2-thienylacetyl chloride

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

December 1977

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

- Principle of test: 1-hr inhalation exposure
- Short description of test conditions: rats are exposed for 1 hour to 2 mg/L air and then observed during 14 days
- Parameters analysed / observed: clinical signs, mortality, body weight, autopsie

GLP compliance:

no

Test type:

fixed concentration procedure

Test material

Test material information

Constituent 1

Reference substance name:

2-thienylacetyl chloride

EC Number:

254-290-0

EC Name:

2-thienylacetyl chloride

Cas Number:

39098-97-0

Molecular formula:

C6H5ClOS

IUPAC Name:

2-thienylacetyl chloride

Test material form:

liquid

Details on test material:

- Density: 1.29

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: no data
- Females (if applicable) nulliparous and non-pregnant: no data
- Age at study initiation: no data
- Weight at study initiation: 202.8g for males and 190.2g for females on D0 (mean value)
- Fasting period before study: no data
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum): no data
- Acclimation period: no data

ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes: around 10/hours
- Photoperiod (hrs dark / hrs light): no data

IN-LIFE DATES: no data

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
A parallelepipedic glass vessel, 100L in volume, is continuously crossed by the atmosphere overloaded with the product. These vapors are obtained by creating a vaporization of the product inside a glass column: this column is fed, on the one hand, with compressed air at a known and adjustable flow rate and, on the other hand, with the liquid substance also arriving at a determined rate by means of a syringe infuser (Perfusor Braun).
This 100L vessel is crossed by the overloaded atmosphere with a flow rate of approximately 1 m3 per hour. A ventilator located inside the vessel homogenises continuously the atmosphere.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

1 h

Concentrations:

2.05 mg/L

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: weighing D0, 2, 4, 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 2.05 mg/L air

Based on:

test mat.

Exp. duration:

1 h

Effect levels 2

Sex:

male/female

Dose descriptor:

LC0

Effect level:

>= 2.05 mg/L air

Based on:

test mat.

Exp. duration:

1 h

Mortality:

no mortality

Clinical signs:

other: during the exposure: attempted escape, hyperactivity, closing of the palpebral fissure, sneezing, trembling, loose breathing movements. after the exposure: tachycardia (D0-1), hyperactivity (D0-4), then normal behavior.

Body weight:

no effect

Gross pathology:

whitish mottling and numerous red spots in the lungs in several animals.
gastric mucous irritation in one female.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

In this study in rats the 1-hr LC0 is 2.05 mg/L (no mortality is observed).

Executive summary:

In an acute inhalation toxicity study, groups of Sprague-Dawley rats (5/sex) were exposed by inhalation route to 2-thienylacetyl chloride for 1 hour (whole body) at concentration of 2.05 mg/L.  Animals then were observed for 14 days.

LC0 Combined = 2.05 mg/L (no mortality occurred)