Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 500-046-6 | CAS number: 26183-52-8 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the category approach justification provided in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 140 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: Source, key, 9002-92-0, Sasol, 1997, BH-97/04, activated sludge
- Duration:
- 16.9 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Source, supporting, 68439-50-9, Sasol, 1996, PZ-96/06, P. putida
- Duration:
- 5 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: Source, supporting, 68439-50-9, Sasol, 1996, SK-93/17, P. putida
- Executive summary:
The toxicity to microorganisms is assessed on the basis of data from three studies with similar alcohol ethoxylates (C12, 4 EO, CAS 9002-92-0, C12 -14, 2 EO, CAS 68439 -50 -9 and C12-14, 6 EO, CAS 68439-50-9).
In a respiration test with domestic activated sludge according to EG-guideline 88/302/EG and GLP (Diefenbach, 1997), an EC50 (3h) of 140 mg/L was determined. This finding is supported by the results of a two tests with Pseudomonas putida that revealed an EC50 (16.9 hours) > 10 g/L and an EC50 (5 h) > 2 mg/l.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14 March 1997
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: EG Guideline 88/302/EG
- Version / remarks:
- 30.05.1988
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Trade name: Marlowet BL
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- All test vessels were supplemented with 200 µl Baysilone as defoaming agent and vigorously stirred prior to the amendment of sludge.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Approximately 5 L activated sludge were taken from the municipal sewage treatment plant Marl-West, Germany, on the day before test start. The sludge was sedimented and washed with 1.5 L tap water and aerated for 10 min after additional sendimentation. The sludge was then suspended in 4 L of tap water and 200 mL synthetic sewage and was aerated and stirred. On the day of the test, the sludge material was washed twice and resuspended in 3 L tap water and had a dry weight of 3.82 g/L.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- No details given
- Test temperature:
- 20 ± 2 °C
- pH:
- test end: 8.1 - 8.2 in test substance and controls, 8.4 - 8.5 in reference controls
- Dissolved oxygen:
- test end at start of O2 measurement: 6.14 - 8.56 mg/L
- Nominal and measured concentrations:
- Nominal: 25, 50, 100, 150, 200 mg/L
- Details on test conditions:
- The test solutions in the vessels were continously stirred and aerated with clean, oil-free air (flow rate 0.5 - 1 L/min) to maintain a minimal concentration of 2.5 mg/L dissolved oxygen. All vessels contained 16 mL synthetic sewage and 200 mL inoculum and were filled to 500 mL with tap water after amendment of the emulsified test substance.
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Respiration rate after 3 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2x - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 140 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Controls: The difference between the two controls without test substance was 1.1%
- At the time of measurement the O2 consumption curve exhibited linearity - Results with reference substance (positive control):
- - Results with reference substance valid: yes
- Relevant effect levels: EC50 = 6.7 mg/L - Conclusions:
- An EC50 of 140 mg/l was found out for the inhibition of respiration of activated sludge.
- Executive summary:
In a respiration test with domestic activated sludge according to EG-guideline 88/302/EG and GLP, an EC50 (3 h) of 140 mg/L was determined.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 13 - 14 NOV 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Pseudomonas putida
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16.9 h
- Test temperature:
- 21.1 - 21.2 °C
- Nominal and measured concentrations:
- nominal: 0.1, 1, 10, 100, 1000 and 10000 mg a.s./L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: sterile 250 mL Erlenmeyer flasks
- Type (delete if not applicable): closed with cellulose stoppers
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6 growth controls, 1 pH-control, 1 blank - Reference substance (positive control):
- no
- Key result
- Duration:
- 16.9 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 16.9 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- yes
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Test 1: 19 - 20 OCT 1993; Test 2: 21 - 22 OCT 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Oxygen consumption test according to a method developed by Hüls AG
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Trade name: Marlipal 24/20
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: in-house culture originally obtained from "Institut für Wasser-, Boden- und Lufthygiene des BGA (Berlin)"
- Preparation of inoculum for exposure: 200 mL steril medium was inoculated with 80 mL bacteria suspension (liquid culture of test organism, approximately 19 h incubation time, bacterial count approximately 10 x 10 exp. 5 bacteria/mL - extinction 436 nm: 0.29 - 0.31). Inoculum was aerated and stirred for 5 min.
- Initial biomass concentration: bacterial count approximately 10 x 10 exp. 5 bacteria/mL - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 5.33 h
- Remarks on exposure duration:
- 1st test: 5.33 h; 2nd test: 5.5 h
- Test temperature:
- pre-culture:
Test 1: 24.8 - 25.0 °C
Test 2: 24.9 - 25.0 °C
incubation period:
test 1: 24.7 - 26.6 °C
test 2: 24.7 - 26.0 °C - Nominal and measured concentrations:
- nominal: 100, 500, 1000 and 2000 µg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 100 mL BSB-bottles
- No. of organisms per vessel: 10 x 10 exp. 5 bacterial count
- No. of vessels per concentration (replicates): 4 (in two of them 1 mL HgCl3 was added for determination of the degree of autoxidation)
- No. of vessels per control (replicates): 5 - Reference substance (positive control):
- no
- Duration:
- 5 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Referenceopen allclose all
Concentration |
Inhibition of cell growth (%) |
0.1 |
-0.1 |
1 |
-0.3 |
10 |
-1.8 |
100 |
-0.7 |
1000 |
-4.6 |
10000 |
-7.4 |
No dose-response relationship of the test results, thus no EC10 available
Test |
Concentration [µg/L] |
|||
100 |
500 |
1000 |
2000 |
|
1 |
11.1% |
7.3% |
5.5% |
2.6% |
2 |
11.0% |
9.6% |
5.1% |
4.5% |
Description of key information
EC50 (3h) = 140 mg/L for activated sludge
EC50 (16.9h) > 10 g for Pseudomonas putida
Key value for chemical safety assessment
- EC50 for microorganisms:
- 140 mg/L
Additional information
Since no data for decan-1-ol, ethoxylated, 1-2.5EO (CAS 26183-52-8) are available, toxicity to microorganisms is assessed on the basis of data from three studies with similar alcohol ethoxylates (C12, 4 EO; CAS 9002-92-0, C12 -14, 2 EO; CAS 68439-50-9 and C12-14, 6 EO; CAS 68439-50-9).
In a respiration test with domestic activated sludge according to EG-guideline 88/302/EG and GLP (Sasol, 1997), an EC50 (3h) of 140 mg/L was determined. This finding is supported by the results of a two tests with Pseudomonas putida that revealed an EC50 (16.9 hours) > 10 g/L and an EC50 (5 h) > 2 mg/L (Sasol, 1996 & Sasol, 1993).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.