Remarks:
- A positive control check using alpha-Hexylcinnamaldehyde is performed approximately at 6 monthly intervals.
Results and discussion
- Positive control results:
- A positive control check using alpha-Hexylcinnamaldehyde is performed approximately at 6 monthly intervals.
A summary relevant to the most recent reliability check (RTC STUDY NUMBER: 28130-007INT) performed follows:
REFERENCE SUBSTANCE: α-HEXYLCINNAMALDEHYDE
CONCENTRATION INDUCTION: 70% in DMSO
CONCENTRATION CHALLENGE: 15% in acetone
CRITICAL DATES:
INDUCTION: 23 March 2009, 30 March 2009, 06 April 2009
CHALLENGE: 20 April 2009
RESULTS: 20 % response in test group and 0 % response in control group at challenge
INTERPRETATION: Incidence at challenge acceptable
Test system regarded as valid.
In accordance with OECD TG 406 the reliability check of the model to confirm sensitivity and reliability of the technique was performed every 6 month with a positive control substance (Alpha-Hexylcinnamaldehyde). The number of animals tested with the positive control were not reported, but were assumed to follow recommendations for positive control (minimum 10 animals for control groups).
In vivo (non-LLNA)
Resultsopen allclose all
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 0.4 ml of sterile water
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Clinical observations:
- no reported effects
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- 0.4 ml of sterile water
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Clinical observations:
- no reported effects
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 0.4 ml of a 50% aqueous solution
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- no reported effects
- Key result
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 0.4 ml of a 50% aqueous solution
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- no reported effects
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- positive control
- Dose level:
- 15%
- No. with + reactions:
- 2
- Total no. in group:
- 10
- Clinical observations:
- no reported effects
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- positive control
- Dose level:
- 15 %
- No. with + reactions:
- 2
- Total no. in group:
- 10
- Clinical observations:
- no reported effects
Any other information on results incl. tables
This table details the findings at the treated sites on the left flank of each animal 24 and 48 hours following 6 hours topical exposure to the test item, C6O4 cyclic, at a concentration of 50% and the vehicle alone (sterile water) during the induction procedure:
Group function
|
Animal number |
Dermal Response |
|||||
First Induction |
Second Induction |
Third Induction |
|||||
24 hours |
48 hours |
24 hours |
48 hours |
24 hours |
48 hours |
||
CONTROL |
181 |
0 |
0 |
0 |
0 |
0 |
0 |
183 |
0 |
0 |
0 |
0 |
0 |
0 |
|
185 |
0 |
0 |
0 |
0 |
0 |
0 |
|
187 |
0 |
0 |
0 |
0 |
0 |
0 |
|
189 |
0 |
0 |
0 |
0 |
0 |
0 |
|
191 |
0 |
0 |
0 |
0 |
0 |
0 |
|
193 |
0 |
0 |
0 |
0 |
0 |
0 |
|
195 |
0 |
0 |
0 |
0 |
0 |
0 |
|
197 |
0 |
0 |
0 |
0 |
0 |
0 |
|
199 |
0 |
0 |
0 |
0 |
0 |
0 |
|
TEST |
201 |
0 |
0 |
0 |
0 |
0 |
0 |
203 |
0 |
0 |
0 |
0 |
0 |
0 |
|
205 |
0 |
0 |
0 |
0 |
0 |
0 |
|
207 |
0 |
0 |
0 |
0 |
0 |
0 |
|
209 |
0 |
0 |
0 |
0 |
0 |
0 |
|
211 |
0 |
0 |
0 |
0 |
0 |
0 |
|
213 |
0 |
0 |
0 |
0 |
0 |
0 |
|
215 |
0 |
0 |
0 |
0 |
0 |
0 |
|
217 |
0 |
0 |
0 |
0 |
0 |
0 |
|
219 |
0 |
0 |
0 |
0 |
0 |
0 |
|
221 |
0 |
0 |
0 |
0 |
0 |
0 |
|
223 |
0 |
0 |
0 |
0 |
0 |
0 |
|
225 |
0 |
0 |
0 |
0 |
0 |
0 |
|
227 |
0 |
0 |
0 |
0 |
0 |
0 |
|
229 |
0 |
0 |
0 |
0 |
0 |
0 |
|
231 |
0 |
0 |
0 |
0 |
0 |
0 |
|
233 |
0 |
0 |
0 |
0 |
0 |
0 |
|
235 |
0 |
0 |
0 |
0 |
0 |
0 |
|
237 |
0 |
0 |
0 |
0 |
0 |
0 |
|
239 |
0 |
0 |
0 |
0 |
0 |
0 |
KEY: 0 = No visible change
1 = Discrete or patchy erythema
2 = Moderate and confluent erythema
3 = Intense erythema and swelling
This table details the findings at the treated sites on the right flank of each animal 24 and 48 hours following challenge by 6 hours topical exposure to the test item, C6O4 cyclic, at a concentration of 50% and the vehicle alone (sterile water):
Group function
|
Animal number |
Dermal Response |
|||
Vehicle |
Test item |
||||
24 hours |
48 hours |
24 hours |
48 hours |
||
CONTROL |
181 |
0 |
0 |
0 |
0 |
183 |
0 |
0 |
0 |
0 |
|
185 |
0 |
0 |
0 |
0 |
|
187 |
0 |
0 |
0 |
0 |
|
189 |
0 |
0 |
0 |
0 |
|
191 |
0 |
0 |
0 |
0 |
|
193 |
0 |
0 |
0 |
0 |
|
195 |
0 |
0 |
0 |
0 |
|
197 |
0 |
0 |
0 |
0 |
|
199 |
0 |
0 |
0 |
0 |
|
TEST |
201 |
0 |
0 |
0 |
0 |
203 |
0 |
0 |
0 |
0 |
|
205 |
0 |
0 |
0 |
0 |
|
207 |
0 |
0 |
0 |
0 |
|
209 |
0 |
0 |
0 |
0 |
|
211 |
0 |
0 |
0 |
0 |
|
213 |
0 |
0 |
0 |
0 |
|
215 |
0 |
0 |
0 |
0 |
|
217 |
0 |
0 |
0 |
0 |
|
219 |
0 |
0 |
0 |
0 |
|
221 |
0 |
0 |
0 |
0 |
|
223 |
0 |
0 |
0 |
0 |
|
225 |
0 |
0 |
0 |
0 |
|
227 |
0 |
0 |
0 |
0 |
|
229 |
0 |
0 |
0 |
0 |
|
231 |
0 |
0 |
0 |
0 |
|
233 |
0 |
0 |
0 |
0 |
|
235 |
0 |
0 |
0 |
0 |
|
237 |
0 |
0 |
0 |
0 |
|
239 |
0 |
0 |
0 |
0 |
KEY: 0 = No visible change
1 = Discrete or patchy erythema
2 = Moderate and confluent erythema
3 = Intense erythema and swelling
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- No response to the test item, at the selected concentration, was apparent at challenge in any animal of the test and control groups.
These results indicate that the test item cC6O4 ammonium salt does not elicit a sensitisation response in the guinea pig, being there no evidence of response at challenge following a period of induction exposure to the test item. - Executive summary:
The potential of the test item, cC6O4 ammonium salt, to induce and elicit delayed dermal sensitisation was assessed by a guinea pig model using the methods of Buehler.
The concentrations of the test item used in the main study were determined by the results of a preliminary screening test. The main sensitisation test was undertaken using a test group of 20 animals and a control group of 10 animals. In an attempt to induce sensitisation, test animals were treated by topical application of the test item at a concentration of 50% in sterile water. This was repeated at weekly intervals for a total of 3 weeks. Animals of the control group were treated in the same manner but the vehicle alone (sterile water) was used in place of the test item. Two weeks after the third and final induction exposure, animals of the test and control groups were challenged by topical application of both the test item and the vehicle alone (sterile water).
At challenge no response was observed for the test item at a concentration of 50% in either test or control group animals. No reaction was observed to the vehicle alone (sterile water).
These results indicate that the test item cC6O4 ammonium salt does not elicit a sensitisation response in the guinea pig, being there no evidence of response at challenge following a period of induction exposure to the test item.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.