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EC number: 607-988-8 | CAS number: 2668-75-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Mar - Apr 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted 04 Apr 1984
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 17-Acetoxy-1,4,6-pregnatriene-3,20-dione
- EC Number:
- 607-988-8
- Cas Number:
- 2668-75-9
- Molecular formula:
- C23 H28 O4
- IUPAC Name:
- 17-Acetoxy-1,4,6-pregnatriene-3,20-dione
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: saturated solution and dilutions (1:2, 1:4, 1:8, 1:10)
- Sampling method: 0 and 48 h all vessels pooled
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: For the stock solution 100 mg ZK 5560 were suspended in 1000 ml tap water and constantly stirred for 24 hours. Thereafter, the suspension was filtered through a glassfibre filter. This solution was used as the highest test concentration. Aliquots of the solution were further diluted 1:2, 1:4, 1:8 and 1:10 with tap water in order to prepare the appropriate test concentrations.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): less 24 h
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20.0 - 21.6 °C
- pH:
- 8.0 - 8.8
- Dissolved oxygen:
- 6.4 - 9.4 mg/L
- Nominal and measured concentrations:
- Nominal: 100, 50, 25, 12.5, 10 mg/L
measured: 13.85, 6.8, 3.43, 1.62, 1.22 mg/L - Details on test conditions:
- TEST SYSTEM
20 Daphnia were used for each test solution and 20 for the dilution water control, randomly distributed into blocks of five. LabelIed 100 mL test vessels were used and filled with 45 mL of the test solution or tap water (control group) and 5 mL of the dilution water with the introduction of the Daphnia. The test was performed with 4 repllicates of each test solution and the dilution water control with 5 Daphnia in each vessel. One additional vessel (measurement vessel) without Daphnia for each test and control solution, respectively, was used for the measurements of pH, temperature and oxygen content. The Daphnia were exposed to the test solutions and tap water (control) for a period of 48 hours under static conditions. The light/dark rhythm was adjusted to 12 hours/12 hours. - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10.7 mg/L
- 95% CI:
- >= 8.8 - <= 14
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Any other information on results incl. tables
Table 1: Cumulative immobilization (%) of Daphnia magna (n = 20)
Measured concentraton of Delta-1,6 -Hydroxyprogesteronacetat (mg/L) | 24 hours | 48 hours |
0 Control (tap water) | 5 | 5 |
1,2 | 0 | 0 |
1,6 | 5 | 15 |
3,4 | 0 | 10 |
6,8 | 5 | 15 |
13,9 | 15 | 75 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50/48 hours of the test substance to Daphnia magna was calculated to be 10.7 mg/L [95% confidence limits (8.8 - 14.0 mg/L)] on the basis of measured concentrations. The highest concentration with no effects was 1.2 mg/L.
- Executive summary:
20 Daphnia were used for each test solution of Delta-1,6 -Hydroxyprogesteronacetat and 20 for the dilution water control (tap water). The Daphnia were exposed for a period of 48 hours under static conditions. Immobilization was recorded at 24 hours and 48 hours. For the preparation of the test solutions, 100 mg/L of the test substance were suspended in tap water and stirred for 24 hours. This suspension was filtered through a glassfibre filter. The saturated solution obtained by this procedure served as the highest concentration. It was further diluted 1:2, 1:4, 1:8 and 1:10. The test temperature was in the range of 20.0 to 21.6 °C, the pH ranged between 8.0 and 8.8, and the oxygen concentration between 6.4 and 9.4 mg/L. The light/dark rhythm was adjusted to 12 hours/12 hours. Samples for the concentration analysis by HPLC in combination with UV detection were taken at the beginning and 48 hours after start of exposure. The analytical results gave concentrations of 13.9 mg/L (saturated solution), 6.8 mg/L (1:2 dilution), 3.4 mg/L (1:4 dilution), 1.6 mg/L (1:8 dilution) and 1.2 mg/L (1:10 dilution). Immobilized Daphnia were observed in all test solutions except in the lowest concentration. One immobilized Daphnia was observed in the control solution. The EC50/48 hours to Daphnia magna was calculated to be 10.7 mg/L [95% confidence limits (8.8 - 14.0)] on the basis of measured concentrations. The highest concentration with no effects was 1.2 mg/L.
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