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EC number: 298-791-2 | CAS number: 93839-21-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-10-09 to 2009-10-16
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-[4-(aminocarbonyl)phenyl]-4-nitrobenzamide
- EC Number:
- 298-791-2
- EC Name:
- N-[4-(aminocarbonyl)phenyl]-4-nitrobenzamide
- Cas Number:
- 93839-21-5
- Molecular formula:
- C14H11N3O4
- IUPAC Name:
- N-(4-carbamoylphenyl)-4-nitrobenzamide
- Details on test material:
- - Name of test material (as cited in study report): p-Nitrobenzoylaminobenzamid trocken
- Molecular formula: C14H11N3O4
- Molecular weight: 285.26 g/mol
- Physical state: Solid, white powder
- Expiration date of the lot/batch: 2010-10-01
- Storage condition of test material: Room temperature, protected from moisture and light.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The concentrations of the test item were analytically determined from all test concentrations and the control after 0 h (test start) and 72 h (test end).
- Sampling method: Samples at start and end of the test were taken directly from the test replicates.
- Sample storage conditions before analysis: All samples were stored at 6 ± 2 °C until start of analysis, if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A saturated solution was prepared. A dispersion of 66 mg/L prepared in dilution water was shaken with 20 rpm for 48 h at ambient temperature and filtrated with 0.45 µm RC filter.
- Eluate: Dilution water
- Differential loading: The saturated solution and further four dilution levels were tested in a geometrical series with a dilution factor 2: 6.25 - 12.5 - 25 - 50 and 100 % of the saturated solution corresponding to initial measured concentrations of 0.0157 – 0.0314 – 0.0712 – 0.153 – 0.304 mg/L.
- Controls: Six replicates without test item were tested under the same test conditions as the test replicates.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus Chodat
- Strain: SAG 86.81
- Source (laboratory, culture collection): SAG Pflanzenphysiologisches Institut der Universitaet Goettingen, Nikolausberger Weg 18, D-37073 Goettingen, Germany
- Age of inoculum (at test initiation): 4 days
- Method of cultivation: Fresh stocks were prepared from Z-Agar. Light intensity amounted 35 - 70 µE x m-2 x s-1 for 24 h per day.
ACCLIMATION
- Culturing media and conditions (same as test or not): Same as test
- Any deformed or abnormal cells observed: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- Not measured
- Test temperature:
- Mean: 22.5 °C, Min: 22 °C, Max: 23 °C
- pH:
- Initial measured test item concentration pH-value
[mg/L] Start; 0 h End; 72 h
0.304 8.09 8.72
0.153 8.17 8.34
0.0712 8.13 8.36
0.0314 8.07 8.23
0.0157 8.16 8.43
Control 7.97 8.49 - Dissolved oxygen:
- Not measured
- Salinity:
- Not measured, freshwater
- Nominal and measured concentrations:
- Please refer to "any other information on materials and methods incl. tables"
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: sterile 250 mL Erlenmeyer flasks, test volume 100 mL
- Aeration: Test containers were placed on a rotary shaker and oscillated at appr. 70 rpm
- Type of flow-through (e.g. peristaltic or proportional diluter): None
- Renewal rate of test solution (frequency/flow rate): One application at test start
- Initial cells density: 5074 cells/mL
- Control end cells density: Mean 873651 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): None
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water, composition acc. to the guideline
- Hardness: 0.24 mmol Ca+Mg/L
- pH-value: 8.1 +/- 0.2
- Culture medium: Nutrient Medium Z acc. to LÜTTGE et al. (1994)
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 24 h
- Light intensity and quality: 60 - 120 µE x m-2 x s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] Fluorimeter, via Chlorophyll-a fluorescence (excitation at 436 nm, emission at 685 nm), cell concentrations were related to all cell density values acc. to a calibration curve.
TEST CONCENTRATIONS
- Range finding study: Yes
Results of the Range Finding Test (0-72 h)
Results of the Range Finding Test (0-72 h)
Dilution level of the saturated solution
[%] Rate Related Inhibition[%] Yield Inhibition[%]
100 29 68
10 -2 -8
1 1 3 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.304 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks:
- and inhibition of yield
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Other: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50:
Rate-related inhibition: 0.600 (CI 0.545 - 0.653) mg/L
Yield Inhibition: 0.280 (CI 0.266 - 0.299) mg/L - Reported statistics and error estimates:
- EC10-, EC20- and EC50-values of the growth rate and yield inhibition after 72 h were calculated by sigmoidal dose-response regression. Calculation of the confidence intervals EC10-, EC20- and EC50-values were carried out using standard procedures.
NOEC/LOEC was determined by calculation of statistical significance of growth rates and yield. One Way Analysis of Variance (ANOVA) and DUNNETT’s test was used for NOEC/LOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-values for both Normality and Equal Variance test are 0.05. The a-value (acceptable probability of incorrectly concluding that there is a difference) was a=0.05.
Any other information on results incl. tables
NOEC, LOEC and EC - values of p-Nitrobenzoylaminobenzamid trocken (0-72 h)
based on initial measured test item concentrations [mg/L]
Rate-related Inhibition |
|
NOEC |
0.304 |
LOEC |
> 0.304 |
ErC10 |
> 0.304 |
ErC20 |
> 0.304 |
ErC50 |
> 0.304 |
Inhibition of Yield |
|
NOEC |
0.304 |
LOEC |
> 0.304 |
EyC10 |
> 0.304 |
EyC20 |
> 0.304 |
EyC50 |
> 0.304 |
Cell Densities
|
Initial measured test item concentration |
Replicate |
Cell density [cells/mL] |
||||||
[mg/L] |
No. |
0 h |
24 h |
48 h |
72 h |
|
|||
0.304 |
1 |
5074 |
28858 |
207909 |
925614 |
|
|||
2 |
5074 |
21790 |
170427 |
897132 |
|
||||
3 |
5074 |
23864 |
195671 |
1091558 |
|
||||
Mean |
5074 |
24837 |
191336 |
971435 |
|
||||
0.153 |
1 |
5074 |
28444 |
223420 |
948956 |
|
|||
2 |
5074 |
28952 |
179422 |
937676 |
|
||||
3 |
5074 |
30617 |
197827 |
1029516 |
|
||||
Mean |
5074 |
29338 |
200223 |
972049 |
|
||||
0.0712 |
1 |
5074 |
22860 |
186282 |
896820 |
|
|||
2 |
5074 |
26201 |
181313 |
921387 |
|
||||
3 |
5074 |
31031 |
210714 |
1085682 |
|
||||
Mean |
5074 |
26697 |
192770 |
967963 |
|
||||
0.0314 |
1 |
5074 |
24754 |
145192 |
837169 |
|
|||
2 |
5074 |
27341 |
172916 |
940592 |
|
||||
3 |
5074 |
28526 |
182383 |
935893 |
|
||||
Mean |
5074 |
26874 |
166830 |
904551 |
|
||||
0.0157 |
1 |
5074 |
27522 |
150722 |
870014 |
|
|||
2 |
5074 |
25480 |
167327 |
976901 |
|
||||
3 |
5074 |
32232 |
216585 |
1077290 |
|
||||
Mean |
5074 |
28411 |
178211 |
974735 |
|
||||
Control |
1 |
5074 |
23532 |
184998 |
824193 |
|
|||
2 |
5074 |
26312 |
182768 |
824656 |
|
||||
3 |
5074 |
27788 |
204904 |
1002731 |
|
||||
4 |
5074 |
24615 |
171239 |
781946 |
|
||||
5 |
5074 |
30383 |
181964 |
843692 |
|
||||
6 |
5074 |
23942 |
183375 |
964687 |
|
||||
Mean |
5074 |
26095 |
184875 |
873651 |
|
Evaluation after 72 h
Initial measured test item concentration |
Replicate |
Growth rate |
Rate-related inhibition |
Yield |
Inhibition of yield |
||
[mg/L] |
No. |
[d-1] |
[%] |
[cells/mL] |
[%] |
||
0.304 |
1 |
1.74 |
-1.20 |
920540 |
-5.98 |
||
2 |
1.73 |
-0.60 |
892058 |
-2.70 |
|||
3 |
1.79 |
-4.41 |
1086484 |
-25.1 |
|||
Mean |
1.75 |
-2.07 |
966361 |
-11.3 |
|||
0.153 |
1 |
1.74 |
-1.69 |
943882 |
-8.67 |
||
2 |
1.74 |
-1.46 |
932602 |
-7.37 |
|||
3 |
1.77 |
-3.27 |
1024442 |
-17.9 |
|||
Mean |
1.75 |
-2.14 |
966975 |
-11.3 |
|||
0.0712 |
1 |
1.73 |
-0.59 |
891746 |
-2.67 |
||
2 |
1.73 |
-1.11 |
916313 |
-5.50 |
|||
3 |
1.79 |
-4.30 |
1080608 |
-24.4 |
|||
Mean |
1.75 |
-2.00 |
962889 |
-10.9 |
|||
0.0314 |
1 |
1.70 |
0.75 |
832095 |
4.20 |
||
2 |
1.74 |
-1.52 |
935518 |
-7.71 |
|||
3 |
1.74 |
-1.42 |
930819 |
-7.17 |
|||
Mean |
1.73 |
-0.73 |
899477 |
-3.56 |
|||
0.0157 |
1 |
1.72 |
0.00 |
864940 |
0.42 |
||
2 |
1.75 |
-2.25 |
971827 |
-11.9 |
|||
3 |
1.79 |
-4.15 |
1072216 |
-23.5 |
|||
Mean |
1.75 |
-2.13 |
969661 |
-11.6 |
|||
Control |
1 |
1.70 |
819119 |
||||
2 |
1.70 |
819582 |
|||||
3 |
1.76 |
997657 |
|||||
4 |
1.68 |
776872 |
|||||
5 |
1.71 |
838618 |
|||||
6 |
1.75 |
959613 |
|||||
Mean |
1.72 |
868577 |
Section-by-Section and Average
Specific Growth Rates of the Control Group
(0
– 72 h)
Replicate No. |
Specific growth rate [d-1] |
Mean (0 - 72 h) |
SD ± |
CV |
Mean CV [%] |
|||
section-by-section |
||||||||
0 - 24 h |
24 - 48 h |
48 - 72 h |
||||||
Control |
1 |
1.53 |
2.06 |
1.49 |
1.70 |
0.317 |
18.7 |
13.4 |
2 |
1.65 |
1.94 |
1.51 |
1.70 |
0.220 |
13.0 |
||
3 |
1.70 |
2.00 |
1.59 |
1.76 |
0.212 |
12.0 |
||
4 |
1.58 |
1.94 |
1.52 |
1.68 |
0.228 |
13.6 |
||
5 |
1.79 |
1.79 |
1.53 |
1.71 |
0.148 |
8.67 |
||
6 |
1.55 |
2.04 |
1.66 |
1.75 |
0.254 |
14.5 |
||
Mean |
1.72 |
|
||||||
SD ± |
0.03 |
|
||||||
CV [%] |
1.92 |
|
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study p-Nitrobenzoylaminobenzamid trocken was found not to inhibit the growth of the freshwater green alga Desmodesmus subspicatus after 72 h. The EC50-values for inhibition of specific growth rate (ErC50) and yield (EyC50) after 72 h were > 0.304 mg/L, respectively. All effect levels are given based on initial measured concentrations of p-Nitrobenzoylaminobenzamid trocken.
- Executive summary:
The toxicity of p-Nitrobenzoylaminobenzamid trocken to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201.
The study was conducted under static conditions with an initial cell density of approximately 2 – 5 x 103cells/mL. The saturated solution and further four dilution levels were tested in a geometrical series with a dilution factor of 2, nominal: 6.25 - 12.5 - 25 - 50 - 100 % of the saturated solution corresponding to initial measured test item concentrations of 0.0157 – 0.0314 – 0.0712 – 0.153 – 0.304 mg/L. Three replicates were tested for each test item concentration and six replicates for the control. Environmental conditions were determined to be within the acceptable limits.
The concentrations of p-Nitrobenzoylaminobenzamid trocken were analysed at all concentration levels after 0 and 72 h via HPLC analysis. The measured concentrations of p-Nitrobenzoylaminobenzamid trocken at test end were in the range of 99 – 120 % of the initial measured values. The concentration of the lowest dilution level was below the LOQ of 0.03 mg/L. The lowest concentration was calculated from the second lowest test item concentration with the dilution factor of 2. All effect values are given based on initial measured concentrations of the test item.
NOEC, LOEC and EC - values of p-Nitrobenzoylaminobenzamid trocken (0-72 h)
based on initial measured test item concentrations [mg/L]
Rate-related Inhibition
NOEC
0.304
LOEC
> 0.304
ErC10
> 0.304
ErC20
> 0.304
ErC50
> 0.304
Inhibition of Yield
NOEC
0.304
LOEC
> 0.304
EyC10
> 0.304
EyC20
> 0.304
EyC50
> 0.304
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