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EC number: 421-750-9 | CAS number: 57280-22-5 TRIOXABICYCLOOCTAN; TRIOXABICYCLOOCTANE
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- effects on growth of green algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Apr 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- 92/69/EEC
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 1993
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: For the stock solution 127.5 mg of the test substance were dissolved in 1 L demineralized water. This solution was used for the highest test concentration of 100 mg/L (nominal concentration). To achieve the other test concentrations the stock solution was diluted.
- Controls: yes, blank control
- Test concentration separation factor: 10 - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Source: Sammlung von Algenkulturen, University Göttingen
- Age of inoculum (at test initiation): pre-culture taken 72 hours before test initiation from an stock culture up to 7 days old.
- Method of cultivation: the stock culture was dissolved in a liquid nutrient solution and subsequently transferred approximately once weekly.
ACCLIMATION
- Acclimation period: The pre-culture was incubated for 72 hours in an incubator shaker apparatus at approximately 23 °C. 'The same culturing media was used for pre-culture and test inoculum (nutrient solution diluted with demineralized water, stirred under aeration for ca. 1 hour and filtered under sterile conditions).
- Culturing media and conditions: different nutrient media used for stock culture and pre-culture/test inoculum. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 20.4 to 23.4 °C
- pH:
- 8.0 to 8.2
- Nominal and measured concentrations:
- 0.1, 1, 10, 100 mg/L (nominal test concentration)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: incubator shaker
- Material, size, headspace, fill volume: 100 mL
- Initial cells density: 10000 cells/mL
- Control end cells density: 924000 cells/mL
- No. of vessels per concentration (replicates): 3 replicates
- No. of vessels per control (replicates): 6 replicates
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: yes
- Intervals of water quality measurement: pH-value and temperature were measured at 0 and 72 hours of exposure.
OTHER TEST CONDITIONS
- Sterile test conditions: nutrient solution was filtered under sterile conditions before use
- Photoperiod: continuous light
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: 'Bürker'-counting chamber, electronic particle counting apparatus 'Coulter Counter', observation interval 24 hours.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Test concentrations: 0.1, 1, 10, 100 mg/L (nominal concentration) - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Any stimulation of growth found in any treatment: no - Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results incl. tables'.
- Conclusions:
- The test substance had no inhibitory effect on the growth of Desmodesmus subspicatus (Scenedesmus subspicatus).
- Executive summary:
A study was performed to determine the effects of the test substance on the growth of the green freshwater algae Desmodesmus subspicatus (Scenedesmus subspicatus). The study was conducted in accordance with the OECD Guideline for Testing of Chemicals No 201 'Alga, Growth Inhibition Test' and the EC guideline, EC Directive 92/69/EEC, Annex C3, the 'Algae inhibition test'. The test substance was incubated in an aqueous solution including nutrients for test duration of 72 hours. The test concentrations were 0, 0.1, 1, 10, and 100 mg/L (nominal concentrations). The cell densities were measured at 24 hour intervals. The calculated biomass and growth rate of each concentration were compared to those of the controls and the inhibition was calculated. According to the measurements, no growth inhibition was observed, and the test substance had no inhibitory effect on algae growth at test substance concentrations up to 100 mg/L (nominal concentration).
Reference
Validity criteria for the measurement of the algae toxicity
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
The biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. This corresponds to a specific growth rate of 0.92 day-1. This criterion may not be met when species that grow slower than those listed in Annex 2 of OECD TG 201 are used. In this case, the test period should be extended to obtain at least a 16-fold growth in control cultures, while the growth has to be exponential throughout the test period. The test period may be shortened to at least 48 hours to maintain unlimited, exponential growth during the test as long as the minimum multiplication factor of 16 is reached. | During the test, the biomass in the control increased exponentially by a factor >16 and the specific growth rate was 1.51 day-1 within the 72-hour test period. | Yes |
The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3, for 72-hour tests) in the control cultures must not exceed 35%. This criterion applies to the mean value of coefficients of variation calculated for replicate control cultures. | The mean coefficent of variation for section-by-section specific growth rates in the control was <6% | yes |
The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures must not exceed 7% in tests with Pseudokirchneriella subcapitata and Desmodesmus subspicatus. For other less frequently tested species, the value should not exceed 10%. | The coefficent of variation of average specific growth rates (0-72h) in the control was <1% | Yes |
The cultures should be maintained at a temperature in the range of 21 to 24°C, controlled at ± 2°C. | During the test, cultures were maintained at a temperature range of 20.4 to 23.4°C. The temperature un the incubator chamber were slightly below the recommended range. However, as temperature were only 0.6°C below the recommended range and di not cary by mor thean +/- 2°C this deciation should not have had any influence on the test results. | Yes |
Description of key information
A study was performed to determine the effects of the test substance on the growth of the green freshwater algae Desmodesmus subspicatus (Scenedesmus subspicatus). The study was conducted in accordance with the OECD Guideline for Testing of Chemicals No 201 'Alga, Growth Inhibition Test' and the EC guideline, EC Directive 92/69/EEC, Annex C3, the 'Algae inhibition test'. The test substance was incubated in an aqueous solution including nutrients for test duration of 72 hours. The test concentrations were 0, 0.1, 1, 10, and 100 mg/L (nominal concentrations). The cell densities were measured at 24 hour intervals. The calculated biomass and growth rate of each concentration were compared to those of the controls and the inhibition was calculated. According to the measurements, no growth inhibition was observed, and the test substance had no inhibitory effect on algae growth at test substance concentrations up to 100 mg/L (nominal concentration).
Key value for chemical safety assessment
Additional information
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