Boronic acid, B-(2-fluoro-3-methoxyphenyl)-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

31st May 2018 - 2nd July 2018

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: MEP, P.R. China, the Guideline for the Testing of Chemicals, Degradation and Accumulation (the Second Edition), No. 301F "Manometric Respirometry Test. (2013.9)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(2-fluoro-3-methoxyphenyl)boronic acid

EC Number:

609-099-0

Cas Number:

352303-67-4

Molecular formula:

FC6H3(OCH3)B(OH)2

IUPAC Name:

(2-fluoro-3-methoxyphenyl)boronic acid

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

Lot number: 18051400
Appearance: Off-white powder
Storage: Room temperature 15-25 C
Purity: 100.2%

Study design

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Details on inoculum:

A fresh sample of activated sludge was collected from a sewage treatment plant as recommended by the OECD Guidelines for the Testing of Chemicals. A fresh sample of activated sludge was collected from the aeration tank of Shenyang North Sewage Treatment Plant 4 days before the test exposure began. It is a well-functioning treatment plant receiving predominantly domestic sewage.

Duration of test (contact time):

28 d

Results and discussion

% Degradation

Key result

Parameter:

other: degradation percentage was obtained from BOD/ThOD as per guidelines described in OECD 301 F

Value:

14.3

Sampling time:

28 d

Remarks on result:

other:

Details on results:

Based on the results, the percentage biodegradation within 28 days did not exceed the pass level of 60% ThOD. The test item did not meet the criteria for ready biodegradability under the described test conditions.

BOD5 / COD results

Results with reference substance:

The biodegradation of the reference substance (Sodium benzoate) attained 64.2% after Day 5, 95.3% after Day 14 and 93.6% after Day 28

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

All validity criteria were fulfilled as per described test guidelines.

Interpretation of results:

not readily biodegradable

Conclusions:

The test item did not meet the criteria for ready biodegradability under the described test conditions.

Executive summary:

The ready biodegradability test of FMPBA was conducted with unacclimatised sewage micro-organisms by measuring oxygen consumption over the 28-day test period.

The biodegradation of test item after 28 days averaged 14.3% (2 replicates). The test item did not exhibit a percentage biodegradation exceeding the pass level of 60% within the 28-day period.

The biodegradation of the reference substance (Sodium benzoate) attained 64.2% after Day 5, 95.3% after Day 14 and 93.6% after Day 28.

In the Toxicity Control (TC) test mixture, 41.3% degradation occurred within 14 days and the value exceeded 25% based on total ThOD, which indicated that the test item can be assumed to be not inhibitory under the conditions of this test.
In the Abiotic Sterile Control (ASC) Test Vessel, 17.2% degradation occurred after 28 days.

Based on the results, the percentage biodegradation within 28 days did not exceed the pass level of 60% ThOD. The test item did not meet the criteria for 'ready biodegradability' under the described test conditions.