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EC number: 202-863-0 | CAS number: 100-54-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study out of Japan.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Nicotinonitrile
- EC Number:
- 202-863-0
- EC Name:
- Nicotinonitrile
- Cas Number:
- 100-54-9
- Molecular formula:
- C6H4N2
- IUPAC Name:
- pyridine-3-carbonitrile
- Reference substance name:
- Pyridine-3-Carbonitrile
- IUPAC Name:
- Pyridine-3-Carbonitrile
- Details on test material:
- - Name of test material (as cited in study report): 3-Cyanopyridine
- Molecular formula (if other than submission substance):
- Molecular weight (if other than submission substance): 104.12
- Smiles notation (if other than submission substance):
- InChl (if other than submission substance):
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: white solid
- Physical state: solid
- Analytical purity: 99.9%
- Impurities (identity and concentrations):
- Composition of test material, percentage of components:
- Isomers composition:
- Purity test date:
- Lot/batch No.: 709S4067
- Expiration date of the lot/batch:
- Radiochemical purity (if radiolabelling):
- Specific activity (if radiolabelling):
- Locations of the label (if radiolabelling):
- Expiration date of radiochemical substance (if radiolabelling):
- Stability under test conditions: stable under normal handling conditions and during period of test
- Storage condition of test material:
- Other: soluble in water
Constituent 1
Constituent 2
Method
- Target gene:
- histidine
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- Obtained from Professor B.N. Ames of the University of California on May 27, 1983. Each of the bacteria strains was kept in frozen storage in an ultra-low temperature cessel at -80 degrees C or lower.
- Species / strain / cell type:
- E. coli WP2 uvr A
- Details on mammalian cell type (if applicable):
- Obtained from Professor Matsushima of the Medical Research Institute of Tokyo University on October 10, 1985. Each of the bacteria strains was kept in frozen storage in an ultra-low temperature cessel at -80 degrees C or lower.
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 fraction from liver of induced rats.
- Test concentrations with justification for top dose:
- 313, 625, 1250, 2500, 5000 micromole/plate (common ratio of 2). Maximum concentration of 50 mg/mL of 3-cyanopyridine in injection-use water, the solution was diluted with the same solvent at a common ration of 2 for use.
- Vehicle / solvent:
- Water
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- Positive controls:
- yes
- Positive control substance:
- other: acrylamide
- Remarks:
- TA-98 and TA-100 without S9
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- TA-1535 without S9
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- Positive controls:
- yes
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- Remarks:
- WP2 uvr A without S9
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- TA-1537 without S9
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene
- Remarks:
- All strains with S9
- Details on test system and experimental conditions:
- The test was conducted in duplicate by the preincubation method. Into a test tube was poured 0.1 mL of test substance solution, 0.5 mL of 0.1 M sodium phosphate buffer solution (pH 7.4) and 0.1 mL of bacterial suspension were added, and the mixture was shaken for 20 min at 37 degrees C. When S9 was present, 0.5 mL of S9 mix was added instead of the 0.1 M sodium phosphate buffer solution. Following preincubation, 2 mL of top agar was admixed to the test tube and the contents tereof were layered on a minimum glucose agar plate medium. Once the layered top agar had solidified, the bacteria were cultured for 48 hours at 37 degrees C. The development of bacterial colonies was observed with a steromicroscope. After checking for the presence of antibackterial properties due to the test substance, the number of reverse mutation colonies on the plate was counted with an automatic colony counter. In the reliminaty tests, one plate was employed at each concentration. In the main test, three plates were employed at each concentration and the test was conducted twice to confirm reproducibility. Further, a negative control substance (solvent) and a positive control substance for each bacteria strain were employed in place of the test substance solution to provide control groups that were handled in the same manner as the test substance groups.
- Evaluation criteria:
- For each of the test bacteria strains, irrespective of the presence or absence of S9 mix, when the number of reverse mutation colonies (average value) accompanying an increase in test substance concentration increased to twice or more that of the negative control value, and that increase was found to be reproducible, the test substance was judged to be positive. All other cases were judged to be negative.
- Statistics:
- Statistical menthods were not employed to judge the test results.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- In the results of two sets of main test conducted within the above-stated concentration range, no increase in the number of reverse mutation colonies of twice or more the level of the negative (solvent) control value was observed for any of the bacteria strains, irrespective of the presence or absence of S9 mix. Nor was any antibacterial property observed for any of the bacteria strains, irrespective of the presence or absence of S9 mix. Based on these results, it was concluded that the mutagenicity of 3-Cyanopyridine was negative.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
3-Cyanopyridine was tested in a standard Ames assay and no mutations were found with and without metabolic activation.
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