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EC number: 687-893-6 | CAS number: 1150560-54-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31st May 2018 to 1st June 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Qualifier:
- according to guideline
- Guideline:
- other: MEP, P.R. China, the Guidelines for the Testing of Chemicals, Effects on Biotic Systems (the Second Edition), No. 209 "Activated Sludge, Respiration Inhibition Test". (2013.8).
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1-{[2-fluoro-6-(trifluoromethyl)phenyl]methyl}-5-iodo-6-methyl-1,2,3,4-tetrahydropyrimidine-2,4-dione
- EC Number:
- 687-893-6
- Cas Number:
- 1150560-54-5
- Molecular formula:
- C13H9F4IN2O2
- IUPAC Name:
- 1-{[2-fluoro-6-(trifluoromethyl)phenyl]methyl}-5-iodo-6-methyl-1,2,3,4-tetrahydropyrimidine-2,4-dione
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Details on sampling:
- DOC for total respiration was measured for 10 minutes after 3 hours contact time
Test solutions
- Details on test solutions:
- To prepare test item mixtures, the test item was directly weighed into the test vessel and distilled water was added. Four test concentrations of 10, 100, 300 and 1000 mg/L were prepared.
Test organisms
- Details on inoculum:
- The activated sludge was derived from a treatment plant receiving predominantly domestic sewage. A fresh sample of activated sludge was collected from the aeration tank of Shenyang North Sewage Treatment Plant 1 day before the test was started and the sludge was fed daily with synthetic sewage feed.
On the day of activated sludge collection, coarse particles were removed by settling for 15mins and decanting the upper layer of fine solids. The sludge was first centrifuged for 5 mins at 2500rpm to produce a clear supernatent. The supernatent liquid was discarded and the sludge resuspended in chlorine-free tap water, and the wash water removed by re-centrifuging for 5 mins and discarding again.
The sludge pellet was resuspended in chlorine free tap water. The concentration of re-suspended sludge was determined by drying 3 replicates of 10ml of suspended at 105 oC for 1hour 50mins. From the measured results, the sludge was further diluted with chlorine-free tap water to obtain the required sludge solids concentration of 3 g/L.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20.2 - 21.6 oC
- Nominal and measured concentrations:
- The definitive test was conducted with 4 concentrations of 10, 100, 300 and 1000 mg/L. 10, 100, 300 mg/L were tested with 1 replicate, 1000 mg/L was tested with triplicate and an abiotic control at concentration of 1000 mg/L was performed.
- Details on test conditions:
- Test water: distilled water
Air for aeration: clean oil-free air, flow rate 0.5 ~ 1 L/min - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrations
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Based on the results of test item test, the EC50 mg/L of test item for total respiration resulted in a total of >1000. Validity criteria were met throughout test.
- Results with reference substance (positive control):
- 3,5-dichlorophenol was used as a reference item in order to check the sensitivity of the activated sludge used as microbial inoculum on the day of exposure. The reference item was tested at 0.5, 1.3, 3.5, 9.4 and 25 mg/L with 1 replicate. Based on the results of the reference item test, the EC50 ( 3 hours) of the reference substance for the definitive test was:
EC50 (mg/L)= 36.5mg/L with a 95% confidence limit of 2.2 ~ 5.6mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results of the test item in the definitive test, the EC50 (3 hours) of test item for respiration is as follows:
Total Inhibition of respiration EC50 (mg/L) >1000 - Executive summary:
The study was conducted to determine the inhibition effects of Methyl iodouracil on micro-organisms from activated sludge (largely bacteria) by measuring their respiration rate. The contact time for exposure was 3 hours.
The test was conducted with four concentrations of 10, 100, 300 and 1000 mg/L. 10, 100, 300 mg/L were tested with 1 replicate. 1000mg/L was tested in triplicate and an abiotic control at a concentration of 1000mg/L was performed. Inoculums blank controls were performed with 4 replicates. The rate of respiration was determined after 3 hours contact time.
3,5-DCP was used as a reference item in order to check the sensitivity of the activated sludge used as a microbial inoculum on the day of exposure. The reference item was tested at 0.5, 1.3, 3.5, 9.4 and 25 mg/L with one replicate.
The specific oxygen uptake rate of the blank controls (without the test item or reference item) was 21.3 mg/(h·g SS) oxygen. The coefficient of variation of oxygen uptake rate in control replicates was 2.0 at the end of the definitive test. The validity criteria have therefore been satisfied.
Based on the results of the reference test item, the EC50 of 3,5-DCP for total respiration is as follows:
Inhibition of Respiration EC50 (mg/L) Total 3.5 Based on the results of the test item in the definitive test, the EC50 (3 hours) of test item for respiration is as follows:
Inhibition of respiration EC50 (mg/L) Total >1000
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