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EC number: 439-270-3 | CAS number: 260408-02-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 February - 20 March 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study has been performed according to OECD and EC guidelines and according to GLP principles.
- Qualifier:
- according to guideline
- Guideline:
- ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
- Version / remarks:
- (1989)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- (1992)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- (1984)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 and 100 mg/L (with and without algae)
- Sampling method
Frequency: at t=0 h, t=24 h and t=72 h
Volume: 10 ml
- Sample storage conditions before analysis: stored in a freezer (stability of samples during storage not confirmed) - Vehicle:
- no
- Details on test solutions:
- - Method:
Preparation started with a supersaturated solution at nominally 100 mg/l (not specified if prepared in test medium). This supersaturated solution was stirred for four days to ensure reaching maximum solubility. Then, the solution was filtered through a paper filter (Schleicher and Schuell 604) to remove the larger undissolved test substance particles (ca. > 5 µm). The lower concentrations were prepared by subsequent dilutions of the filtrate in test medium.
- Control: With test medium only
- Evidence of undissolved material (e.g. precipitate, surface film, etc): No - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: CCAP 278/4
- Source: In-house culture
- Pre-culture: 4 days before the start of the test, cells from the algal stock culture were inoculated in culture medium and maintained under the same conditions as used in the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- 24 mg CaCO3/L
- Test temperature:
- 22-23 °C
- pH:
- 0 (control): 8.1 at t=0 h and 10 at t=72 h
100 mg/L (100% filtrate): 7.9 at t=0 h and 9.8 at t=72 h
The pH increased by more than 1.5 unit, but this was related to a relative high rate of algal growth. Moreover, the cell density in controls increased normally (average factor >16 within 3 days). Hence, this deviation did not affect the validity criterion for algal growth. - Nominal and measured concentrations:
- - Nominal: 0 (control); 0.1; 1; 10 and 100 % of a filtrate prepared at 100 mg/L
- Measured in 100 mg/L (100% filtrate) test concentration
At 0 h (with /without algae):
Component 1 – 0.095/0.128 mg/L
Component 2 - <0.06/0.096 mg/L
Component 3 -0.675/<0.19 mg/L
At 24 h (with /without algae):
Component 1 – 0.048/0.027 mg/L
Component 2 - <0.06/<0.06 mg/L
Component 3 - <0.19/<0.19 mg/L
At 72 h (with /without algae):
Component 1 – 0.049/0.035 mg/L
Component 2 - <0.06/<0.06 mg/L
Component 3 - <0.19/<0.19 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml, all-glass
- Material, size, headspace, fill volume: All-glass , with 100 ml capacity filled with 50 ml of test solution
- Aeration: None
- Initial cells density: 1.0x10^4 cells/ml
- No. of vessels per concentration (replicates): 3, except for the highest concnetration where 6 were used
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes, M2-medium was used
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water purified by reverse osmosis and then passed over activated carbon and ion-exchange cartridges.
- Culture medium different from test medium: No
- Intervals of water quality measurement
pH: at the beginning and at the end of the test
Temperature: Every day
OTHER TEST CONDITIONS
- Photoperiod: Continuously
- Light intensity and quality: 96-123 µE.m^-2.s^-1, using TLD-lamps of the type "Cool-white" of 30 Watt
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: At the beginning of the test a counting chamber was used. Thereafter cell concentrations were measured spectrophotometrically
TEST CONCENTRATIONS
- Spacing factor for test concentrations: x10 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxicity up to solubility limit
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxicity up to solubility limit
- Details on results:
- - No inhibition of cell growth or reduction of growth rate was recorded at a 5 µm filtered solution prepared at a nominal test substance loading of 100 mg/L.
- Results with reference substance (positive control):
- - Potassium dichromate 72 h ErC50 was 1.1 mg/L (95% confidence interval 0.71-1.7 mg/L). This value was slightly higher that the upper limit of ISO standard range (1.03 mg/L). However, the lower limit of the 95% confidence interval was still within the ISO standard range.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this present test, the test substance did not inhibited cell growth or reduced growth rate was observed at a 5 µM filtered solution prepared at a nominal loading of 100 mg SPS-100/L. Therefore, it was concluded that no toxicity was observed up to solubility limit.
- Executive summary:
In a 72 h acute toxicity study, freshwater algae (Selenastrum capricornutum) were exposed to SPS-100 at: 0 (control); 0.1; 1; 10 and 100% of a filtrate prepared at a nominal loading rate of 100 mg/L.
The EC50 for both algal growth inhibition and growth rate reduction exceeded the maximum water solubility of the test substance at a 5 µm filtered test solution prepared at a nominal loading of 100 mg/L. Hence, as a consequence of the extremely low solubility of the test substance in water, concentration levels that might be toxic for algae could not be reached. Therefore, it was concluded that no toxicity was observed up to solubility limit.
The present toxicity study is classified as reliable without restrictions according to the OECD guideline Nr. 201.
Reference
Description of key information
A 72-h toxicity study freshwater algae (Selenastrum capricornutum) were exposed to SPS-100. The EC50 for both algal growth inhibition and growth rate reduction exceeded the maximum solubility of the test substance. Therefore, it was concluded that the test substance it is not acutely toxic up to the solubility limit. The present toxicity study is classified as reliable without restrictions according to the OECD guideline No. 201.
Key value for chemical safety assessment
Additional information
In a 72 h toxicity study, freshwater algae (Selenastrum capricornutum) were exposed to SPS-100 at: 0 (control); 0.1; 1; 10 and 100% of a filtrate (5 µm) prepared at a nominal loading rate of 100 mg/L. The EC50 for both algal growth inhibition and growth rate reduction exceeded the maximum solubility of the substance. Hence, as a consequence of the extremely low solubility of the substance, concentration levels that might be toxic for algae could not be reached. Therefore, it was concluded that the test substance it is not acutely toxic up to the solubility limit. The present toxicity study is classified as reliable without restrictions according to the OECD guideline No. 201.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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