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EC number: 451-330-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 6 January 2004 to 22 January 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- (2E,5Z)-5,6,7-trimethylocta-2,5-dien-4-one
- Cas Number:
- 357650-26-1
- Molecular formula:
- C11H18O
- IUPAC Name:
- (2E,5Z)-5,6,7-trimethylocta-2,5-dien-4-one
- Reference substance name:
- (2E,5E)-5,6,7-trimethylocta-2,5-dien-4-one
- Cas Number:
- 847144-75-6
- Molecular formula:
- C11H18O
- IUPAC Name:
- (2E,5E)-5,6,7-trimethylocta-2,5-dien-4-one
- Test material form:
- liquid
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Aspect: Slightly yellow liquid
Purity: 96.7%
Expiry date: 12 September 2004
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 1.0, 1.8, 3.2, 5.6 and 10 mg/L
- Sampling method: 10 mL from the approximate centre of the test vessel in duplicate for all test concentration and bloak control.
- Sample storage conditions before analysis: samples were stored in a freezer until analysis
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Preparation of test solution started with a stock solution of 100 mg/L applying a short period of magnetic stirring to accelerate the dissolving of the test susbtance in the test medium. The lower test concentrations were prepared by subsequent dilutions of the stock in the test medium. The final test solution were all clear and colourless
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Species: Daphnia magna (Crustacea, Cladocera) STraus 1820
- Characteristics : Young Daphnia with an age of < 24 hours
Breeding -
Start of Each Batch : With new-born animals (less than 3 days old) by placing approximately 250 of them into 5 Litres of medium in all-glass culture vessel
Maximum age of Cultures : 4 weeks
Renewal of the Cultures : After 7 days of cultivation half of the medium twice a Week
Temperature of Medium : 18 - 22 °C
Feeding : Daily, a suspension of fresh-water algae
Medium : M7
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- In accordance with OECD TG 202 guideline
Test conditions
- Hardness:
- The hardness of the ISO medium is 250 mg/L expressed as CaCO3 and the pH is 8.0 +/- 0.2 after aeration
- Test temperature:
- The temperature of the test medium was 20.0°C at the start of the test. The temperature continuously measured in a temperature control vessel varied between 20.4°C and 21.0°C during the test, and complied with the requirements as laid down in the protocol.
- pH:
- Measured pH remained within the limits prescribed by the protocol (6.0 -8.5; not varying by more than 1 unit)
Measures: 8.1 and 8.0 - Dissolved oxygen:
- Measured oxygen remained within the limits prescribed by the protocol (>7 mg/l at the start, >= 5 mg/L at the end of the test)
Measures: 9.0 and 9.1 - Nominal and measured concentrations:
- Blank control; 1.0; 1.8; 3.2; 5.6; 10 mg/L (Nominal) / Blank control; 0.87; 1.44; 2.78; 4.58; 8.70 mg/L (Measured at T=0h) / Blank control; 0.75; 1.31; 2.63; 4.09; 7.71 (Measured at T=48h)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL all glass
- Material, size, headspace, fill volume: Glass
- Aeration: No aeration of the test solutions.
- No. of organisms per vessel: 5 per vessel containing 80 ml medium
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: No, ISO Medium M7, renewed by 50% twice per week.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours photoperiod daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobility (including mortality), 24 hours and at 48 hours.
VEHICLE CONTROL PERFORMED: yes/no
RANGE-FINDING STUDY
- Test concentrations: Blank control; 0.1; 1.0; 10; 100 mg/L (nominal)
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.1 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- The actual responses in this reference test with K2Cr2O7 are within the ranges of the expected responses at the different concentrations. Hence, the sensitiity of this batch of D. magna was in agreement with the historical data collected at NOTOX.
Any other information on results incl. tables
Table 2: Acute immobilisation of Daphnia after 24 hours and 48 hours in the final EC50 test
Concentration GR-85 -2517 Nominal / Initial (mg/L) |
Vessel number | Number of Daphnia exposed | Response at 24 h | Response at 48h | ||
Number | Total % | Number | Total % | |||
Blank control | A B C D |
5 5 5 5 |
0 0 0 0 |
0 | 0 0 0 0 |
0 |
1.0 / 0.87 | A B C D |
5 5 5 5 |
0 0 0 0 |
0 | 0 0 0 0 |
0 |
1.8 / 1.44 | A B C D |
5 5 5 5 |
0 0 0 0 |
0 | 0 0 0 0 |
0 |
3.2 / 2.78 | A B C D |
5 5 5 5 |
0 1 1 1 |
15 | 3 4 5 5 |
85 |
5.6 / 4.58 | A B C D |
5 5 5 5 |
5 5 5 5 |
100 |
5 5 5 5 |
100 |
10 / 8.70 |
A B C D |
5 5 5 5 |
5 5 5 5 |
100 |
5 5 5 5 |
100 |
Table 3: Effect parameters
Parameter | Concentration GR-85 -2517 Initial (mg/L) | 95% confidence interval |
Observed NOEC | 1.4 | |
24h-EC50 | 3.1 | 2.8 - 3.6 |
48h-EC50 | 2.2 | 2.0 - 2.5 |
Table: Acute immobilization of Daphnia after 24 and 48 hours in the reference test with potassium dichromate
Concentration (mg/L) | Number Exposed | % immobile | Expected response (%) after 48h* | ||
24h | 48 h | Minimal | Maximal | ||
Blank control | 10 | 0 | 0 | 0 | 10** |
0.10 | 10 | 0 | 0 | 0 | 10 |
0.18 | 10 | 0 | 0 | 0 | 10 |
0.32 | 10 | 0 | 0 | 0 | 30 |
0.56 | 10 | 0 | 0 | 0 | 100 |
1.0 | 10 | 0 | 90 | 40 | 100 |
1.8 | 10 | 100 | 100 | 100 | 100 |
*Based on historical data of previous years (n>60)
** A maximum response of 10% does not invalidate the result of the test
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study GR-85-2517did not induce acute immobilisation of Daphnia magna at an initial exposure concentration of 1.4 mg/L after 48 hours of exposure
The 48h-EC50 based on initial exposure concentrations was 2.2 mg/l with a 95% confidence interval between 2.0 and 2.5 mg/L - Executive summary:
The study procedures described in this report were based on ISO International Standard 6341, 1996. In addition, the procedures were designed to meet the test methods and validity criteria of the EEC directive 92769, Part C.2, 1992 and the OECD guideline No. 202 Part I, 1984.
The batch of GR-85 -2517 tested was a slightly yellow liquid with a purity of 96.7%. GR-85 -2517 was completely soluble in test medium at the concentration tested.
The project started with a range-finding test. Ten Daphnia per concentration (In duplicate, 5 per vessel) were exposed to a range of 0.1 to 100 mg/L increasing by a factor 10 and to a blank-control. After 24 hours of exposure all organism exposed to nominally 10 and 100 mg/L became immobilised. No immobilisation was observed at the lower test concentration. The 48h-EC50 appeared to be between nominal concentration of 1.0 and 10 mg/L. Analytical results showed that measured GR-85 -2517 concentration in samples taken taken from nominally 1.0 mg/L decreased from 0.85 mg/L at the start to 0.69 mg/L after 48 hours. Hence, GR-85 -2517 concentration did not decrease by more than 20% during the test.
The project was continued with a final EC50 test exposing twenty Daphnia per test group (in quadruplicate, 5 per vessel) to a blank control and GR-85 -2517 concentrations of 1.0, 1.8, 3.2, 5.6 and 10 mg/L. Samples for analytical confirmation of actual exposure concentrations were taken from all test solutions at the start and the end f the 48 hour test period.
Analysis of the samples taken at the start of the final test showed mean measured intitial concentrations of 0.87, 1.44, 2.78, 4.58 and 8.70 mg/L at the nominal concentration of 1.0, 1.8, 3.2, 5.6 and 10 mg/L respectively. During the exposure period the measured concentrations did not decrease by more than 20% of the initial concentrations.
The study met the acceptability criteria prescribed by the protocol and was considered valid.
GR-85 -2517 did not induce acute immobilisation of Daphnia magna at an initial exposure concentration of 1.4 mg/L after 48 hours of exposure (NOEC).
The 48h-EC50 based on initial exposure concentrations was 2.2 mg/L with a 95% confidence interval between 2.0 and 2.5 mg/L.
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