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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August - November 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- yes
- Remarks:
- The pH of the medium was not adjusted to the range stated in the Study Plan. The pH of all test vessels was within the range stated by the validity criterion at the end of the test. This deviation did not impact the validity or outcome of the study.
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- - Source of test organisms: Activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Preperation of inoculum for exposure: The freshly obtained sludge was kept under continuous aeration until further treatment. Before use, the sludge was coarsely sieved (1 mm) and washed with mineral medium. After treatment, the concentration of suspended solids (SS) was determined to be 3 g/L in the concentrated sludge as used for the test. The sludge was used as inoculum at a concentration of 2 mL per litre of mineral medium, leading to a final concentration SS of 5 mg/L. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 33 mg/L
- Based on:
- ThOD
- Remarks:
- 80 mg O2/L
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of the medium: mineral medium as recommended in the OECD Guideline
- Test temperature: 20-22 °C
- pH: 7.7 at test start
- pH adjusted: no
- Light conditions: The test media were excluded from light, brown colored glass bottles were used.
TEST SYSTEM:
- Culturing apparatus: Lovibond BD600-GLP manometric respirometry system equipped with an inductive stirring system.
- Number of flasks: 2 flasks containing test item and inoculum
SAMPLING:
- Sampling was done on day 0-4-7-11-14-18-21-25-28
CONTROL AND BLANK SYSTEM
- 2 flasks containing only inoculum
- 1 flask containing reference item and inoculum
- 1 flask containing inoculum, and test item and reference item in amounts contributing equally to the total ThOD - Reference substance:
- acetic acid, sodium salt
- Key result
- Parameter:
- other: ThOD
- Value:
- 2.38
- Remarks on result:
- other: mg O2/ mg test item
- Details on results:
- The relative biodegradation values calculated from the measurements performed during the test period revealed 5% and 34% biodegradation of test item based on ThOD, for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met.
- Results with reference substance:
- Sodium acetate (CAS 127-09-3): ThOD 0.78 mg O2/ mg reference substance
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Ths substance is not readily biodegradable.
- Executive summary:
The objective of the study was to evaluate the test item Soya/Linseed Oil Fatty Acid-BADGE reaction product for its ready biodegradability in an aerobic aqueous medium with microbial activity introduced by inoculation with activated sludge; Manometric Respirometry Test.
The study procedures described in this report were in compliance with the OECD guideline No. 301 F, 1992.
The test item was a clear colourless viscous liquid UVCB. The Theoretical Oxygen Demand (ThOD) of the test item was determined to be 2.38 mg O2/mg. The test item was tested in duplicate at a target concentration of 33 mg/L, corresponding to a ThOD of 80 mg O2/L. The ThOD was based on the results of elemental composition analysis.
The study consisted of six bottles:
· 2 inoculum blanks (no test item),
· 2 test bottles (test item),
· 1 procedure control (sodium acetate) and
· 1 toxicity control (test item plus sodium acetate).
In order to transfer test item to the test vessels, the test item was weighed on an inert carrier, which was transferred to the test bottles containing medium with microbial organisms and mineral components. The test solutions were continuously stirred during the test to ensure optimal contact between the test item and test organisms. Test duration was28 days.
The relative biodegradation values calculated from the measurements performed during the test period revealed 5% and 34% biodegradation of test item based on ThOD, for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met.
In the toxicity control, the test item was found not to inhibit microbial activity.
One of the criteria for acceptability of the test was not met. However, the Sponsor has indicated that the observed behaviour was consistent with the nature of the test item. Furthermore, both biodegradation values observed would lead to the conclusion that the test item is not readily biodegradable. Therefore, this study was considered to be valid and is reported with a >20% difference between replicate A and B.
In conclusion, Soya/Linseed Oil Fatty Acid-BADGE reaction product was designated as not readily biodegradable.
Reference
Acceptability of the Test:
1. The reference item was biodegraded by at least 60% (actual value: 88%) within 14 days.
2. The difference of duplicate values for %-degradation of the test item was higher than 20 (29% at the end of the test).
3. The total oxygen uptake in the blank at the end of the test did not exceed 30 mg O2/L (average actual value: 9 mg O2 per litre).
4. The pH value in the test item bottles at the end of the test was within the range 6-8.5 (7.8 and 7.7).
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
The objective of the study was to evaluate the test item Soya/Linseed Oil Fatty Acid-BADGE reaction product for its ready biodegradability in an aerobic aqueous medium with microbial activity introduced by inoculation with activated sludge; Manometric Respirometry Test.
The study procedures described in this report were in compliance with the OECD guideline No. 301 F, 1992.
The test item was a clear colourless viscous liquid UVCB. The Theoretical Oxygen Demand (ThOD) of the test item was determined to be 2.38 mg O2/mg. The test item was tested in duplicate at a target concentration of 33 mg/L, corresponding to a ThOD of 80 mg O2/L. The ThOD was based on the results of elemental composition analysis.
The study consisted of six bottles:
· 2 inoculum blanks (no test item),
· 2 test bottles (test item),
· 1 procedure control (sodium acetate) and
· 1 toxicity control (test item plus sodium acetate).
In order to transfer test item to the test vessels, the test item was weighed on an inert carrier, which was transferred to the test bottles containing medium with microbial organisms and mineral components. The test solutions were continuously stirred during the test to ensure optimal contact between the test item and test organisms. Test duration was28 days.
The relative biodegradation values calculated from the measurements performed during the test period revealed 5% and 34% biodegradation of test item based on ThOD, for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met.
In the toxicity control, the test item was found not to inhibit microbial activity.
One of the criteria for acceptability of the test was not met. However, the Sponsor has indicated that the observed behaviour was consistent with the nature of the test item. Furthermore, both biodegradation values observed would lead to the conclusion that the test item is not readily biodegradable. Therefore, this study was considered to be valid and is reported with a >20% difference between replicate A and B.
In conclusion,Soya/Linseed Oil Fatty Acid-BADGE reaction product was designated as not readily biodegradable.
Similar results were obtained with the source substance BADGE.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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