Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 228-602-0 | CAS number: 6303-30-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- Type : mixed population of aquatic microorganisms
Origin : secondary effluent of a wastewater treatment plant treating predominantly domestic sewage (Wupper area water authority, WWTP Odenthal)
Date of collection : 2020-10-05
Pre-treatment : separation of coarse particles by filtration, aeration of the resulting inoculum for one day
Effluent concentration of reaction mixture : 5 mL/L (5.8 mL/1.16 L) - Duration of test (contact time):
- 28 d
- Initial conc.:
- 5 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- Pre-treatment of the test item
A stock solution was prepared to give the desired test concentration. 1 g were added to 1 litre of mineral medium and stirred for 24 hours on a magnetic stirrer. 5.8 mL of the stock solution were given to the test flasks, filled with ca. 300 mL of mineral medium. Afterwards the inoculum was added and the flasks volume was made up to 1.16 L with mineral medium and the flasks were closed free from air bubbles with glass stoppers.
Concentration of stock solution : 1 g/L
pH value of the stock solution : 7.5
Initial concentration test item : 5 mg/L (5.8 mg/1.16 L)
Pre-treatment of the reference compound
Reference compound name : sodium benzoate (Acros Organics)
Coding number : Ökotox_7/04
Purity : 99.6 %
Batch-no. : A0412860
Expiry date : 2022-10-31
A stock solution was prepared to give the desired test concentration. 500mg were added to 0.5 litres of deionised water and stirred for one hour on a magnetic stirrer. 3.4 mL of the stock solution were given to the test flasks, filled with ca. 300 mL of mineral medium. Afterwards the inoculum was added and the flasks volume was made up to 1.16 L with mineral medium and the flasks were closed free from air bubbles with glass stoppers.
Concentration of stock solution : 1 g/L
pH value of the stock solution : 7.4
Initial concentration reference compound : 2.9 mg/L (3.4 mg/1.16 L)
Pre-treatment of the toxicity control
Two stock solutions were prepared to give the desired test concentrations. 5.8 mL of the test item stock solution and 3.4 mL of the reference compound stock solution were given to the test flasks, filled with ca. 300 mL of mineral medium. Afterwards the inoculum was added and the flasks volume was made up to 1.16 L with mineral medium and the flasks were closed free from air bubbles with glass stoppers.
Initial concentration test item : 5 mg/L (5.8 mg/1.16 L)
Initial concentration reference compound : 2.9 mg/L (3.4 mg/1.16 L)
Exposure conditions
Test volume : 1.16 L
Incubation time : 28 days
Incubation temperature : 22 2 °C - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- The following flasks were used:
Test suspension (18 flasks)
A measured volume of mineral medium containing a known concentration of the test item (as the nominal sole source of organic carbon) and inoculum.
Reference control (10 flasks)
In order to check the procedure, the reference compound sodium benzoate was tested in parallel to the normal test runs.
Inoculum blank (18 flasks)
A measured volume of mineral medium containing only inoculum.
Toxicity control (10 flasks)
Toxic effects of the test item were determined in toxicity control flasks containing test item, reference compound and inoculum.
Degradation was followed by analysis of dissolved oxygen over a 28-day period. The amount of oxygen taken up by the microbial population during biodegradation of the test item, corrected for uptake by the blank inoculum run in parallel, was expressed as a percentage of ThOD.
The test lasted for 28 days. - Parameter:
- % degradation (DOC removal)
- Value:
- 0
- Sampling time:
- 7 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 1
- Sampling time:
- 14 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 0
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 0
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Remarks:
- The reference compound sodium benzoate showed 79 % degradation after 14 days.
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- According to OECD 301 D Diisobutyl phosphate is considered to be not readily biodegradable.
- Executive summary:
The study was performed to assess the ready biodegradability of Diisobutyl phosphate. The study was conducted in accordance with the Council Regulation (EC) 440/2008 Method C.4-E "Closed Bottle Test" (2008). This test method is in all essential parts identical with OECD Guideline 301 D (adopted July 1992).
The test item Diisobutyl phosphate in a mineral medium was inoculated with secondary effluent to result in completely filled, closed flasks and incubated under aerobic conditions for 28 days. During this period, the biodegradation was followed by analysis of dissolved oxygen. The amount of oxygen taken up by microorganisms which reduced the test chemical, corrected for uptake by the blank inoculum run in parallel, was expressed as a percentage of theoretical oxygen demand (ThOD).
Diisobutyl phosphate showed:
0 % degradation after 7 days
1 % degradation after 14 days
0 % degradation after 21 days
0 % degradation after 28 days
The biodegradation of Diisobutyl phosphate was 0 % (ThOD) after 28 days. The test item failed the pass level requested in test method OECD 301 D which is a biodegradation ≥ 70 % DOC or ≥ 60 % ThOD.
According to OECD 301 D the test item is therefore considered to be not readily biodegradable.
Reference
Degradation of the test item
Test concentration : 5 mg/L
Theoretical oxygen demand : 1.827 mg O2/mg
Test item | DO depletion after n days [mg/L] | |||||||
3 d | 7 d | 10 d | 14 d | 17 d | 21 d | 24 d | 28 d | |
No.1: (mt0 - mtx) - (mb0 - mbx) | 0.0 | 0.0 | 0.0 | 0.1 | 0.0 | 0.0 | 0.0 | 0.0 |
No.2: (mt0 - mtx) - (mb0 - mbx) | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
D1: % degradation | 0% | 0% | 0% | 1% | 0% | 1% | 0% | 0% |
D2: % degradation | 0% | 0% | 0% | 0% | 0% | 0% | 0% | 1% |
% degradation, mean | 0% | 0% | 0% | 1% | 0% | 0% | 0% | 0% |
Comments: Abbreviations according to test guideline
Degradation of the reference compound
Test concentration : 2.9 mg/L
Theoretical oxygen demand : 1.665 mg O2/mg
Reference compound | DO depletion after n days [mg/L] | |||||||
3 d | 7 d | 10 d | 14 d | 17 d | 21 d | 24 d | 28 d | |
No.1: (mt0 - mtx) - (mb0 - mbx) | 3.7 | 3.8 | 3.8 | 4.0 | ||||
No.2: (mt0 - mtx) - (mb0 - mbx) | 3.7 | 3.8 | 3.8 | 4.1 | ||||
D1: % degradation | 76% | 79% | 78% | 82% | ||||
D2: % degradation | 76% | 79% | 78% | 84% | ||||
% degradation, mean | 76% | 79% | 78% | 83% |
Comments: none
Degradation of the toxicity control
Test concentration test item : 5 mg/L
Test concentration reference compound : 2.9 mg/L
Toxicity control | DO depletion after n days [mg/L] | |||||||
3 d | 7 d | 10 d | 14 d | 17 d | 21 d | 24 d | 28 d | |
No.1: (mt0 - mtx) - (mb0 - mbx) | 3.7 | 3.9 | 3.9 | 4.0 | ||||
No.2: (mt0 - mtx) - (mb0 - mbx) | 3.5 | 3.8 | 3.9 | 4.0 | ||||
D1: % degradation | 26% | 28% | 28% | 28% | ||||
D2: % degradation | 25% | 27% | 28% | 28% | ||||
% degradation, mean | 25% | 28% | 28% | 28% |
Comments: The used concentration of the test item is not toxic to bacteria.
Description of key information
The biodegradation of Diisobutyl phosphate was 0 % (ThOD) after 28 days. The test item failed the pass level requested in test method OECD 301 D. According to OECD 301 D the test item is considered to be not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.