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Diss Factsheets
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EC number: 401-610-3 | CAS number: 122012-52-6 GENIPLEX A
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April and May 1986.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- EEC directive 84/449/EEC
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Range finding test
A range finding test preceded the definitive assay. Five concentrations of the test item were prepared from 0.1 mg/L to 1000 mg/l.
Definitive Assay
For the second assay, 5 concentrations of the test item were prepared as follows: 0.65 mg/L, 0.313 mg/L, 0.156 mg/L, 0.078 mg/L and 0.039 mg/L. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Daphnids were obtained from a continuous laboratory culture of Daphnia magna.
ACCLIMATION:
- Acclimation period: The cultivation of the daphnia is performed in a way that the animals are transferred in new test medium at an interval 24 h prior to test
- Type and amount of food: Chlorella sorokiniana
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
- Daphnias used for the test are not older than 24 hr. Therefore, in every case, daphnia are transferred into fresh medium one day before starting of the daphnia test in order to get daphnia for the test younger than 24 hr.
- 24 h prior to the test, egg bearing females were transferred by pipette to fresh EPA soft water. Emerged, 1st instar daphnids (6-24 h old), produced by diploid parthenogenesis, were removed for the test.
- Type and amount of food: Chlorella sorokiniana - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20 ± 1°C
- pH:
- 6.35 - 7.08 during definitive main test (assay 1: 6.59-7.08; assay 2 6.35-6.94 at zerotime)
assay 2: 7.06-7.47 at 48 h - Dissolved oxygen:
- 81-99% during definitive main test (assay 1: 89-99%; assay 2 81-87% at zerotime)
assay 2: 70-79% at 48 h - Nominal and measured concentrations:
- Nominal: 0, 0.039, 0.078, 0.156, 0.313, 0.625 mg/L
Found (t48h): 0.097, 0.069, 0.095, 0.095, 0.138 , 0.141mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Material, size, headspace, fill volume: 450mL, glass
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of daphnia per replicate: 10
- Aeration: static
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted EPA soft water was used. Water was vigorously aerated for 3 hrs prior to the
test but not during the test.
TEST CONCENTRATIONS
- Range finding study:
Five concentrations of the test item were prepared from 0.1 mg/L to 1000 mg/L. Aliquots of each solution (250 mL) were placed in duplicate 400 mL capacity glass beakers and 5 daphnids transferred to each.
- Test concentrations: 0.625, 0.313, 0.156, 0.078, 0.039 and 0 mg/L
OTHER TEST CONDITIONS
- Photoperiod: 16 hr of light and 8 hr of darkness
- pH and oxygen concentrations were determined in each test vessel during the definitive assays, at zero time, and after 48 h for the second assay only - Reference substance (positive control):
- not specified
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.195 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.058 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - In water, the test item is rapidly hydrolysed leading to the formation of zinc salts (predominantly zinc oxide) with the quantitative release of ammonia. Attempts were made to measure ammonia-N by a colorimetric assay, however concentrations were generally too low to derive reliable results. -
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Limit test: no
- Dose-response test: yes - Validity criteria fulfilled:
- yes
- Conclusions:
- The study was performed according to OECD TG 202 without deviations, the results were so obtained via a scientifically reasonable method. Hence, there is no doubt that the obtained results are not reliable:
The following EC-values were determined accordingly after 48 hours:
Mobility:
after 24 h: EC50 = 0.195 mg/L
after 48 h: EC50= 0.058 mg/l
Based on these results, the test item needs to be classified as aquatic toxic 1 (acute and chronic) to the aquatic environment. - Executive summary:
The 48-hr-acute toxicity of the test item to daphnia magna was studied under static conditions. Test species were exposed to control and test chemical at nominal concentration of 0.625, 0.313, 0.156, 0.078, 0.039 and 0 mg/L for 48-hr. Immobilization and sublethal effects were observed test termination. The 48 – hour EC50 was 0.058 mg/L with a 95% confidence interval of 0.047-0.072 mg/L.
Based on these results, the test item needs to be classified as aquatic toxic 1 (acute and chronic) to the aquatic environment.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test Organism Age (e.g. 1stinstar): less than 24 h
Test Type (Static):
EC50: 0.058 mg/L 95% C.I.: 0.047-0.072 mg/L.
Reference
EC50 values from statistical analysis using the Weil method
Time interval | EC50 (mg/L) | 95% confidence intervals |
24 h |
0.195 | 0.128-0.297 |
48 h | 0.058 | 0.047-0.072 |
Description of key information
Acute toxicity to aquatic invertebrates: EC50 (48h) = 0.058 mg/L, based on immobilisation, for Daphnia magna (OECD 202, GLP, static)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.058 mg/L
Additional information
Based on these results, the test item needs to be classified as aquatic toxic 1 (acute and chronic) to the aquatic environment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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